Difference between revisions of "Team:BOKU-Vienna/Notebookdata"
| Line 54: | Line 54: | ||
<h1>Thursday, July 6th.</h1> | <h1>Thursday, July 6th.</h1> | ||
<ul> | <ul> | ||
| − | <li>Team discussion, | + | <li>Team discussion, planning and fundraising</li> |
<li>Counting colonies of Transformants for the Interlab Challenge: [HIER LINK ZU ERGEBNISSEN]</li> | <li>Counting colonies of Transformants for the Interlab Challenge: [HIER LINK ZU ERGEBNISSEN]</li> | ||
<ul><li>Colonies 200µL: 77, 20µL: 26, 100µL: 43</li></ul> | <ul><li>Colonies 200µL: 77, 20µL: 26, 100µL: 43</li></ul> | ||
Revision as of 13:13, 7 July 2017
Monday, July 3rd.
- Team discussion and overview:
- Christina, Felicia, Josef, Lisa, Magdalena, Mathias, Melanie, Michael, Mina, Rebecca, Valentin, Julian
- Planning of the procedures including primer design
- Preparing the growth media and autoclaving [LINK ZUM PROTOKOLL]
- LB medium preparation (4L): Mina, Michael
- Autoclaving: Mina, Rebecca
Tuesday, July 4th.
- Planning and fundraising:
- Christina, Felicia, Josef, Lisa, Martin, Mathias, Melanie, Michael, Mina, Rebecca, Valentin, Julian
- Preparation of growth media: [LINK ZUM PROTOKOLL]
- 20 plates LB medium + chloramphenicol: Mina, Lisa, Mathias
- 2 flasks LB agar: Mina, Lisa, Mathias
Wednesday, July 5th.
- Planning and fundraising:
- Christina, Felicia, Josef, Lisa, Magdalena, Martin, Mathias, Melanie, Michael, Mina, Rebecca, Julian
- Aliquoting 1mL LB into Eppendorf tubes
- Lisa, Josef
- Transferring Backbones onto agar plates:
- Julian
- Interlab challenge - transformation: According to protocol (Preparation of chemically competent E. Coli with RbCl and transformation [HIER LINK]):
- 8 transformations - from Kit Plate 6 InterLab Part Locations
- 8 plasmids plated onto agar medium: Positive control (50µL, 200µL), Negative Control (50µL, 200µL), Transformant (20µL, 100µL, 200µL), Test device 1-6 (50µL, 200µL)
- stored at 35°C
- Lisa, Mathias, Michael, Mina, Rebecca
Thursday, July 6th.
- Team discussion, planning and fundraising
- Counting colonies of Transformants for the Interlab Challenge: [HIER LINK ZU ERGEBNISSEN]
- Colonies 200µL: 77, 20µL: 26, 100µL: 43
- Miniprep of 8 plasmids for Golden Gate Standard - according to protocol [HIER LINK]
- Magdalena (1), Melanie (2), Valentin (3), Rebeccca (4), Christina (5), Martin (6), Mina (7), Lisa (8)
- Dilution of plasmids: Lisa
Friday, July 7th.
- Team discussion, Planning and Fundraising
- Interlab Challenge: Fluorescein fluorescence standard curve
- Christina
