Difference between revisions of "Team:Calgary/InterLab/imposter"

Introduction

The interlab study, as its name implies, is an international collaborative lab study where labs around the globe perform identical procedures so that synthetic biology can establish reliable and repeatable measurements. This year, the interlab study focused on GFP expression in the E. coli strain DH5ɑ using different ribosome binding sites and promoters. GFP was measured via fluorescence readings on a plate reader, and a standard measurement procedure was given out by iGEM that all participating teams followed. This universal protocol is critical, as it enables data to be compared with as many constant variables as possible to ensure that comparisons are accurate.

This study involved two different ribosome binding sites tested with three different promoter regions, along with a positive and negative control test, for a total of 8 different trials. Each trial was compared based on its GFP fluorescence readings. The procedure that we followed for this study, which was distributed by iGEM,can be found here .

Provided below are the plasmid diagrams of each trial, as well as a brief summary of what each part does. All parts came from the iGEM registry, and can be studied more thoroughly on the iGEM site by clicking on their names below. Also provided are the graphs showing the absorbance and fluorescence readings from the experiment.

Parts & Plasmids

BBa_E0040

BBa_0010 and BBa_0012

Chloramphenicol resistance

BBa_B0032

BBa_B0034

BBa_J364100

BBa_J23151

BBa_R0040

BBa_J23101

BBa_J23106

BBa_J23117