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Revision as of 18:42, 1 November 2017

Dsign - Minimal portfolio Bootstrap template

Biobrick
Description
Length
Receptor expression
BBa_2305000 strong constitutive promoter with cycle amplification system and GFP/RFP dual fluorescent system 4739
BBa_2305001 cycle amplification system and GFP/RFP dual fluorescent system 4673
BBa_2305002 pbad(I13453) promoter with GFP/RFP dual fluorescent system 3272
BBa_2305003 pbad(I13453) promoter with GFP 2195
BBa_2305004 strong constitutive promoter pcat and plux promoter with GFP 1007
BBa_2305005 plux promoter regulated LuxR and GFP 1748
BBa_2305006 plac promoter regulated RFP with GFP 1955
BBa_2305007 GFP reporter with luxl 1547
BBa_2305008 strong constitutive promtor with plux and LuxR 928
BBa_2305009 LacI generator with GFP reporter 2057
BBa_2305010 Plac promoter regulated RFP with lacI generator and GFP 3134
BBa_2305011 GFP/RFP dual fluorescent system with pbad(I0500) promoter with araC 4352
BBa_2305012 LacI with RBS 1171
BBa_2305013 pbad promoter and LacI generator with RBS 1309
BBa_2305014 plux promoter with LuxR and luxI generator with RBS 1531
BBa_2305015 strong constitutive promoter with plux and LuxR 1597
BBa_2305016 cycle amplification system with GFP 2417
BBa_2305017 GFP with strong constitutive promoter(RBS-GFP-T-pcat) 833
BBa_2305018 strong constitutive promoter with GFP(pcat-RBS-GFP-T) 831




Surprise

 

 

a.

Meanwhile, we recovered the freeze-dried plasmid. They were transformed into the wild type E.coli. We constructed 19 plasmids, and 18 of them were successfully recovered. The result was shown as the figure.


  

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