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− | We designed two constructs, known as biobricks, to allow the bacteria to express the proteins lipase and esterase that will degrade PET plastics. These constructs are identical except for the sequence for the enzyme each construct would produce: esterase and lipase. Our constructs were designed as follows. | + | We designed two constructs, known as biobricks, to allow the bacteria to express the proteins lipase and esterase that will degrade PET plastics. These constructs are identical except for the sequence for the enzyme each construct would produce: esterase and lipase. The chlorogenate esterase construct (BBa_K2436001) was cloned successfully and submitted to the registry. The lipase construct was not cloned successfully. Our constructs were designed as follows. |
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<img src="https://static.igem.org/mediawiki/2017/a/a5/T--Baltimore_Bio-Crew--small_construct_diagrams.png" style="width: 1000px; height:562px; margin-left:auto;margin-right:auto; display: block;" > | <img src="https://static.igem.org/mediawiki/2017/a/a5/T--Baltimore_Bio-Crew--small_construct_diagrams.png" style="width: 1000px; height:562px; margin-left:auto;margin-right:auto; display: block;" > |
Revision as of 20:32, 1 November 2017
![BUGSS Logo](https://static.igem.org/mediawiki/2017/e/e2/Baltimore_biocre_BugssLOGO.png)
BALTIMORE BIO-CREW
Bio-Engineering E.Coli To Degrade Plastic and Save The Baltimore Inner Harbor
Composite Parts
![](https://static.igem.org/mediawiki/2017/a/a5/T--Baltimore_Bio-Crew--small_construct_diagrams.png)