Difference between revisions of "Team:Aix-Marseille/SN"
TiboBongio (Talk | contribs) (Created page with "{{Aix-Marseille|title=M13 part improvement|toc=__NOTOC__}} *Transform 100µL TG1 with Eprc - SN (psb1C3 plasmid, 5µL) *To get an adequate number of colonies : *Into 2mL LB...") |
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| − | {{Aix-Marseille|title= | + | {{Aix-Marseille|title=SuperNova activity|toc=__NOTOC__}} |
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| + | We wanted to test the activity of [http://parts.igem.org/Part:BBa_K1491018|Supernova] on the colonies, so we made a protocol where we illuminated petri dish with a 16,5 Candela 595nm Diode (lumex ssl-lx5093syc/g) for 2 hours. | ||
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| + | With the following protocol : | ||
*Transform 100µL TG1 with Eprc - SN (psb1C3 plasmid, 5µL) | *Transform 100µL TG1 with Eprc - SN (psb1C3 plasmid, 5µL) | ||
Revision as of 22:52, 1 November 2017
SuperNova activity
We wanted to test the activity of [http://parts.igem.org/Part:BBa_K1491018|Supernova] on the colonies, so we made a protocol where we illuminated petri dish with a 16,5 Candela 595nm Diode (lumex ssl-lx5093syc/g) for 2 hours.
With the following protocol :
- Transform 100µL TG1 with Eprc - SN (psb1C3 plasmid, 5µL)
- To get an adequate number of colonies :
- Into 2mL LB add 2µL of 2 DO TG1
- Pipet up and down
- From the first dilution, take 8µL and dilute it into 1mL LB
- Spin at 5000rpm for 10min
- Remove 850µL supernatant
- Resuspend pellet (even if you can't see it)
- Spread remaining 150µL on petri dish
- Immediatly put on 590nm light / put control in dark
- After two hours, incubate overnight
- Count colonies
Made another control with RFP to show there's any decrease
| Exposed | Not exposed |
| 3672 | 1496 |
