Difference between revisions of "Team:IIT Delhi/Circuit Design"

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Revision as of 21:11, 1 November 2017

iGEM IIT Delhi

Overview Squarewave Generator Interlab
Circuit design and construction Microfluidics and Fluroscence Photobleaching Promoter strength Oscillations
Basic Parts Composite Parts Improved Parts Part Collection
Overview Writing a Model Deterministic Model Stochastic Model Bifurcation and Squareness Resource sharing
Human Practices Integrated Practices Public Engagement Collaborations Safety
Overview GMM Legislation iGEM Berlin Mentoring IISER Mohali iGEM Glasgow
Lab Records Protocols Recipes Medal Criteria
The Team Attributions

CIRCUIT DESIGN AND CONSTRUCTION

                                                                                                                                                                                                                 

Two circuits were central to our work done in the project. The first one was the 5n1, which was a 5 node oscillator constructed by Richard Murray et al, that was not modified to produce square waves. The 5n1 (560) was a gift from Richard Murray (Addgene plasmid # 70730), and had the following topology and circuit –

Further, apart from this system, that was ordered for testing our characterization and measurement devices, we designed our own novel 5 node ring oscillator, which contained modifications that are mentioned at the end of the section. The topology and circuit that we designed and constructed are as follows –

This circuit was constructed in part through cloning, and in part through synthesis via IDT and Genscript. Further, the salient features of this circuit are –
  1. Cooperativity of repressors – Cooperativity was central to our work done, in ensuring that the rise and fall is fast for our circuit. The cooperativity of the repressors in the system that we have constructed is –
    2. PART NAME BIOBRICK PART TYPE
    BBa_K2525015 pSrpR : SrpR repressible promoter Promoter
    BBa_K2525016 pPhlF : PhlF repressible promoter Promoter




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Contact Us
Address

E-mail: iitd.igem@gmail.com
Undergraduate Laboratory
Department of Biotechnology and Biochemical Engineering, IIT Delhi