Difference between revisions of "Team:Calgary/InterLab/imposter"

Revision as of 19:33, 7 July 2017

Header

Introduction

The interlab study, as its name implies, is an international collaborative lab study where labs around the globe perform identical procedures so that synthetic biology can establish reliable and repeatable measurements. This year, the interlab study focused on GFP expression in the E. coli strain DH5ɑ using different ribosome binding sites and promoters. GFP was measured via fluorescence readings on a plate reader, and a standard measurement procedure was given out by iGEM that all participating teams followed. This universal protocol is critical, as it enables data to be compared with as many constant variables as possible to ensure that comparisons are accurate.

References

Rose, C., Parker, A., Jefferson, B., & Cartmell, E. (2015). The Characterization of Feces and Urine: A Review of the Literature to Inform Advanced Treatment Technology. Critical Reviews In Environmental Science And Technology, 45(17), 1827-1879. http://dx.doi.org/10.1080/10643389.2014.1000761

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-<p><b>BBa_E0040</b>   - This part is common across all of the transformed plasmids in the 2017 interlab study (minus the negative control), and codes for the production of GFP. GFP, or green fluorescent protein, is a protein native to the Aequeora victoria jellyfish, which glows green when exposed to UV light. GFP is used here as it provides a visual result that can be measured to compare expression of the GFP gene amongst the various plasmids.</p>
+<h> <b> Introduction </b> </h>
+<p> The interlab study, as its name implies, is an international collaborative lab study where labs around the globe perform identical procedures so that synthetic biology can establish reliable and repeatable measurements. This year, the interlab study focused on GFP expression in the E. coli strain DH5ɑ using different ribosome binding sites and promoters. GFP was measured via fluorescence readings on a plate reader, and a standard measurement procedure was given out by iGEM that all participating teams followed. This universal protocol is critical, as it enables data to be compared with as many constant variables as possible to ensure that comparisons are accurate.
+ </p>
 </html>