Difference between revisions of "Team:IISER-Mohali-INDIA/Hardware"

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<div class="data-img"><img style="float: left; padding: 40px;"  width="25%" height="30%" src="https://static.igem.org/mediawiki/2017/0/0b/T--IISER-Mohali-INDIA--hardware1.png" width="100%"></div>
 
<div class="data-img"><img style="float: left; padding: 40px;"  width="25%" height="30%" src="https://static.igem.org/mediawiki/2017/0/0b/T--IISER-Mohali-INDIA--hardware1.png" width="100%"></div>
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<div width="30%" style="float:center; padding:20px;"><h3>It has been already documented that bacteria culture can be grown on paper even in a
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<div width="30%" style="float:center; padding:20px;"><h3>It has been already documented that bacterial cultures can be grown on paper in a coordinated manner with the help of inkjet printer [1]. To do this, paper is completely
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cordinated manner with the help of inkjet paper [1]. To do this, paper is completely
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immersed in a toluene solution of polystyrene and then completely dried for making the
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immersed in toluene solution of polystyrene and then completely drying for making the
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hydrophobic surface [1]. Then, small patches are made by inkjet printer with toluene to
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hydrophobic surface [1]. Then, small patches are made by inkjet paper with toluene to
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form hydrophilic patches [1]. Agar is hydrolysed by sulphuric acid for appropriate viscosity
 
form hydrophilic patches [1]. Agar is hydrolysed by sulphuric acid for appropriate viscosity
 
and dispensed with inkjet printer [1]. Sodium hydroxide can be used to neutralise the agar
 
and dispensed with inkjet printer [1]. Sodium hydroxide can be used to neutralise the agar
 
and sodium sulphate can be removed by electrodialysis [1]. The agar is printed on the
 
and sodium sulphate can be removed by electrodialysis [1]. The agar is printed on the
 
hydrophilic patches and can be used to grow bacteria [1]. The paper is folded in a specific
 
hydrophilic patches and can be used to grow bacteria [1]. The paper is folded in a specific
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manner to be accommodated inside the device as shown in the image.</h3></div>
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manner to be accommodated inside the device as shown in the image:</h3></div>
  
  
 
<h3>Arrangement of cultured E. coli colonies after printing on a sheet containing agar [1]
 
<h3>Arrangement of cultured E. coli colonies after printing on a sheet containing agar [1]
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We intend to use this type of paper and fold it convoluted inside a packet of 0.2 microns filter paper thus creating a module as shown in the image.</h3>
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We intend to fold this paper in a convoluted manner and place it inside a packet of 0.2 micron filter paper, creating a module as shown in the image:</h3>
 
<div><img src="https://static.igem.org/mediawiki/2017/e/e3/T--IISER-Mohali-INDIA--paperrrlabel.png" style="float: left;  margin-left:20%;"  width="25%" height="25%"></div>
 
<div><img src="https://static.igem.org/mediawiki/2017/e/e3/T--IISER-Mohali-INDIA--paperrrlabel.png" style="float: left;  margin-left:20%;"  width="25%" height="25%"></div>
  
 
<br/><br/><br/><br/><br/><br/><br/><br/><br/><br/><br/><br/>
 
<br/><br/><br/><br/><br/><br/><br/><br/><br/><br/><br/><br/>
 
<div>
 
<div>
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<h3>The red section of the packet is the safe part which can be easily touched without disturbing cultures. One can hold it to pull the module outside to expose the detection part shown in yellow. If the detection shows a color change then the rest of the module can be taken out so that the co-cultured microbes can absorb the pollutants from the surrounding environment. Then the module is torn off and incinerated or disposed of as necessary.</h3></div>
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<h3>The red section of the packet is the safe part which can be easily touched without disturbing the cultures. One can hold it to pull the module outside and expose the detection part shown in yellow. If the detection shows a color change then the rest of the module can be taken out so that the co-cultured microbes can absorb pollutants from the surrounding environment. The used module can be torn off and incinerated.</h3></div>
 
<div class="data-img"><img align="left" src="https://static.igem.org/mediawiki/2017/5/5c/T--IISER-Mohali-INDIA--modulelabel.png"  width="25%" height="25%"></div><br/><br/><br/>
 
<div class="data-img"><img align="left" src="https://static.igem.org/mediawiki/2017/5/5c/T--IISER-Mohali-INDIA--modulelabel.png"  width="25%" height="25%"></div><br/><br/><br/>
 
<div class="data-img"><img align="left" src="https://static.igem.org/mediawiki/2017/7/76/T--IISER-Mohali-INDIA--paperlabel.png" style="margin-left:10%;" width="35%" height="35%"></div>
 
<div class="data-img"><img align="left" src="https://static.igem.org/mediawiki/2017/7/76/T--IISER-Mohali-INDIA--paperlabel.png" style="margin-left:10%;" width="35%" height="35%"></div>

Revision as of 22:22, 1 November 2017

gEco
Hardware






It has been already documented that bacterial cultures can be grown on paper in a coordinated manner with the help of inkjet printer [1]. To do this, paper is completely immersed in a toluene solution of polystyrene and then completely dried for making the hydrophobic surface [1]. Then, small patches are made by inkjet printer with toluene to form hydrophilic patches [1]. Agar is hydrolysed by sulphuric acid for appropriate viscosity and dispensed with inkjet printer [1]. Sodium hydroxide can be used to neutralise the agar and sodium sulphate can be removed by electrodialysis [1]. The agar is printed on the hydrophilic patches and can be used to grow bacteria [1]. The paper is folded in a specific manner to be accommodated inside the device as shown in the image:

Arrangement of cultured E. coli colonies after printing on a sheet containing agar [1] We intend to fold this paper in a convoluted manner and place it inside a packet of 0.2 micron filter paper, creating a module as shown in the image:













The red section of the packet is the safe part which can be easily touched without disturbing the cultures. One can hold it to pull the module outside and expose the detection part shown in yellow. If the detection shows a color change then the rest of the module can be taken out so that the co-cultured microbes can absorb pollutants from the surrounding environment. The used module can be torn off and incinerated.




Several of these modules are packed inside a wristband in a chain with perforated lines to tear off modules from the chain as shown in this image.

Each module is detachable and can be disposed off separately. If the pollution is more, then multiple modules can be used at once. After all the modules are consumed, the wrist band can be filled again with a new set of modules. The use of wrist band makes it user friendly and modules makes it usable multiple times.

References:

1. Srimongkon, T., Ishida, T., Igarashi, K., & Enomae, T. (2014). Development of a bacterial culture system using a paper platform to accommodate media and an ink-jet printing to dispense bacteria. Am. J. Biochem. Biotechnol, 10, 81-87.