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− | {{CSMU_NCHU_Taiwan}} | + | {{:Team:CSMU_NCHU_Taiwan/Templates/Header}} |
− | <html> | + | <html lang="en"> |
+ | <head> | ||
+ | <meta charset="UTF-8"> | ||
+ | <link href='https://fonts.googleapis.com/css?family=Arizonia' rel='stylesheet'> | ||
+ | <link href='https://fonts.googleapis.com/css?family=Open+Sans' rel='stylesheet'> | ||
+ | <title>Safety</title> | ||
+ | </head> | ||
+ | <style type="text/css"> | ||
+ | * { | ||
+ | margin: 0; | ||
+ | padding: 0; | ||
+ | box-sizing: border-box; } | ||
− | + | body { | |
− | + | width: 100%; | |
− | + | margin: 0; | |
− | + | padding: 0; | |
− | + | font-family: 'Ubuntu' !important; } | |
− | + | ||
+ | img.center { | ||
+ | display: block; | ||
+ | margin: 0 auto; } | ||
+ | .pdfbtn { | ||
+ | width: 100%; | ||
+ | margin: 20px 0 10px 0; | ||
+ | padding: 10px 20px; | ||
+ | border-radius: 5px; | ||
+ | background-color: #556b7e; | ||
+ | color: white; | ||
+ | display: block; | ||
+ | position: relative; } | ||
+ | .pdfbtn i { | ||
+ | position: absolute; | ||
+ | right: 50px; | ||
+ | top: 50%; | ||
+ | transform: translateY(-50%); } | ||
+ | .brown-btn { | ||
+ | background-color: #603813; } | ||
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+ | .img-container { | ||
+ | width: 100%; | ||
+ | text-align: center; | ||
+ | display: none; } | ||
+ | .img-container img { | ||
+ | width: 100%; } | ||
− | + | .pdf-container { | |
− | + | width: 100%; | |
− | + | text-align: center; | |
+ | height: 90vh; | ||
+ | display: none; } | ||
− | + | .top { | |
+ | position: fixed; | ||
+ | right: 50px; | ||
+ | bottom: 50px; | ||
+ | height: 50px; | ||
+ | width: 50px; | ||
+ | cursor: pointer; } | ||
+ | .top img { | ||
+ | width: 100%; } | ||
− | + | #HQ_page .text-area p { | |
− | + | font-size: 22px; | |
+ | font-family: 'Ubuntu'; | ||
+ | font-weight: 500; } | ||
− | + | .bg-container { | |
+ | overflow: hidden; | ||
+ | position: relative; | ||
+ | background-color: #c5d7d9; | ||
+ | background-image: url(https://static.igem.org/mediawiki/2017/1/11/T--CSMU_NCHU_Taiwan--PartBg.png); | ||
+ | background-repeat: repeat-y; | ||
+ | background-size: 100%; | ||
+ | } | ||
− | + | .my-main-container { | |
− | + | width: 100%; | |
− | + | padding: 50px 7%; | |
− | + | } | |
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− | + | ||
− | + | .main-content { | |
+ | background-color: white; | ||
+ | width: 80%; | ||
+ | margin-left: 10%; | ||
+ | padding: 50px; | ||
+ | min-height: 180vh; | ||
+ | z-index: 10; } | ||
+ | .text-area { | ||
+ | margin-bottom: 80px; } | ||
+ | .text-area h1 { | ||
+ | font-size: 60px; | ||
+ | font-weight: 700; | ||
+ | font-family: 'Arizonia' !important; | ||
+ | text-align: center; | ||
+ | margin-bottom: 1rem; } | ||
+ | .text-area p { | ||
+ | font-size: 22px; | ||
+ | font-weight: 500; } | ||
+ | h2{ | ||
+ | font-size:30px; | ||
+ | color:#000000 !important; | ||
+ | font-weight:bold; | ||
+ | text-align:center; | ||
+ | } | ||
+ | h3{ | ||
+ | font-size:24px; | ||
+ | color:#000000 !important; | ||
+ | font-weight:bold; | ||
+ | text-align:left; | ||
+ | } | ||
+ | |||
+ | |||
+ | </style> | ||
+ | <body> | ||
+ | <div class="bg-container" style="max-height:none;"> | ||
+ | <div class="my-main-container"> | ||
+ | <div class="main-content"> | ||
+ | <div class="text-area"> | ||
+ | <h1>Composite Parts</h1> | ||
+ | <p>This year, we CSMU_NCHU_Taiwan, brings you 3 composite parts.</p> | ||
+ | |||
+ | <br> | ||
+ | <img class="center" src="https://static.igem.org/mediawiki/2017/4/4a/Csmuxnchu_parts1.png" style="width:90%;" alt=""> | ||
+ | |||
+ | <br> | ||
+ | <img class="center" src="https://static.igem.org/mediawiki/2017/0/0e/Csmuxnchu_part_form2.png" style="width:90%;" alt=""> | ||
+ | |||
+ | |||
+ | |||
+ | <h2>Composite Parts</h2> | ||
+ | <h3>FOR ANTIDOTE</h3> | ||
+ | <p><br> | ||
+ | <b>T7 promoter + Thioredoxin-FGD fusion protein (<a href="http://parts.igem.org/Part:BBa_K2382005" style="color:#385e66;"target="_blank">BBa_K2382005</a>)</b><br> | ||
+ | This part contains a T7 promoter and Thioredoxin-FGD fusion protein, and terminators are | ||
+ | included. By ligating these two different parts as a fusion protein, it is supposed to raise the | ||
+ | solubility of our protein, F420-dependent glucose-6- phosphate dehydrogenase (FGD).<br><br> | ||
+ | <b>T7 promoter + Thioredoxin-MSMEG_5998 fusion protein (<a href="http://parts.igem.org/Part:BBa_K2382006"style="color:#385e66;" target="_blank">BBa_K2382006</a>)</b><br> | ||
+ | This part contains a T7 promoter and Thioredoxin-MSMEG_5998 fusion protein, and terminators | ||
+ | are included. By ligating these two different parts as a fusion protein, it works better than | ||
+ | MSMEG_5998 alone. | ||
+ | </p> | ||
+ | <h3>FOR TEST STRIP</h3> | ||
+ | <p><br> | ||
+ | <b>Anti-aflatoxin scFv fusion protein construction DNA sequence (<a href="http://parts.igem.org/Part:BBa_K2382010"style="color:#385e66;" target="_blank">BBa_K2382010</a>)</b><br> | ||
+ | By ligating the constitutive promoter (BBa_J23101), strong ribosome binding site (BBa_B0034) | ||
+ | and scFv-EAAAK- RFP-Histag, we were able to detect aflatoxin by the purified protein expressing | ||
+ | in E. coli. Moreover, we designed a restriction site, BamHI, between scFv and EAAAK, so future | ||
+ | iGEM teams could take advantage of this composite part to fuse their scFv with RFP as indicator, | ||
+ | and make their own test strip! | ||
+ | </p> | ||
+ | </p> | ||
</div> | </div> | ||
+ | </div> | ||
+ | </div> | ||
+ | </div> | ||
+ | <div class="top"> | ||
+ | <img src="https://static.igem.org/mediawiki/2017/5/52/T--CSMU_NCHU_Taiwan--top.png" alt=""> | ||
+ | </div> | ||
+ | </body> | ||
+ | <script type="text/javascript"> | ||
+ | $('#parts-btn-1').on('click', function(){ | ||
+ | $('#parts-1').slideToggle("slow"); | ||
+ | }); | ||
+ | $('#parts-btn-2').on('click', function(){ | ||
+ | $('#parts-2').slideToggle("slow"); | ||
+ | }); | ||
+ | $('.top').on('click', function(){ | ||
+ | $('html, body').animate({scrollTop: '0px'}, 500); | ||
+ | }); | ||
+ | </script> | ||
</html> | </html> |
Latest revision as of 20:21, 1 November 2017
Composite Parts
This year, we CSMU_NCHU_Taiwan, brings you 3 composite parts.
Composite Parts
FOR ANTIDOTE
T7 promoter + Thioredoxin-FGD fusion protein (BBa_K2382005)
This part contains a T7 promoter and Thioredoxin-FGD fusion protein, and terminators are
included. By ligating these two different parts as a fusion protein, it is supposed to raise the
solubility of our protein, F420-dependent glucose-6- phosphate dehydrogenase (FGD).
T7 promoter + Thioredoxin-MSMEG_5998 fusion protein (BBa_K2382006)
This part contains a T7 promoter and Thioredoxin-MSMEG_5998 fusion protein, and terminators
are included. By ligating these two different parts as a fusion protein, it works better than
MSMEG_5998 alone.
FOR TEST STRIP
Anti-aflatoxin scFv fusion protein construction DNA sequence (BBa_K2382010)
By ligating the constitutive promoter (BBa_J23101), strong ribosome binding site (BBa_B0034)
and scFv-EAAAK- RFP-Histag, we were able to detect aflatoxin by the purified protein expressing
in E. coli. Moreover, we designed a restriction site, BamHI, between scFv and EAAAK, so future
iGEM teams could take advantage of this composite part to fuse their scFv with RFP as indicator,
and make their own test strip!