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As a next step, this part can be implemented in the directed evolution approach of phage-assisted continuous evolution ({{#tag:html|<a href="https://2017.igem.org/Team:Heidelberg/Pace">PACE</a>}}) or in the phage-related discontinuous evolution ({{#tag:html|<a href="https://2017.igem.org/Team:Heidelberg/Predcel">PREDCEL</a>}}) approach to improve organosilicon synthesis by cytochrome engineering. | As a next step, this part can be implemented in the directed evolution approach of phage-assisted continuous evolution ({{#tag:html|<a href="https://2017.igem.org/Team:Heidelberg/Pace">PACE</a>}}) or in the phage-related discontinuous evolution ({{#tag:html|<a href="https://2017.igem.org/Team:Heidelberg/Predcel">PREDCEL</a>}}) approach to improve organosilicon synthesis by cytochrome engineering. | ||
− | {{Heidelberg/templateus/Imagesection|https://static.igem.org/mediawiki/2017/f/fd/T--Heidelberg--Compounds.png|Figure 1:| | + | {{Heidelberg/templateus/Imagesection|https://static.igem.org/mediawiki/2017/f/fd/T--Heidelberg--Compounds.png|Figure 1:|Compounds used in the project of organosilicons and its respective products|}} |
{{Heidelberg/templateus/Imagesection|https://static.igem.org/mediawiki/parts/9/92/T--Heidelberg--GCAnilineCompound.png|Figure 2:|Gas chromatogram for the reaction of educt (1) and (5) to the product (3). 11.7 minutes retention time, indicates product formation. Unconverted educts converge 6.9 and 7.2, 7.4 minutes|}} | {{Heidelberg/templateus/Imagesection|https://static.igem.org/mediawiki/parts/9/92/T--Heidelberg--GCAnilineCompound.png|Figure 2:|Gas chromatogram for the reaction of educt (1) and (5) to the product (3). 11.7 minutes retention time, indicates product formation. Unconverted educts converge 6.9 and 7.2, 7.4 minutes|}} |
Revision as of 00:13, 2 November 2017
BBa_K2398000
We present as best basic part the codon-optimized version of the cytochrome c protein derived from Rhodotermus marinus that is able to convert silicon educts to organosilicon products.![Circularization Construct](https://static.igem.org/mediawiki/2017/1/1d/T--Heidelberg--CytochromeCRMA.png)
![](https://static.igem.org/mediawiki/2017/f/fd/T--Heidelberg--Compounds.png)
Figure 1:
Compounds used in the project of organosilicons and its respective products
![](https://static.igem.org/mediawiki/parts/9/92/T--Heidelberg--GCAnilineCompound.png)
Figure 2:
Gas chromatogram for the reaction of educt (1) and (5) to the product (3). 11.7 minutes retention time, indicates product formation. Unconverted educts converge 6.9 and 7.2, 7.4 minutes
![](https://static.igem.org/mediawiki/parts/1/16/T--Heidelberg--MSAnilineCompound.png)
Figure 3:
Mass chromatogram shows the breakdown of the product (3) ethyl 2-((4-aminophenyl)dimethylsilyl)propanoate. The product itself corresponds to a mass of 251 dalton
![](https://static.igem.org/mediawiki/parts/e/ef/T--Heidelberg--GCNonAninlineCompound.png)
Figure 4:
Gas chromatogram for the reaction of educt (2) and (5) to the product (4). 9.2 minutes retention time indicates product formation.
![](https://static.igem.org/mediawiki/parts/2/2b/T--Heidelberg--MSNonAnilineCompound.png)
Figure 5:
Mass chromatogram shows the breakdown of the product (4) ethyl 2-(dimethyl(phenyl)silyl)propanoate. The product itself corresponds to a mass of 236 dalton