Difference between revisions of "Team:BIT/Safety"

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{{BIT}}
 
 
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<head>
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<style>
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#sideMenu,#top_title{
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display:none;
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}
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#content{
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width:100%;margin:0px;padding:0px;
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<meta http-equiv="X-UA-Compatible" content="IE=edge">
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<meta name="viewport" content="width=device-width, initial-scale=1">
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<!-- 上述3个meta标签*必须*放在最前面, 任何其他内容都*必须*跟随其后！ -->
  
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<h1> Safety </h1>
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<title>Dsign - Minimal portfolio Bootstrap template</title>
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<p>Please visit <a href="https://2017.igem.org/Safety">the main Safety page</a> to find this year's safety requirements & deadlines, and to learn about safe & responsible research in iGEM.</p>
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<link rel="stylesheet" href="https://2017.igem.org/Template:BIT/bootstrap/min/CSS?action=raw&amp;ctype=text/css"/>
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<link rel="stylesheet" href="https://2017.igem.org/Template:BIT/flexslider/CSS?action=raw&amp;ctype=text/css"/>
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<link rel="stylesheet" href="https://2017.igem.org/Template:BIT/jquery.fancybox/CSS?action=raw&amp;ctype=text/css"/>
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<link rel="stylesheet" href="https://2017.igem.org/Template:BIT/main/CSS?action=raw&amp;ctype=text/css"/>
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<link rel="stylesheet" href="https://2017.igem.org/Template:BIT/responsive/CSS?action=raw&amp;ctype=text/css"/>
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<link rel="stylesheet" href="https://2017.igem.org/Template:BIT/animate/min/CSS?action=raw&amp;ctype=text/css"/>
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<link rel="stylesheet" href="css/font-icon.css">
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<link rel="stylesheet" href="https://maxcdn.bootstrapcdn.com/font-awesome/4.4.0/css/font-awesome.min.css">
  
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<p>On this page of your wiki, you should write about how you are addressing any safety issues in your project. The wiki is a place where you can <strong>go beyond the questions on the safety forms</strong>, and write about whatever safety topics are most interesting in your project. (You do not need to copy your safety forms onto this wiki page.)</p>
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<!-- Animate.css -->
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<link rel="stylesheet" href="https://2017.igem.org/Template:BIT/animate/CSS?action=raw&amp;ctype=text/css"/>
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<!-- Icomoon Icon Fonts-->
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<link rel="stylesheet" href="https://2017.igem.org/Template:BIT/font-icon/CSS?action=raw&amp;ctype=text/css"/>
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<!-- Bootstrap  -->
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<link rel="stylesheet" href="https://2017.igem.org/Template:BIT/bootstrap/CSS?action=raw&amp;ctype=text/css"/>
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<!-- Flexslider  -->
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<link rel="stylesheet" href="https://2017.igem.org/Template:BIT/flexslider/CSS?action=raw&amp;ctype=text/css"/>
  
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</div>
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<!-- Theme style  -->
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<link rel="stylesheet" href="https://2017.igem.org/Template:BIT/style/CSS?action=raw&amp;ctype=text/css"/>
  
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</head>
  
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<div class="column full_size">
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<body>
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<h5>Safe Project Design</h5>
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<!-- header section -->
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<div class="fh5co-loader"></div>
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<div id="page">
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<nav class="fh5co-nav" role="navigation">
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<div class="top-menu">
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<div class="container">
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<div class="row">
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<div class="col-xs-2">
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<div id="fh5co-logo"><a href="../index.html">BIT<span>.</span></a></div>
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</div>
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<div class="col-xs-10 text-right menu-1">
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<ul>
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<li class="has-dropdown">
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<a style="color:#1b8fac">Project</a>
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<ul class="dropdown">
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<li><a href="https://2017.igem.org/Team:BIT/Design">Design</a></li>
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<li><a href="https://2017.igem.org/Team:BIT/Demonstrate">Demonstrate</a></li>
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<li><a href="https://2017.igem.org/Team:BIT/Model">Model</a></li>
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</ul>
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<a href="https://2017.igem.org/Team:BIT/achievement">Achievement</a>
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<ul class="dropdown">
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<li><a href="https://2017.igem.org/Team:BIT/Medal">Medal</a></li>
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<li><a href="https://2017.igem.org/Team:BIT/Prize">Prize</a></li>
 +
<li><a href="https://2017.igem.org/Team:BIT/Parts">Part</a></li>
 +
                                                                        <li><a href="https://2017.igem.org/Team:BIT/https://2017.igem.org/Team:BIT/Safety">Safety Form</a></li>
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</ul>
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</li>
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<li class="has-dropdown">
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<a href="https://2017.igem.org/Team:BIT/HP">Practice</a>
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<li><a href="https://2017.igem.org/Team:BIT/HP/Silver">Silver</a></li>
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<li><a href="https://2017.igem.org/Team:BIT/HP/Gold_Integrated">Gold</a></li>
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        <li><a href="https://2017.igem.org/Team:BIT/Engagement"> Engagement</a></li>
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                                                                <li><a href="https://2017.igem.org/Team:BIT/Entrepreneurship">Entreperneurship</a></li>
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</ul>
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</li>
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<li class="has-dropdown">
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<a href="https://2017.igem.org/Team:BIT/Notebook">Notebook</a>
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<ul class="dropdown">
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<li><a href="https://2017.igem.org/Team:BIT/Gallery">Gallery</a></li>
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<li><a href="2017.igem.org/Team:BIT/Track">Track</a></li>
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<li><a href="https://2017.igem.org/Team:BIT/nb">Journal</a></li>
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                                                                <li><a href="https://2017.igem.org/Team:BIT/Protocol">Protocol</a></li>
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</ul>
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</li>
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                                                        <li class="has-dropdown">
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<a href="https://2017.igem.org/Team:BIT/Team">Team</a>
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<ul class="dropdown">
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<li><a href="https://2017.igem.org/Team:BIT/Collaborations">Collaborations</a></li>
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<li><a href="https://2017.igem.org/Team:BIT/Team/Member">Member</a></li>
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<li><a href="https://2017.igem.org/Team:BIT/Attributions">Attributions</a></li>
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</ul>
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</li>
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<li class="btn-cta"><a href="https://2017.igem.org/Team:BIT"><span>Home</span></a></li>
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</ul>
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</div>
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</div>
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</div>
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</div>
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</nav>
  
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<p>Does your project include any safety features? Have you made certain decisions about the design to reduce risks? Write about them here! For example:</p>
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  <div id="fh5co-blog">
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<div class="container">
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<div class="row animate-box">
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<div class="col-md-8 col-md-offset-2 text-center fh5co-heading">
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<h2>Design</h2>
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<p><font size=4>This year's project, using microfluidic chip as a platform, equipped with genetic engineering ideas and molecular cloning means, ultimately achieve the purpose that using biosensors for biomarker detection.<p>Our chip consists of two chambers:<dr>
 +
<p>Chamber 1 is oval(long axis length:18mm, short axis length:6mm). In our design, the function of the chamber is to hold our magnetic beads (fixed with magnets) with the aptamer and provide a place for the sample to bind to the aptamer so that the the complementary strands with lysine can get detached . Our intention to design the chamber as an oval is that the elongated structure can reduce the bubble generated by the difference in flow rate between the cavity wall and the middle stream.<dr>
 +
<p>Chamber 2 is round( diameter:12mm) Within this chamber ,there are gel pillars(diameter: 0.5mm). The contents of the chamber are engineering bacteria frozen into dry powdery and trypsin. After the reaction in the chamber is carried out for a certain period of time, the mixed reaction of the culture medium and the reaction liquid flows from the upstream into the chamber under the negative pressure generated by the peristaltic pump<dr>
 +
<p>First of all, we select aptamers AP273, which has best affinity for AFP. We modified a biotin at the end of its 5’ end, which has affinity for streptavidin. Because of the presence of streptavidin-modified beads, aptamer has been locked firmly on beads. At the same time, a complementary chain was synthesized at its protein binding site. We modified an amino group at the 3’ end of the complementary chain which can be combined with lysine protected by BOC anhydride to link lysine to the complementary chain. Because Van der Waals forces between AFP and aptamers is stronger than hydrogen bond, due to the action of magnet lying at the bottom of the reaction system, aptamers are separated from complementary chain, one at the bottom, the other in the supernatant. At this point, with the action of trypsin, lysine can be separated from the complementary chain, and start his adventure.<dr>
  
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<ul>
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<p>In the second cavity, there is a lysine deficient E.coli which can hardly grow without the addition of lysine. Meanwhile, we transformed a plasmid with fluorescent gene into the E.coli ΔLysA. When the E.coli ΔLysA has received the lysine signal released from the complementary chain, it begins to grow with the expression of the fluorescent protein. In this way, we can transform the lysine signal to the fluorescence signal. Consider that the lysine concentration could be weak, we decided to use a strong promoter(BBa_J23100) and a cyclic amplifier system to enhance the signal, and a dual fluorescence system to improve the sensitivity.</font>
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<li>Choosing a non-pathogenic chassis</li>
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<li>Choosing parts that will not harm humans / animals / plants</li>
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<li>Substituting safer materials for dangerous materials in a proof-of-concept experiment</li>
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<li>Including an "induced lethality" or "kill-switch" device</li>
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</ul>
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</div>
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</div>
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</div>
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<div class="row">
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<div class="col-md-4">
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<div class="fh5co-blog animate-box">
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<a href="#" class="blog-bg" style="background-image: url(https://static.igem.org/mediawiki/2017/f/f5/BIT_Figure_About_main_page.jpg);"></a>
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<div class="blog-text">
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<h3><a href="#">Biosensor</a></h3>
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<p>1.Standardized linkers<br>
 +
<br>
 +
2.basing on the application of aptamers<br>
 +
<br>
 +
3.Achieving signal conversion from macromolecule to small molecule<br></p>
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<ul class="stuff">
  
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<div class="column half_size">
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<li><a href="https://2017.igem.org/Team:BIT/Design/Biosenor">Read More<i class="icon-arrow-right22"></i></a></li>
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<h5>Safe Lab Work</h5>
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</ul>
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</div>
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</div>
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</div>
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<div class="col-md-4">
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<div class="fh5co-blog animate-box">
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<a href="#" class="blog-bg" style="background-image: url(https://static.igem.org/mediawiki/2017/9/96/BIT_Figure_About_genetic_circiut..png);"></a>
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<div class="blog-text">
 +
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<h3><a href="#">Amplifier</a></h3>
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<p>1.Transform the lysine signal to the fluorescent signal<br>
 +
2.Use a strong promoter and a cyclic amplifier to enhance the signal<br>
 +
3.Use a dual fluorescence system to improve the sensitivity</p>
 +
<ul class="stuff">
 +
 +
<li><a href="https://2017.igem.org/Team:BIT/Design/Bio-amplifer">Read More<i class="icon-arrow-right22"></i></a></li>
 +
</ul>
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</div>
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</div>
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</div>
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<div class="col-md-4">
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<div class="fh5co-blog animate-box">
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<a href="#" class="blog-bg" style="background-image: url(https://static.igem.org/mediawiki/2017/2/28/Design_of_the_chip_.png);"></a>
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<div class="blog-text">
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<h3><a href="#">Microfluidic chip</a></h3>
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<p>1.Tiny volume and Low-cost<br>
 +
<br>
 +
2.Easily combined with external devices<br>
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<br>
 +
3.Easy to operate<br>
 +
<br>
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</p>
 +
<ul class="stuff">
 +
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<li><a href="https://2017.igem.org/Team:BIT/Design/HW">Read More<i class="icon-arrow-right22"></i></a></li>
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</ul>
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</div>
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</div>
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</div>
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</div>
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</div>
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</div>
  
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<p>What safety procedures do you use every day in the lab? Did you perform any unusual experiments, or face any unusual safety issues? Write about them here!</p>
 
  
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<div id="fh5co-started">
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    <div class="overlay"></div>
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    <div class="container">
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      <div class="row animate-box">
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        <div class="col-md-8 col-md-offset-2 text-center fh5co-heading">
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          <h2>Contact Us!</h2>
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          <p><center>Please keep reading if you want to contact us.</center></p>
 +
     
 +
        </div>
 +
      </div>
 +
    </div>
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  </div>
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  <footer id="fh5co-footer" role="contentinfo">
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    <div class="container">
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      <div class="row row-pb-md">
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        <div class="col-md-4 fh5co-widget">
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          <h4>BIT</h4>
 +
          <p>It's an excellent Team with thoughtful persons full of funs to the academic</p>
 +
        </div>
 +
        <div class="col-md-2 col-md-push-1 fh5co-widget">
 +
          <h4>Links</h4>
 +
          <ul class="fh5co-footer-links">
 +
            <li><a href="https://2017.igem.org/Team:BIT">Home</a></li>
 +
            <li><a href="https://2017.igem.org/Team:BIT/Team">Team</a></li>
 +
            <li><a href="https://2017.igem.org/Team:BIT/achievement">Achievement</a></li>
 +
            <li><a href="https://2017.igem.org/Team:BIT/Notebook">Notebook</a></li>
 +
          </ul>
 +
        </div>
 +
     
 +
        <div class="col-md-4 col-md-push-1 fh5co-widget">
 +
          <h4>Contact Information</h4>
 +
          <ul class="fh5co-footer-links">
 +
            <li><a href="">Beijing Institute of Technology No.5 Yard,Zhong Guan Cun South Street Haidian District, Beijing, CHINA</li>
 +
            <li><a href="">Wechat:iGEM_BIT</a></li>
 +
            <li><a href="">igem_bit@outlook.com</a></li>
 +
            <li><a href="">Facebook:iGEM_BIT</li>
 +
          </ul>
 +
        </div>
 +
      </div>
 +
      <div class="row copyright"> </div>
 +
    </div>
 +
  </footer>
 
</div>
 
</div>
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<div class="gototop js-top"> <a href="#" class="js-gotop"><i class="icon-arrow-up22"></i></a> </div>
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<div class="column half_size">
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<!-- JS FILES -->
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<h5>Safe Shipment</h5>
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<script src="https://ajax.googleapis.com/ajax/libs/jquery/1.11.3/jquery.min.js?action=raw&amp;ctype=text/jacascript"></script>  
−
 
+
<script src="https://2017.igem.org/Template:BIT/bootstrap/min/Javascript"></script>
−
<p>Did you face any safety problems in sending your DNA parts to the Registry? How did you solve those problems?</p>
+
<script src="https://2017.igem.org/Template:BIT/jquery/flexslider-min/Javascript"></script>
−
</div>
+
<script src="https://2017.igem.org/Template:BIT/jquery.easing/1/3/Javascript?action=raw&amp;ctype=text/javascript"></script>
−
 
+
<!-- Bootstrap -->
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<script src="https://2017.igem.org/Template:BIT/bootstrap/Javascript?action=raw&amp;ctype=text/javascript"></script>  
 +
<!-- Waypoints -->
 +
<script src="https://2017.igem.org/Template:BIT/jquery/waypoints/min/Javascript?action=raw&amp;ctype=text/javascript"></script>  
 +
<!-- Flexslider -->
 +
<script src="https://2017.igem.org/Template:BIT/jquery/flexslider-min/Javascript?action=raw&amp;ctype=text/javascript"></script>
 +
<!-- Main -->
 +
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 +
</body>
 
</html>
 
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Revision as of 22:01, 1 November 2017

Dsign - Minimal portfolio Bootstrap template

Design

This year's project, using microfluidic chip as a platform, equipped with genetic engineering ideas and molecular cloning means, ultimately achieve the purpose that using biosensors for biomarker detection.

Our chip consists of two chambers:

Chamber 1 is oval(long axis length:18mm, short axis length:6mm). In our design, the function of the chamber is to hold our magnetic beads (fixed with magnets) with the aptamer and provide a place for the sample to bind to the aptamer so that the the complementary strands with lysine can get detached . Our intention to design the chamber as an oval is that the elongated structure can reduce the bubble generated by the difference in flow rate between the cavity wall and the middle stream.

Chamber 2 is round( diameter:12mm) Within this chamber ,there are gel pillars(diameter: 0.5mm). The contents of the chamber are engineering bacteria frozen into dry powdery and trypsin. After the reaction in the chamber is carried out for a certain period of time, the mixed reaction of the culture medium and the reaction liquid flows from the upstream into the chamber under the negative pressure generated by the peristaltic pump

First of all, we select aptamers AP273, which has best affinity for AFP. We modified a biotin at the end of its 5’ end, which has affinity for streptavidin. Because of the presence of streptavidin-modified beads, aptamer has been locked firmly on beads. At the same time, a complementary chain was synthesized at its protein binding site. We modified an amino group at the 3’ end of the complementary chain which can be combined with lysine protected by BOC anhydride to link lysine to the complementary chain. Because Van der Waals forces between AFP and aptamers is stronger than hydrogen bond, due to the action of magnet lying at the bottom of the reaction system, aptamers are separated from complementary chain, one at the bottom, the other in the supernatant. At this point, with the action of trypsin, lysine can be separated from the complementary chain, and start his adventure.

In the second cavity, there is a lysine deficient E.coli which can hardly grow without the addition of lysine. Meanwhile, we transformed a plasmid with fluorescent gene into the E.coli ΔLysA. When the E.coli ΔLysA has received the lysine signal released from the complementary chain, it begins to grow with the expression of the fluorescent protein. In this way, we can transform the lysine signal to the fluorescence signal. Consider that the lysine concentration could be weak, we decided to use a strong promoter(BBa_J23100) and a cyclic amplifier system to enhance the signal, and a dual fluorescence system to improve the sensitivity.

Biosensor

1.Standardized linkers

2.basing on the application of aptamers

3.Achieving signal conversion from macromolecule to small molecule

Amplifier

1.Transform the lysine signal to the fluorescent signal
2.Use a strong promoter and a cyclic amplifier to enhance the signal
3.Use a dual fluorescence system to improve the sensitivity

Microfluidic chip

1.Tiny volume and Low-cost

2.Easily combined with external devices

3.Easy to operate

Contact Us!

Please keep reading if you want to contact us.