Difference between revisions of "Team:Cadets2Vets/june-.html"

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<div class='WebsiteMenuBox '  style='position:absolute;left:12px;top:670px;z-index:20000;padding-left:5px;padding-right:5px;padding-top:5px;padding-bottom:5px;width:530px;height:33px;overflow:hidden;border-width:0px;border-style:solid;border-color:transparent;border-color:#000000;-webkit-border-radius:0px;-moz-border-radius:0px;border-radius:0px;width:547px;height:33px;'>  <style>.WebsiteMenuBox li, a { -webkit-transition-property:color, background; -webkit-transition-duration: 0.2s, 0.2s; -webkit-transition-timing-function: linear, ease-in;} .WebsiteMenuBox li {float:left;text-decoration: none;padding-bottom:8px;padding-top:8px;padding-right:32px;padding-left:32px;margin-bottom:1px;margin-right:1px;font-size:13.33px;color:rgb(255, 255, 255);font-family:arial, ArialRegular, helvetica, sans-serif;letter-spacing:0.15px;background-color:rgb(0, 0, 0);direction:ltr;} .WebsiteMenuBox li:hover {color:rgb(32, 32, 32);background-color:rgb(150, 150, 150);}.WebsiteMenuBox li a:link {color:rgb(255, 255, 255);}.WebsiteMenuBox li a:visited {color:rgb(255, 255, 255);}.WebsiteMenuBox li a:hover {color:rgb(32, 32, 32);}.WebsiteMenuBox li a:active {color:rgb(255, 255, 255);}.WebsiteMenuBox li:hover a {color:rgb(32, 32, 32) !important;}</style>  <ul id='topnav' style='float:left; padding: 0px; margin: 0px;'><a style='text-decoration: none;color:rgb(255, 255, 255);font-family:arial, ArialRegular, helvetica, sans-serif;font-weight:Normal;letter-spacing:0.15px;' title='Overview' href='overview.html'><li style=''>Overview</li></a><a style='text-decoration: none;color:rgb(32, 32, 32);font-family:arial, ArialRegular, helvetica, sans-serif;font-weight:Normal;letter-spacing:0.15px;' title='June&nbsp;' href='june-.html'><li style=' background-color:rgb(150, 150, 150); color:rgb(32, 32, 32); '>June&nbsp;</li></a><a style='text-decoration: none;color:rgb(255, 255, 255);font-family:arial, ArialRegular, helvetica, sans-serif;font-weight:Normal;letter-spacing:0.15px;' title='July' href='july.html'><li style=''>July</li></a><a style='text-decoration: none;color:rgb(255, 255, 255);font-family:arial, ArialRegular, helvetica, sans-serif;font-weight:Normal;letter-spacing:0.15px;' title='August' href='august.html'><li style=''>August</li></a>
 
<div class='WebsiteMenuBox '  style='position:absolute;left:12px;top:670px;z-index:20000;padding-left:5px;padding-right:5px;padding-top:5px;padding-bottom:5px;width:530px;height:33px;overflow:hidden;border-width:0px;border-style:solid;border-color:transparent;border-color:#000000;-webkit-border-radius:0px;-moz-border-radius:0px;border-radius:0px;width:547px;height:33px;'>  <style>.WebsiteMenuBox li, a { -webkit-transition-property:color, background; -webkit-transition-duration: 0.2s, 0.2s; -webkit-transition-timing-function: linear, ease-in;} .WebsiteMenuBox li {float:left;text-decoration: none;padding-bottom:8px;padding-top:8px;padding-right:32px;padding-left:32px;margin-bottom:1px;margin-right:1px;font-size:13.33px;color:rgb(255, 255, 255);font-family:arial, ArialRegular, helvetica, sans-serif;letter-spacing:0.15px;background-color:rgb(0, 0, 0);direction:ltr;} .WebsiteMenuBox li:hover {color:rgb(32, 32, 32);background-color:rgb(150, 150, 150);}.WebsiteMenuBox li a:link {color:rgb(255, 255, 255);}.WebsiteMenuBox li a:visited {color:rgb(255, 255, 255);}.WebsiteMenuBox li a:hover {color:rgb(32, 32, 32);}.WebsiteMenuBox li a:active {color:rgb(255, 255, 255);}.WebsiteMenuBox li:hover a {color:rgb(32, 32, 32) !important;}</style>  <ul id='topnav' style='float:left; padding: 0px; margin: 0px;'><a style='text-decoration: none;color:rgb(255, 255, 255);font-family:arial, ArialRegular, helvetica, sans-serif;font-weight:Normal;letter-spacing:0.15px;' title='Overview' href='overview.html'><li style=''>Overview</li></a><a style='text-decoration: none;color:rgb(32, 32, 32);font-family:arial, ArialRegular, helvetica, sans-serif;font-weight:Normal;letter-spacing:0.15px;' title='June&nbsp;' href='june-.html'><li style=' background-color:rgb(150, 150, 150); color:rgb(32, 32, 32); '>June&nbsp;</li></a><a style='text-decoration: none;color:rgb(255, 255, 255);font-family:arial, ArialRegular, helvetica, sans-serif;font-weight:Normal;letter-spacing:0.15px;' title='July' href='july.html'><li style=''>July</li></a><a style='text-decoration: none;color:rgb(255, 255, 255);font-family:arial, ArialRegular, helvetica, sans-serif;font-weight:Normal;letter-spacing:0.15px;' title='August' href='august.html'><li style=''>August</li></a>
 
</ul></div>
 
</ul></div>
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<div class='ParagraphBox '  style='position:absolute;left:520px;top:15px;z-index:10008;padding-left:5px;padding-right:5px;padding-top:5px;padding-bottom:5px;width:329px;height:523px;overflow:hidden;border-width:0px;border-style:solid;border-color:transparent;border-color:rgb(0, 0, 0);-webkit-border-radius:0px;-moz-border-radius:0px;border-radius:0px;width:316px;height:523px;overflow:auto;'><div class='innerText'><p style="margin: 0px; text-align: left; "><span style="color: rgb(192, 57, 43);">WEEK ONE</p><p style="margin: 0px; text-align: left; "><span style="color: rgb(192, 57, 43); ">Date:</p><p style="margin: 0px; text-align: left; "><span style="color: rgb(192, 57, 43); ">21 June, 2017</p><p style="margin: 0px; text-align: left;"><span style="color: rgb(255, 255, 255); font-size: 10px;">Experiments Performed:</span></p><p style="margin: 0px; text-align: left;"><span style="color: rgb(255, 255, 255); font-size: 10px;">Testing a circuit for biological competency within transformed E.coli.</span></p><p style="margin: 0px; text-align: left;">&nbsp;</p><p style="margin: 0px; text-align: left;"><span style="color: rgb(255, 255, 255); font-size: 10px;">Why We Performed them: </span></p><p style="margin: 0px; text-align: left;"><span style="color: rgb(255, 255, 255); font-size: 10px;">These experiments were performed to test for the presents of GFP within E. coli cells after exposure to Arsenate and arsenite. </span></p><p style="margin: 0px; text-align: left;">&nbsp;</p><p style="margin: 0px; text-align: left;">&nbsp;</p><p style="margin: 0px; text-align: left;"><span style="color: rgb(255, 255, 255); font-size: 10px;">Results:</span></p><p style="margin: 0px; text-align: left;"><span style="color: rgb(255, 255, 255); font-size: 10px;">The resulting cultures were inconclusive while consistently negative (no growth) at the 500uM
 
<div class='ParagraphBox '  style='position:absolute;left:520px;top:15px;z-index:10008;padding-left:5px;padding-right:5px;padding-top:5px;padding-bottom:5px;width:329px;height:523px;overflow:hidden;border-width:0px;border-style:solid;border-color:transparent;border-color:rgb(0, 0, 0);-webkit-border-radius:0px;-moz-border-radius:0px;border-radius:0px;width:316px;height:523px;overflow:auto;'><div class='innerText'><p style="margin: 0px; text-align: left; "><span style="color: rgb(192, 57, 43);">WEEK ONE</p><p style="margin: 0px; text-align: left; "><span style="color: rgb(192, 57, 43); ">Date:</p><p style="margin: 0px; text-align: left; "><span style="color: rgb(192, 57, 43); ">21 June, 2017</p><p style="margin: 0px; text-align: left;"><span style="color: rgb(255, 255, 255); font-size: 10px;">Experiments Performed:</span></p><p style="margin: 0px; text-align: left;"><span style="color: rgb(255, 255, 255); font-size: 10px;">Testing a circuit for biological competency within transformed E.coli.</span></p><p style="margin: 0px; text-align: left;">&nbsp;</p><p style="margin: 0px; text-align: left;"><span style="color: rgb(255, 255, 255); font-size: 10px;">Why We Performed them: </span></p><p style="margin: 0px; text-align: left;"><span style="color: rgb(255, 255, 255); font-size: 10px;">These experiments were performed to test for the presents of GFP within E. coli cells after exposure to Arsenate and arsenite. </span></p><p style="margin: 0px; text-align: left;">&nbsp;</p><p style="margin: 0px; text-align: left;">&nbsp;</p><p style="margin: 0px; text-align: left;"><span style="color: rgb(255, 255, 255); font-size: 10px;">Results:</span></p><p style="margin: 0px; text-align: left;"><span style="color: rgb(255, 255, 255); font-size: 10px;">The resulting cultures were inconclusive while consistently negative (no growth) at the 500uM
  
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that will need to be repeated. In parallel to the replication of the culture study, a miniprep and paper
 
that will need to be repeated. In parallel to the replication of the culture study, a miniprep and paper
  
based assay will be looked at as well utilizing the Promega S30 kit.</span></p><p style="margin: 0px; text-align: left;">&nbsp;</p><p style="margin: 0px; text-align: left;">&nbsp;</p><p style="margin: 0px; text-align: left;">&nbsp;</p><p style="margin: 0px; text-align: left; "><span style="color: rgb(192, 57, 43);">WEEK TWO</p><p style="margin: 0px; text-align: left; "><span style="color: rgb(192, 57, 43); ">Date:</p><p style="margin: 0px; text-align: left; "><span style="color: rgb(192, 57, 43); ">27 June, 2017</p><p style="margin: 0px; text-align: left;"><span style="color: rgb(255, 255, 255); font-size: 10px;">Experiments Performed:</span></p><p style="margin: 0px; text-align: left;"><span style="color: rgb(255, 255, 255); font-size: 10px;">Testing a circuit for biological competency within transformed E.coli.</span></p><p style="margin: 0px; text-align: left;">&nbsp;</p><p style="margin: 0px; text-align: left;"><span style="color: rgb(255, 255, 255); font-size: 10px;">Why We Performed them: </span></p><p style="margin: 0px; text-align: left;">&nbsp;</p><p style="margin: 0px; text-align: left;">&nbsp;</p><p style="margin: 0px; text-align: left;"><span style="color: rgb(255, 255, 255); font-size: 10px;">Results:</span></p><p style="margin: 0px; text-align: left;"><span style="color: rgb(255, 255, 255); font-size: 10px;">----</span></p><p style="margin: 0px; text-align: left;">&nbsp;</p><p style="margin: 0px; text-align: left;">&nbsp;</p><p style="margin: 0px; text-align: left;">&nbsp;</p><p style="margin: 0px; text-align: left;"><span style="color: rgb(255, 255, 255); font-size: 10px;">Moving Forward:</span></p><p style="margin: 0px; text-align: left;"><span style="color: rgb(255, 255, 255); font-size: 10px;">------</span></p><p style="margin: 0px; text-align: left;">&nbsp;</p><p style="margin: 0px; text-align: left;">&nbsp;</p><p style="margin: 0px; text-align: left;">&nbsp;</p></div>
+
based assay will be looked at as well utilizing the Promega S30 kit.</span></p><p style="margin: 0px; text-align: left;">&nbsp;</p><p style="margin: 0px; text-align: left;">&nbsp;</p><p style="margin: 0px; text-align: left;">&nbsp;</p>
 +
</div>
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 +
<div class='ParagraphBox '  style='position:absolute;left:520px;top:15px;z-index:10008;padding-left:5px;padding-right:5px;padding-top:5px;padding-bottom:5px;width:329px;height:523px;overflow:hidden;border-width:0px;border-style:solid;border-color:transparent;border-color:rgb(0, 0, 0);-webkit-border-radius:0px;-moz-border-radius:0px;border-radius:0px;width:316px;height:523px;overflow:auto;'>
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<p style="margin: 0px; text-align: left; "><span style="color: rgb(192, 57, 43);">WEEK TWO</p><p style="margin: 0px; text-align: left; "><span style="color: rgb(192, 57, 43); ">Date:</p><p style="margin: 0px; text-align: left; "><span style="color: rgb(192, 57, 43); ">27 June, 2017</p><p style="margin: 0px; text-align: left;"><span style="color: rgb(255, 255, 255); font-size: 10px;">Experiments Performed:</span></p><p style="margin: 0px; text-align: left;"><span style="color: rgb(255, 255, 255); font-size: 10px;">Testing a circuit for biological competency within transformed E.coli.</span></p><p style="margin: 0px; text-align: left;">&nbsp;</p><p style="margin: 0px; text-align: left;"><span style="color: rgb(255, 255, 255); font-size: 10px;">Why We Performed them: </span></p><p style="margin: 0px; text-align: left;">&nbsp;</p><p style="margin: 0px; text-align: left;">&nbsp;</p><p style="margin: 0px; text-align: left;"><span style="color: rgb(255, 255, 255); font-size: 10px;">Results:</span></p><p style="margin: 0px; text-align: left;"><span style="color: rgb(255, 255, 255); font-size: 10px;">----</span></p><p style="margin: 0px; text-align: left;">&nbsp;</p><p style="margin: 0px; text-align: left;">&nbsp;</p><p style="margin: 0px; text-align: left;">&nbsp;</p><p style="margin: 0px; text-align: left;"><span style="color: rgb(255, 255, 255); font-size: 10px;">Moving Forward:</span></p><p style="margin: 0px; text-align: left;"><span style="color: rgb(255, 255, 255); font-size: 10px;">------</span></p><p style="margin: 0px; text-align: left;">&nbsp;</p><p style="margin: 0px; text-align: left;">&nbsp;</p><p style="margin: 0px; text-align: left;">&nbsp;</p></div>
 
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</div>
 
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Revision as of 22:50, 7 July 2017

Cadets2Vets

June

WEEK ONE

Date:

21 June, 2017

Experiments Performed:

Testing a circuit for biological competency within transformed E.coli.

 

Why We Performed them:

These experiments were performed to test for the presents of GFP within E. coli cells after exposure to Arsenate and arsenite.

 

 

Results:

The resulting cultures were inconclusive while consistently negative (no growth) at the 500uM concentration across all three types. The remaining cultures after the initial concentration were 50/50 in terms of E. coli growth. When checked for fluorescence all samples below 100uM showed possible signal from GFP. However further analysis showed an odd mitigating variable, being that the culture media has an auto-fluorescence within the same spectra as GFP. To prove GFP signal each culture was pelleted out via centrifugation, washed with PBS twice and suspended to eliminate any chance of background signal. The results showed little to no GFP signal. However due to culture variability, this will be replicated. Glycerol stocks have also been created of the stock circuit.

 

 

 

Moving Forward:

Due to the results above due to many mitigating factors the cell may not be biologically competent. However, because of the variability between replicates of the varying culture concentrations that will need to be repeated. In parallel to the replication of the culture study, a miniprep and paper based assay will be looked at as well utilizing the Promega S30 kit.

 

 

 

WEEK TWO

Date:

27 June, 2017

Experiments Performed:

Testing a circuit for biological competency within transformed E.coli.

 

Why We Performed them:

 

 

Results:

----

 

 

 

Moving Forward:

------

 

 

 

Record of our weekly progress

Week Three

Date:

 

Experiments:

 

Why We Performed Them:

 

Results:

 

Conclusions

 

 

Week Four

 Date:

 

Experiments:

 

Why We Performed Them:

 

Results:

 

Conclusions:

 

JUNE

   NOTEBOOK

Notebook

Document the dates you worked on your project. This should be a detailed account of the work done each day for your project.

What should this page have?
  • Chronological notes of what your team is doing.
  • Brief descriptions of daily important events.
  • Pictures of your progress.
  • Mention who participated in what task.
Inspiration

You can see what others teams have done to organize their notes: