Difference between revisions of "Team:BGIC-Union"

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<html>
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<HTML>
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  <head>
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     <meta charset="utf-8">
 
     <meta charset="utf-8">
 
    
 
    
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     <title>Team：BGIC-Union</title>
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     <title>Safety</title>
  
 
   <head>
 
   <head>
 
    
 
    
 +
        <link href='http://fonts.googleapis.com/css?family=Lato:400,300' rel='stylesheet' type='text/css'>
 +
        <link href='http://fonts.googleapis.com/css?family=Raleway:400,300,500' rel='stylesheet' type='text/css'>
  
 
   <!-- Bootstrap core CSS -->
 
   <!-- Bootstrap core CSS -->
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   <link href="https://2017.igem.org/Template:BGIC-Union/owlcarousel-css?action=raw&amp;ctype=text/css" rel="stylesheet">
 
   <link href="https://2017.igem.org/Template:BGIC-Union/owlcarousel-css?action=raw&amp;ctype=text/css" rel="stylesheet">
 
   <link href=" https://2017.igem.org/Template:BGIC-Union/fontawesomemin2-css?action=raw&amp;ctype=text/css" rel="stylesheet">
 
   <link href=" https://2017.igem.org/Template:BGIC-Union/fontawesomemin2-css?action=raw&amp;ctype=text/css" rel="stylesheet">
 +
 
  
 
   <!-- main -->
 
   <!-- main -->
−
   <link href="https://2017.igem.org/Team:BGIC-Union/finalcss/main-css?action=raw&amp;ctype=text/css" rel="stylesheet">
+
   <style type="text/css">
−
 
+
−
 
+
 
    
 
    
−
 
+
@import url(http://fonts.googleapis.com/css?family=Montserrat:400,700);
−
  <!-- script -->
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#top_title,#sideMenu { display: none !important; }
−
  <script src="https://code.jquery.com/jquery-1.10.2.min.js"></script>
+
        #content {
−
  <script type="text/javascript">
+
            width: 100%;
−
  /*
+
            margin: 0;
−
* jQuery Easing v1.3 - http://gsgd.co.uk/sandbox/jquery/easing/
+
            padding: 0;
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*
+
           
−
* Uses the built in easing capabilities added In jQuery 1.1
+
        }
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* to offer multiple easing options
+
−
*
+
−
* TERMS OF USE - jQuery Easing
+
−
*
+
−
* Open source under the BSD License.
+
−
*
+
−
* Copyright © 2008 George McGinley Smith
+
−
* All rights reserved.
+
−
*
+
−
* Redistribution and use in source and binary forms, with or without modification,
+
−
* are permitted provided that the following conditions are met:
+
−
*
+
−
* Redistributions of source code must retain the above copyright notice, this list of
+
−
* conditions and the following disclaimer.
+
−
* Redistributions in binary form must reproduce the above copyright notice, this list
+
−
* of conditions and the following disclaimer in the documentation and/or other materials
+
−
* provided with the distribution.
+
−
*
+
−
* Neither the name of the author nor the names of contributors may be used to endorse
+
−
* or promote products derived from this software without specific prior written permission.
+
−
*
+
−
* THIS SOFTWARE IS PROVIDED BY THE COPYRIGHT HOLDERS AND CONTRIBUTORS "AS IS" AND ANY
+
−
* EXPRESS OR IMPLIED WARRANTIES, INCLUDING, BUT NOT LIMITED TO, THE IMPLIED WARRANTIES OF
+
−
* MERCHANTABILITY AND FITNESS FOR A PARTICULAR PURPOSE ARE DISCLAIMED. IN NO EVENT SHALL THE
+
−
*  COPYRIGHT OWNER OR CONTRIBUTORS BE LIABLE FOR ANY DIRECT, INDIRECT, INCIDENTAL, SPECIAL,
+
−
*  EXEMPLARY, OR CONSEQUENTIAL DAMAGES (INCLUDING, BUT NOT LIMITED TO, PROCUREMENT OF SUBSTITUTE
+
−
*  GOODS OR SERVICES; LOSS OF USE, DATA, OR PROFITS; OR BUSINESS INTERRUPTION) HOWEVER CAUSED
+
−
* AND ON ANY THEORY OF LIABILITY, WHETHER IN CONTRACT, STRICT LIABILITY, OR TORT (INCLUDING
+
−
*  NEGLIGENCE OR OTHERWISE) ARISING IN ANY WAY OUT OF THE USE OF THIS SOFTWARE, EVEN IF ADVISED
+
−
* OF THE POSSIBILITY OF SUCH DAMAGE.
+
−
*
+
−
*/
+
  
−
// t: current time, b: begInnIng value, c: change In value, d: duration
 
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jQuery.easing['jswing'] = jQuery.easing['swing'];
 
  
−
jQuery.extend( jQuery.easing,
+
 
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{
+
 
−
def: 'easeOutQuad',
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#content{
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swing: function (x, t, b, c, d) {
+
  background-color: #FFe4e1; !important;
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//alert(jQuery.easing.default);
+
}
−
return jQuery.easing[jQuery.easing.def](x, t, b, c, d);
+
 
−
},
+
 
−
easeInQuad: function (x, t, b, c, d) {
+
−
return c*(t/=d)*t + b;
+
html, body {
−
},
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    margin: 0 !important;
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easeOutQuad: function (x, t, b, c, d) {
+
    padding: 0 !important;
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return -c *(t/=d)*(t-2) + b;
+
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},
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if ((t/=d/2) < 1) return c/2*t*t + b;
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+
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+
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easeInQuart: function (x, t, b, c, d) {
+
−
return c*(t/=d)*t*t*t + b;
+
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+
−
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+
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+
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easeInOutQuart: function (x, t, b, c, d) {
+
−
if ((t/=d/2) < 1) return c/2*t*t*t*t + b;
+
−
return -c/2 * ((t-=2)*t*t*t - 2) + b;
+
−
},
+
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easeInQuint: function (x, t, b, c, d) {
+
−
return c*(t/=d)*t*t*t*t + b;
+
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+
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return c*((t=t/d-1)*t*t*t*t + 1) + b;
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−
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+
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+
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+
−
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+
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+
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+
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+
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+
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+
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+
−
if (a < Math.abs(c)) { a=c; var s=p/4; }
+
−
else var s = p/(2*Math.PI) * Math.asin (c/a);
+
−
if (t < 1) return -.5*(a*Math.pow(2,10*(t-=1)) * Math.sin( (t*d-s)*(2*Math.PI)/p )) + b;
+
−
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+
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+
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+
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+
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+
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+
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+
−
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easeInBounce: function (x, t, b, c, d) {
+
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return c - jQuery.easing.easeOutBounce (x, d-t, 0, c, d) + b;
+
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+
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return c*(7.5625*t*t) + b;
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if (t < d/2) return jQuery.easing.easeInBounce (x, t*2, 0, c, d) * .5 + b;
+
−
return jQuery.easing.easeOutBounce (x, t*2-d, 0, c, d) * .5 + c*.5 + b;
+
 
}
 
}
−
});
+
body {
 +
  background-color: #FFe4e1;
 +
  font-family: 'Montserrat', sans-serif;
 +
    font-weight: 400;
 +
    font-size: 14px;
 +
    color: #555;
 +
    ；
 +
    -webkit-font-smoothing: antialiased;
 +
    -webkit-overflow-scrolling: touch;
 +
}
 +
 
 +
/* Titles */
 +
h1, h2, h3, h4, h5, h6 {
 +
font-family: 'Montserrat', sans-serif;
 +
    font-weight: 700;
 +
    color: #333;
 +
}
 +
 
 +
h1 {
 +
font-size: 35px;
 +
margin-top: 30px;
 +
margin-bottom: 30px;
 +
}
 +
 
 +
 
 +
 
 +
/* Paragraph &amp; Typographic */
 +
p {
 +
    line-height: 28px;
 +
    margin-bottom: 25px;
 +
    font-size: 16px;
 +
}
 +
 
 +
.centered {
 +
    text-align: center;
 +
}
 +
 
 +
/* Links */
 +
a {
 +
    color: #20B2AA;
 +
    word-wrap: break-word;
 +
 
 +
    -webkit-transition: color 0.1s ease-in, background 0.1s ease-in;
 +
    -moz-transition: color 0.1s ease-in, background 0.1s ease-in;
 +
    -ms-transition: color 0.1s ease-in, background 0.1s ease-in;
 +
    -o-transition: color 0.1s ease-in, background 0.1s ease-in;
 +
    transition: color 0.1s ease-in, background 0.1s ease-in;
 +
}
 +
 
 +
a:hover,
 +
a:focus {
 +
    color: #7b7b7b;
 +
    text-decoration: none;
 +
    outline: 0;
 +
}
 +
 
 +
a:before,
 +
a:after {
 +
    -webkit-transition: color 0.1s ease-in, background 0.1s ease-in;
 +
    -moz-transition: color 0.1s ease-in, background 0.1s ease-in;
 +
    -ms-transition: color 0.1s ease-in, background 0.1s ease-in;
 +
    -o-transition: color 0.1s ease-in, background 0.1s ease-in;
 +
    transition: color 0.1s ease-in, background 0.1s ease-in;
 +
}
 +
 
 +
hr {
 +
    display: block;
 +
    height: 1px;
 +
    border: 0;
 +
    border-top: 1px solid #ccc;
 +
    margin: 1em 0;
 +
    padding: 0;
 +
}
 +
 
 +
.copyrights{
 +
text-indent:-9999px;
 +
height:0;
 +
line-height:0;
 +
font-size:0;
 +
overflow:hidden;
 +
}
 +
 
 +
.navbar {
 +
text-transform: uppercase;
 +
margin-bottom: 0px;
 +
}
 +
 
 +
.navbar-inverse {
 +
padding-bottom: 70px;
 +
padding-top: 70px;
 +
}
 +
 
 +
.navbar-inverse {
 +
background-color: #FFA07A;
 +
border-color: #FA8072;
 +
}
 +
 
 +
.navbar-inverse .navbar-nav > li > a {
 +
color: white;
 +
}
 +
 
 +
.navbar-inverse .navbar-nav > li > a:hover {
 +
color: #222222;
 +
}
 +
 
 +
.navbar-brand {
 +
font-weight: 700;
 +
font-size: 20px;
 +
letter-spacing: 2px;
 +
}
 +
 
 +
.navbar-inverse .navbar-brand {
 +
color: white;
 +
}
 +
 
 +
.navbar-inverse .navbar-toggle {
 +
border-color: transparent;
 +
}
 +
 
 +
.progress-bar-theme {
 +
  background-color: #FFA07A;
 +
}
 +
 
 +
 
 +
/* Helpers */
 +
 
 +
.mt {
 +
margin-top: 40px;
 +
margin-bottom: 40px;
 +
}
 +
 
 +
.pt {
 +
padding-top: 50px;
 +
padding-bottom: 50px;
 +
}
 +
 
 +
 
 +
/* +++++ WRAP SECTIONS +++++ */
 +
 
 +
#ww {
 +
padding-top: 0px;
 +
padding-bottom: 70px;
 +
background-color: #f2f2f2;
 +
}
 +
 
 +
 
 +
#footer {
 +
background-color: #ADD8E6;
 +
padding-top: 50px;
 +
padding-bottom: 50px;
 +
}
 +
 
 +
#footer p {
 +
color: white;
 +
}
 +
 
 +
#footer h4 {
 +
color: white;
 +
text-transform: uppercase;
 +
padding-bottom: 20px;
 +
}
 +
 
 +
 
 +
/* +++++ Color Wraps / Blog Page +++++ */
 +
 
 +
#grey {
 +
padding-top: 60px;
 +
padding-bottom: 60px;
 +
background-color: #f2f2f2;
 +
}
 +
 
 +
#white {
 +
padding-top: 60px;
 +
padding-bottom: 60px;
 +
background-color: #ffffff;
 +
}
 +
 
 +
 
 +
 
 +
/* Blog Date*/
 +
bd {
 +
font-size: 12px;
 +
text-transform: uppercase;
 +
color: #d2d2d2;
 +
font-weight: 700;
 +
}
 +
 
 +
/* Blog Author*/
 +
ba {
 +
font-size: 12px;
 +
text-transform: uppercase;
 +
}
 +
 
 +
/* Blog Quote */
 +
.bq {
 +
font-size: 22px;
 +
padding-top: 30px;
 +
}
 +
 
 +
/* Blog Tags */
 +
bt {
 +
font-size: 12px;
 +
}
 +
 
 +
/* ================== PORTFOLIO IMAGES HOVER EFFECT ================== */
 +
/* Effects also are controled by hover.zoom.js */
 +
.zoom {
 +
    display:block;
 +
    position:relative;
 +
    overflow:hidden;
 +
    background:transparent url(https://static.igem.org/mediawiki/2017/f/fe/Bgic_loader.gif) no-repeat center;
 +
}
 +
 
 +
 
 +
    .zoomOverlay {
 +
        position:absolute;
 +
        top:0; left:0;
 +
        bottom:0; right:0;
 +
        display:none;
 +
        background-image:url(../img/zoom.png);
 +
        background-repeat:no-repeat;
 +
        background-position:center;
 +
    }
 +
 
 +
.zoom2 {
 +
opacity: 1;
 +
}
 +
 
 +
.zoom2:hover {
 +
opacity: 0.5;
 +
}
 +
 
 +
/* glyphicon Configuration */
  
−
/*
+
.glyphicon {
−
*
+
font-size: 40px;
−
* TERMS OF USE - EASING EQUATIONS
+
color: #1abc9c;
−
*
+
}
−
* Open source under the BSD License.
+
  </style>
−
*
+
 
−
* Copyright © 2001 Robert Penner
+
−
* All rights reserved.
+
−
*
+
−
* Redistribution and use in source and binary forms, with or without modification,
+
−
* are permitted provided that the following conditions are met:
+
−
*
+
−
* Redistributions of source code must retain the above copyright notice, this list of
+
−
* conditions and the following disclaimer.
+
−
* Redistributions in binary form must reproduce the above copyright notice, this list
+
−
* of conditions and the following disclaimer in the documentation and/or other materials
+
−
* provided with the distribution.
+
−
*
+
−
* Neither the name of the author nor the names of contributors may be used to endorse
+
−
* or promote products derived from this software without specific prior written permission.
+
−
*
+
−
* THIS SOFTWARE IS PROVIDED BY THE COPYRIGHT HOLDERS AND CONTRIBUTORS "AS IS" AND ANY
+
−
* EXPRESS OR IMPLIED WARRANTIES, INCLUDING, BUT NOT LIMITED TO, THE IMPLIED WARRANTIES OF
+
−
* MERCHANTABILITY AND FITNESS FOR A PARTICULAR PURPOSE ARE DISCLAIMED. IN NO EVENT SHALL THE
+
−
*  COPYRIGHT OWNER OR CONTRIBUTORS BE LIABLE FOR ANY DIRECT, INDIRECT, INCIDENTAL, SPECIAL,
+
−
*  EXEMPLARY, OR CONSEQUENTIAL DAMAGES (INCLUDING, BUT NOT LIMITED TO, PROCUREMENT OF SUBSTITUTE
+
−
*  GOODS OR SERVICES; LOSS OF USE, DATA, OR PROFITS; OR BUSINESS INTERRUPTION) HOWEVER CAUSED
+
−
* AND ON ANY THEORY OF LIABILITY, WHETHER IN CONTRACT, STRICT LIABILITY, OR TORT (INCLUDING
+
−
*  NEGLIGENCE OR OTHERWISE) ARISING IN ANY WAY OUT OF THE USE OF THIS SOFTWARE, EVEN IF ADVISED
+
−
* OF THE POSSIBILITY OF SUCH DAMAGE.
+
−
*
+
−
*/
+
−
</script>
+
 
    
 
    
 
   <script src="https://2017.igem.org/Template:BGIC-Union/newbootstrap-js?action=raw&amp;ctype=text/javascript"></script>
 
   <script src="https://2017.igem.org/Template:BGIC-Union/newbootstrap-js?action=raw&amp;ctype=text/javascript"></script>
−
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Revision as of 19:11, 1 November 2017

Safety

Hi, I am dCasentry,

a T7—dCas9 multi-output DNA sensor designed to detect ctDNA! Cancer is a leading cause of death worldwide, accounting for 8.8 million deaths in 2015, and the most common cause of cancer death is lung cancer (1.69 million deaths). Nowadays, multiple lung cancer detection methods are developed, one of them being liquid biopsy. The technique of liquid biopsy is much better than the popular biopsy as it obviates pain and complication incurred to patients by detecting various tumor markup, including short strand circulating tumor DNA (CtDNA) in the blood. However, it still requires laboratory apparatus such as PCR instrument.
Thus our primary task is to devise a more efficient, rapid and convenient lung cancer prognosis method. We construct a paper chip containing powerful biosensor capable of detecting the fusion gene, EML4-ALK, which only exists only in NSCLC (non small cell lung cancer) patients’ blood. Specific targeted drug for this mutation has been produced, which means patients can receive precise treatment as soon as possible when the detection result is positive.
The biosensor, dCasentry, consists of paired dCas9 protein guided by sgRNA and linked with split T7 RNA polymerase that serves as a switch. When the targeting lung cancer mutation gene is detected, visible signal will be released as the T7 RNA polymerase starts transcription. DCasentry not only deals with low ctDNA concentration in blood but also capable of producing various kinds of signal output, such as GFP and RFP.
We adopt freeze-dried paper chip as the vector of the detection system to simplify its operation as well as storage. The DNA device and proteins needed for detection are mixed with cell-free system and freeze-dried on the paper chip. During testing, the blood sample of the patient provides a liquid environment to reactivate the cell-free system. The product will be presented as a kit, containing including hemostix, a hand power centrifuge, NASBA reaction system and the paper screening chip, that can be widely used on the medical field.

Please, click here and know more about me as well as the product!

Best Parts

Applied Design

Integrated Human Practice

Education & Public Engagement

Entreprenuership

Model