Difference between revisions of "Team:Oxford/Overview Wet Lab"

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We spent a lot of time in the lab this summer! We cloned, characterised, wrote-up, protocolled, and generally learned a lot. This page provides links to every aspect of our wet lab, click on them to learn more.  
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<p> We spent a lot of time in the lab this summer! We cloned, characterised, wrote-up, protocolled, and generally learned a lot. This page provides links to every aspect of our wet lab, click on them to learn more. </p>
  
 
<h2>Results</h2>
 
<h2>Results</h2>
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<p>We generated mathematical models of our DNA-based system, our protein-based system, and the effects associated with the implementation of our diagnostic device on a human population. The aim of our models was to better understand our systems and improve them to become more feasible.</p>
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<p>We began by cloning our parts into the iGEM shipping vector, pSB1C3, and then proceeded with characterisation of the parts that made up each of our systems, one protein-based, the other DNA-based. There is always more do to, and our future experiments page provides information on future design-build-test cycles we would carry out if we had many more summers!</p>
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<p>We also created a software, which highlights the versatility of our device – by changing some of the DNA sequences (particularly the cleavage sites and output), our device can be used to detect various diseases. Our software aims to make it easier for other groups to use our diagnostic for other diseases.</p>
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<h2>What techniques did we use for modelling?</h2>
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<div class="row">
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<p> We employed numerous modelling techniques and equations to most accurately assess our systems. These include mass action and Michaelis-Menten kinetics, sensitivity analyses, parameter scans, stochastic modelling, and SIR and vector epidemiological modeling. Please follow the links below to see more detailed explanations of our models and to try out our software!</p><br>
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    <div class="col-sm-3">
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    <form action="https://2017.igem.org/Team:Oxford/DNA_Based_Model">
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  <button type="submit" class="btn btn-default btn-circle-lg" style="background-color: rgb(72,145,220)"><span id="button-lg-span">DNA-Based<br>System Model</span></button>
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    </form>
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    </div>
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    <div class="col-sm-3">
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    <form action="https://2017.igem.org/Team:Oxford/Protein_Based_Model">
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    <button type="submit" class="btn btn-default btn-circle-lg" style="background-color: rgb(158,206,235)"><span id="button-lg-span">Protein-Based<br>System Model</span></button>
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    </form>
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    </div>
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    <div class="col-sm-3">
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    <form action="https://2017.igem.org/Team:Oxford/Disease_Model">
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    <button type="submit" class="btn btn-default btn-circle-lg" style="background-color: rgb(68, 104, 125)"><span id="button-lg-span">Epidemiological<br>Model</span></button>
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    </form>
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    </div>
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    <div class="col-sm-3">
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    <form action="https://2017.igem.org/Team:Oxford/Software">
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    <button type="submit" class="btn btn-default btn-circle-lg" style="background-color: rgb(95, 155, 175)"><span id="button-lg-span">Software</span></button>
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    </form>
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    </div>
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</div>
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<h2>Protocols and Notebook </h2>
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<p> In order for other teams to learn from us learning from our numerous mistakes we have uploaded all our protocols that we used as a team, as well as the Benchling lab books we made when we were doing our initial cloning.</p><br>
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<div class="row">
 +
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    <div class="col-sm-3">
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    <form action="https://2017.igem.org/Team:Oxford/DNA_Based_Model">
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  <button type="submit" class="btn btn-default btn-circle-lg" style="background-color: rgb(72,145,220)"><span id="button-lg-span">DNA-Based<br>System Model</span></button>
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    </form>
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    </div>
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 +
    <div class="col-sm-3">
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    <form action="https://2017.igem.org/Team:Oxford/Protein_Based_Model">
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    <button type="submit" class="btn btn-default btn-circle-lg" style="background-color: rgb(158,206,235)"><span id="button-lg-span">Protein-Based<br>System Model</span></button>
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    </form>
 +
    </div>
 +
 +
    <div class="col-sm-3">
 +
    <form action="https://2017.igem.org/Team:Oxford/Disease_Model">
 +
    <button type="submit" class="btn btn-default btn-circle-lg" style="background-color: rgb(68, 104, 125)"><span id="button-lg-span">Epidemiological<br>Model</span></button>
 +
    </form>
 +
    </div>
 +
   
 +
    <div class="col-sm-3">
 +
    <form action="https://2017.igem.org/Team:Oxford/Software">
 +
    <button type="submit" class="btn btn-default btn-circle-lg" style="background-color: rgb(95, 155, 175)"><span id="button-lg-span">Software</span></button>
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    </form>
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    </div>
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</div>
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<h2> Interlab and Measurement </h2>
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        <p> We took part in the Interlab study for the 4th year in a row, and also created a measurement tool to make getting quantitative data from microscope images easier. </p></br>
  
 
<div class="row">
 
<div class="row">

Revision as of 23:12, 1 November 2017

Wet Lab Overview


We spent a lot of time in the lab this summer! We cloned, characterised, wrote-up, protocolled, and generally learned a lot. This page provides links to every aspect of our wet lab, click on them to learn more.

Results

We began by cloning our parts into the iGEM shipping vector, pSB1C3, and then proceeded with characterisation of the parts that made up each of our systems, one protein-based, the other DNA-based. There is always more do to, and our future experiments page provides information on future design-build-test cycles we would carry out if we had many more summers!

Protocols and Notebook

In order for other teams to learn from us learning from our numerous mistakes we have uploaded all our protocols that we used as a team, as well as the Benchling lab books we made when we were doing our initial cloning.


Interlab and Measurement

We took part in the Interlab study for the 4th year in a row, and also created a measurement tool to make getting quantitative data from microscope images easier.


How is our model integrated with the rest of our project?

Our modelling was closely interlinked with the rest of our project: it greatly informed and was informed by our design, wet lab work, and human practices. For more detail, please look at the specific modelling pages!