Difference between revisions of "Team:BOKU-Vienna/Notebookdata"

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     <li>Miniprep of 8 plasmids for Golden Gate Standard - according to protocol [HIER LINK]</li>
 
     <li>Miniprep of 8 plasmids for Golden Gate Standard - according to protocol [HIER LINK]</li>
 
       <ul><li>Magdalena (1), Melanie (2), Valentin (3), Rebeccca (4), Christina (5), Martin (6), Mina (7), Lisa (8)</li></ul>
 
       <ul><li>Magdalena (1), Melanie (2), Valentin (3), Rebeccca (4), Christina (5), Martin (6), Mina (7), Lisa (8)</li></ul>
−
       <ul><li>Dilution of plasmids: Lisa</li></ul>
+
       <ul><li>Dilution of plasmids: Lisa [LINK ZU DILUTION SHEET]</li></ul>
 
   </ul>
 
   </ul>
 
</div>
 
</div>

Revision as of 13:13, 7 July 2017

Monday, July 3rd.

  • Team discussion and overview:
    • Christina, Felicia, Josef, Lisa, Magdalena, Mathias, Melanie, Michael, Mina, Rebecca, Valentin, Julian
  • Planning of the procedures including primer design
  • Preparing the growth media and autoclaving [LINK ZUM PROTOKOLL]
    • LB medium preparation (4L): Mina, Michael
    • Autoclaving: Mina, Rebecca

Tuesday, July 4th.

  • Planning and fundraising:
    • Christina, Felicia, Josef, Lisa, Martin, Mathias, Melanie, Michael, Mina, Rebecca, Valentin, Julian
  • Preparation of growth media: [LINK ZUM PROTOKOLL]
    • 20 plates LB medium + chloramphenicol: Mina, Lisa, Mathias
    • 2 flasks LB agar: Mina, Lisa, Mathias

Wednesday, July 5th.

  • Planning and fundraising:
    • Christina, Felicia, Josef, Lisa, Magdalena, Martin, Mathias, Melanie, Michael, Mina, Rebecca, Julian
  • Aliquoting 1mL LB into Eppendorf tubes
    • Lisa, Josef
  • Transferring Backbones onto agar plates:
    • Julian
  • Interlab challenge - transformation: According to protocol (Preparation of chemically competent E. Coli with RbCl and transformation [HIER LINK]):
      • Lisa, Mathias, Michael, Mina, Rebecca
    • 8 transformations - from Kit Plate 6 InterLab Part Locations
    • 8 plasmids plated onto agar medium: Positive control (50µL, 200µL), Negative Control (50µL, 200µL), Transformant (20µL, 100µL, 200µL), Test device 1-6 (50µL, 200µL)
    • stored at 35°C

Thursday, July 6th.

  • Team discussion, planning and fundraising
  • Counting colonies of Transformants for the Interlab Challenge: [HIER LINK ZU ERGEBNISSEN]
    • Colonies 200µL: 77, 20µL: 26, 100µL: 43
  • Miniprep of 8 plasmids for Golden Gate Standard - according to protocol [HIER LINK]
    • Magdalena (1), Melanie (2), Valentin (3), Rebeccca (4), Christina (5), Martin (6), Mina (7), Lisa (8)
    • Dilution of plasmids: Lisa [LINK ZU DILUTION SHEET]

Friday, July 7th.

  • Team discussion, Planning and Fundraising
  • Interlab Challenge: Fluorescein fluorescence standard curve
    • Christina