Difference between revisions of "Team:UFlorida/Results"

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<h1>Results</h1>
 
<h1>Results</h1>
  
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<p>We transformed plasmids containing ARO10 in pETDUET-1 and ADH in pCOLADUET-1 in BL21 cells. We were unable to confirm the presence of ARO9 in a DUET vector either through gel electrophoresis or through Sanger Sequencing. ARO10 in pETDUET-1 and ADH in pCOLADUET-1 were validated using Sanger Sequencing by Genewiz®  
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<p>Most of our time on the project was spent trying to get all three genes into one cell. We succeeded in transforming cells with three plasmids, theoretically containing ARO9, ARO 10 and ADH1 but were unable to confirm the presence of ARO9.</p>
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<p>We transformed plasmids containing ARO10 in pETDUET-1 and ADH1 in pCOLADUET-1 in BL21 cells. We were unable to confirm the presence of ARO9 in a DUET vector either through gel electrophoresis or through Sanger Sequencing. ARO9 encodes aromatic animo acid transferase II, which is the first enzyme in the tryptophol pathway. ARO10 in pETDUET-1 and ADH in pCOLADUET-1 were validated using Sanger Sequencing by Genewiz®  
 
</p>
 
</p>
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<p>We ran an HPLC Standard of tryptophol in King's Medium B. Tryptophol shows an elution peak at 162 m/z.  </p>
 
<p>We ran an HPLC Standard of tryptophol in King's Medium B. Tryptophol shows an elution peak at 162 m/z.  </p>
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<p>We are planning to conduct HPLC using a filtered bacterial culture, and compare the graph generated to the graph of our tryptophol standard. We are planning on having HPLC results for or poster and presentation.</p>
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<p>We are planning to conduct HPLC using a filtered bacterial culture, and compare the graph generated to the graph of our tryptophol standard. We are planning on having HPLC results for our poster and presentation.</p>
 
   
 
   
  

Revision as of 23:49, 1 November 2017

Results

Most of our time on the project was spent trying to get all three genes into one cell. We succeeded in transforming cells with three plasmids, theoretically containing ARO9, ARO 10 and ADH1 but were unable to confirm the presence of ARO9.

We transformed plasmids containing ARO10 in pETDUET-1 and ADH1 in pCOLADUET-1 in BL21 cells. We were unable to confirm the presence of ARO9 in a DUET vector either through gel electrophoresis or through Sanger Sequencing. ARO9 encodes aromatic animo acid transferase II, which is the first enzyme in the tryptophol pathway. ARO10 in pETDUET-1 and ADH in pCOLADUET-1 were validated using Sanger Sequencing by Genewiz®

We ran an HPLC Standard of tryptophol in King's Medium B. Tryptophol shows an elution peak at 162 m/z.

We are planning to conduct HPLC using a filtered bacterial culture, and compare the graph generated to the graph of our tryptophol standard. We are planning on having HPLC results for our poster and presentation.