<p>In future experiments, PDC 1 could be used in place of ARO10 to produce the decarboxylase enzyme necessary for the degradation of tryptophan to tryptophol.</p>
<p>In future experiments, PDC 1 could be used in place of ARO10 to produce the decarboxylase enzyme necessary for the degradation of tryptophan to tryptophol.</p>
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<h5>What should this page contain?</h5>
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<li> Clearly and objectively describe the results of your work.</li>
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<li> Future plans for the project. </li>
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<li> Considerations for replicating the experiments. </li>
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<h5>You should also describe what your results mean: </h5>
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<li> Interpretation of the results obtained during your project. Don't just show a plot/figure/graph/other, tell us what you think the data means. This is an important part of your project that the judges will look for. </li>
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<li> Show data, but remember all measurement and characterization data must be on part pages in the Registry. </li>
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<li> Consider including an analysis summary section to discuss what your results mean. Judges like to read what you think your data means, beyond all the data you have acquired during your project. </li>
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<h5> Project Achievements </h5>
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<p>You can also include a list of bullet points (and links) of the successes and failures you have had over your summer. It is a quick reference page for the judges to see what you achieved during your summer.</p>
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<li>A list of linked bullet points of the successful results during your project</li>
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<li>A list of linked bullet points of the unsuccessful results during your project. This is about being scientifically honest. If you worked on an area for a long time with no success, tell us so we know where you put your effort.</li>
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<h5>Inspiration</h5>
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<p>See how other teams presented their results.</p>
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<li><a href="https://2014.igem.org/Team:TU_Darmstadt/Results/Pathway">2014 TU Darmstadt </a></li>
<li><a href="https://2014.igem.org/Team:Paris_Bettencourt/Results">2014 Paris Bettencourt </a></li>
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Revision as of 22:32, 1 November 2017
Results
Liquid cultures of our bacteria were prepared in King’s Medium B supplemented with tryptophan. The culture was centrifuged after growth and the supernatant was filtered and used in HPLC. Figure 5 shows tryptophol run in the same media as our sample. Our HPLC graphs do not have the peak at 162 m/z shown in the standard.
From the HPLC results, the modified bacteria do not show production of tryptophol. One potential explanation is that there is post-translational modification of these gene products. In particular, ARO10 has been shown to be expressed without exhibiting 2-oxo acid decarboxylase activity, indicating the presence of post-translational modification that may be present in yeast cells but absent in the modified E. coli cells.
In future experiments, PDC 1 could be used in place of ARO10 to produce the decarboxylase enzyme necessary for the degradation of tryptophan to tryptophol.
