Difference between revisions of "Team:TUDelft/Main-Parts"

Revision as of 21:20, 1 November 2017

When constructing our parts, we made use of the IDT synthesis offer. This way we were able to generate our required parts in a fast and efficient way. All of the parts we synthesised contain the coding sequence, RBS, promoter and terminators, if applicable. Therefore, these parts are composite. The different basic parts comprising the composite parts are documented separately, and also exist as separate biobricks. This is advantageous for those wishing to combine one of our basic parts with a different expression level or compose a different composite part altogether. These basic parts were generated by using PCR and restriction ligation on the synthesised composite parts. We created a part collection comprised of 4 composite parts encoding for different Tardigrade Proteins (TDPs) and the 4 basic parts of these genes, all with elaborate characterisation so the user may choose the best protein for their project accordingly. For the composite parts of the TDPs we designed a new, strong RBS. This part does not exist separately. Additionally, we include the documentation of a basic part encoding the gene for Cas13a, derived from the plasmid pC011 from Gootenberg et al (2017). This biobrick does not exist separately. Finally, we submitted a composite part containing a nonsense spacer with flanking double repeats (with constitutive promoter), appropriate for use for Cas13a. This part is designed so that the user can easily change the spacer according to the sequence they wish to target. Find more info on this cool part on its page in the registry! We also documented this as a basic part without a promoter. This part does not exist separately. Our favourite basic part is BBa_K2306003 and our favourite composite part is BBa_K2306008.

Improved BioBrick / Further Characterisation

In our project, we added to the characterisation of an existing Biobrick, the TolR, Periplasmic domain design by Gavin Sutton from iGEM Australia 2016. Additionally, our TorA-GFP composite part was based on the biobrick created by NTU Trontheim with some alterations, such as the removal of the RFP sequence. This enabled us to build on their knowledge and improve the function.

All Parts

Fave Part	Name	Type	Description	Designer	Length
	BBa_K2306000	Coding	TorA-GFP fusion	Gabriella Tany	846
	BBa_K2306001	Composite	TorA-GFP fusion (LacI promoter)	Gabriella Tany	1212
	BBa_K2306002	Coding	Secretory-abundant heat soluble protein 68234 (SAHS 68234)	Guillermo Serena Ruiz	525
♥	BBa_K2306003	Coding	Secretory-abundant heat soluble protein 33020 (SAHS 33020)	Guillermo Serena Ruiz	509
	BBa_K2306005	Coding	Cytosolic-abundant heat soluble protein 106094 (CAHS 106094)	Guillermo Serena Ruiz	684
	BBa_K2306006	Coding	Cytosolic-abundant heat soluble protein 94205 (CAHS 94205)	Guillermo Serena Ruiz	681
	BBa_K2306007	Composite	Secretory-abundant heat soluble protein 68234 (SAHS 68234) (T7 promoter, RBS and double terminator)	Guillermo Serena Ruiz	768
♥	BBa_K2306008	Composite	Secretory-abundant heat soluble protein 33020 (SAHS 33020) (T7 promoter, RBS and double terminator)	Guillermo Serena Ruiz	752
	BBa_K2306010	Composite	Cytosolic-abundant heat soluble protein 106094 (CAHS 106094) (T7 promoter, RBS and double terminator	Guillermo Serena Ruiz	927
	BBa_K2306011	Composite	Cytosolic-abundant heat soluble protein 94205 (CAHS 94205) (T7 promoter, RBS and double terminator)	Guillermo Serena Ruiz	924
	BBa_K2306012	Coding	Cas13a	Jeroen Jacques	3920
	BBa_K2306013	Composite	Cas13a spacer with flanking double repeats, constitutive promoter	Jeroen Jacques and Jasper Veerman	134
	BBa_K2306014	RBS	RBS from bacteriophage T7 gene 10 and Shine Dalgarno	Guillermo Serena Ruiz	30
	BBa_K2306015	Other	Cas13a spacer with flanking double repeats	Jeroen Jacques and Jasper Veerman	99

Basic Parts

Fave Part	Name	Type	Description	Designer	Size (bp)
	BBa_K2306002	Basic	Secretory-abundant heat soluble protein (SAHS) 68234	Guillermo Serena Ruiz	525
♥	BBa_K2306003	Basic	Secretory-abundant heat soluble protein (SAHS) 33020	Guillermo Serena Ruiz	509
	BBa_K2306005	Basic	Cytosolic-abundant heat soluble protein (CAHS) 106094	Guillermo Serena Ruiz	684
	BBa_K2306006	Basic	Cytosolic-abundant heat soluble protein (CAHS) 94205	Guillermo Serena Ruiz	681
	BBa_K23006000	Basic	TorA GFP	Gabriella Tany	681
	BBa_K2306012	Basic	Cas13a	Jeroen Jacques	3456
	BBa_K2306014	RBS	RBS from bacteriophage T7 gene 10 and Shine Dalgarno	Guillermo Serena Ruiz	30
	BBa_K2306015	Basic	Spacer with flanking double repeats for Cas13a	Jeroen Jacques, Jasper Veerman	99

Composite Parts

Fave Part	Name	Type	Description	Designer	Size (bp)
	BBa_K2306007	Composite	Secretory-abundant heat soluble protein (SAHS) 68234 with inducible T7 promoter, RBS and double terminator	Guillermo Serena Ruiz	833
♥	BBa_K2306008	Composite	Secretory-abundant heat soluble protein (SAHS) 33020 with inducible T7 promoter, RBS and double terminator	Guillermo Serena Ruiz	817
	BBa_K2306010	Composite	Cytosolic-abundant heat soluble protein (CAHS) 106094 with inducible T7 promoter, RBS and double terminator	Guillermo Serena Ruiz	992
	BBa_K2306011	Composite	Cytosolic-abundant heat soluble protein (CAHS) 94205 with inducible T7 promoter, RBS and double terminator	Guillermo Serena Ruiz	989
	BBa_K2306001	Composite	TorA GFP fusion with inducible LacI promoter, RBS and double terminator	Gabriella Tany	681
	BBa_K2306015	Composite	Cas13a spacer with flanking double repeats, constitutive promoter	Jeroen Jacques, Jasper Veerman	134

Part Collection

Fave Part	Name	Type	Description	Designer	Size (bp)
	BBa_K2306007	Composite	Secretory-abundant heat soluble protein (SAHS) 68234 with inducible T7 promoter, RBS and double terminator	Guillermo Serena Ruiz	833
♥	BBa_K2306008	Composite	Secretory-abundant heat soluble protein (SAHS) 33020 with inducible T7 promoter, RBS and double terminator	Guillermo Serena Ruiz	817
	BBa_K2306010	Composite	Cytosolic-abundant heat soluble protein (CAHS) 106094 with inducible T7 promoter, RBS and double terminator	Guillermo Serena Ruiz	992
	BBa_K2306011	Composite	Cytosolic-abundant heat soluble protein (CAHS) 94205 with inducible T7 promoter, RBS and double terminator	Guillermo Serena Ruiz	989
	BBa_K2306002	Basic	Secretory-abundant heat soluble protein (SAHS) 68234	Guillermo Serena Ruiz	525
♥	BBa_K2306003	Basic	Secretory-abundant heat soluble protein (SAHS) 33020	Guillermo Serena Ruiz	509
	BBa_K2306005	Basic	Cytosolic-abundant heat soluble protein (CAHS) 106094	Guillermo Serena Ruiz	684
	BBa_K2306006	Basic	Cytosolic-abundant heat soluble protein (CAHS) 94205	Guillermo Serena Ruiz	681

@@ Line 12: / Line 12: @@
   <h3 id="Improved_Part-scroll">Improved BioBrick / Further Characterisation</h3>
              <p>
-In our project, we added to the characterisation of <a href=http://parts.igem.org/wiki/index.php?title=Part:BBa_K2022002 target="_blank">an existing Biobrick</a>, the TorA, Periplasmic domain design by Gavin Sutton from iGEM Australia 2016. Additionally, our TorA-GFP composite part was based on the <a href=http://parts.igem.org/Part:BBa_K1082001 target="_blank">biobrick created by NTU Trontheim</a> with some alterations, such as the removal of the RFP sequence. This enabled us to build on their knowledge and improve the function.
+In our project, we added to the characterisation of <a href=http://parts.igem.org/wiki/index.php?title=Part:BBa_K2022002 target="_blank">an existing Biobrick</a>, the TolR, Periplasmic domain design by Gavin Sutton from iGEM Australia 2016. Additionally, our TorA-GFP composite part was based on the <a href=http://parts.igem.org/Part:BBa_K1082001 target="_blank">biobrick created by NTU Trontheim</a> with some alterations, such as the removal of the RFP sequence. This enabled us to build on their knowledge and improve the function.
 </p>