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| | {{Team:Baltimore Bio-Crew/CSS}} | | {{Team:Baltimore Bio-Crew/CSS}} |
| − | <!--
| + | {{Baltimore_Bio-Crew}} |
| − | Dimension by HTML5 UP
| + | |
| − | html5up.net | @ajlkn
| + | |
| − | Free for personal and commercial use under the CCA 3.0 license (html5up.net/license)
| + | |
| − | -->
| + | |
| | <html> | | <html> |
| − | <head>
| + | <style> |
| − | <title>Dimension by HTML5 UP</title>
| + | #textBox { |
| − | <meta charset="utf-8" />
| + | /* |
| − | <meta name="viewport" content="width=device-width, initial-scale=1, user-scalable=no" />
| + | margin-left: auto; |
| − | <link rel="stylesheet" href="assets/css/main.css" />
| + | margin-right: auto; |
| − | <!--[if lte IE 9]><link rel="stylesheet" href="assets/css/ie9.css" /><![endif]-->
| + | */ |
| − | <noscript><link rel="stylesheet" href="assets/css/noscript.css" /></noscript>
| + | margin: 20px; |
| − | </head>
| + | background-color: white; |
| − | <body>
| + | width: 50%; |
| | + | height: 700px; |
| | + | } |
| | + | #textBox p { |
| | + | font-size: 15px; |
| | + | font-family: "Trebuchet MS"; |
| | + | } |
| | | | |
| − | <!-- Wrapper -->
| + | </style> |
| − | <div id="wrapper">
| + | |
| | | | |
| − | <!-- Header -->
| + | <div id = "textBox"> |
| − | <header id="header">
| + | <h2>Project Description</h2> |
| − | <div class="logo">
| + | <p> |
| − | <span class="icon fa-diamond"></span>
| + | Our goal for this project is to genetically engineer E. coli bacteria that can break down plastic. These bacteria could have many different applications, such as: degrading plastic waste from labs that cannot be recycled, being used in a filter to catch and degrade micro plastic fibers from laundry, and breaking down plastic in a marine environment into harmless molecules. We made a lot of progress last year, and this year we plan to build on that progress. |
| − | </div>
| + | </p> |
| − | <div class="content">
| + | <p> |
| − | <div class="inner">
| + | While searching for solutions to the issue of plastic pollution in the Baltimore Inner Harbor, we found a paper by Yoshida et. al. describing a bacteria called Ideonella sakaiensis that was capable of degrading PET plastic into monomers. The bacteria used the enzyme PETase (chlorogenate esterase) to break down PET into MHET, and the enzyme MHETase (Lipase) to break down MHET into ethylene glycol and therephthalic acid. We decided to use the genes from this bacteria for our project. |
| − | <h1>Dimension</h1>
| + | </p> |
| − | <p >A fully responsive site template designed by <a href="https://html5up.net">HTML5 UP</a> and released<br /> | + | <p> |
| − | for free under the <a href="https://html5up.net/license">Creative Commons</a> license.</p>
| + | To avoid the safety risks of working with a relatively undocumented bacteria, we decided to take the plastic degradation genes from I. sakaiensis and put them into K12 E. coli bacteria. We chose E. coli because they are safe to work with and commonly used in the lab. Using the genetic sequence found in the paper, we designed the two plastic degrading enzymes so that they could be expressed in E. coli bacteria. We then had them synthesized and worked on putting these genes into E. coli. |
| − | </div>
| + | </p> |
| − | </div>
| + | <p> |
| − | <nav>
| + | By the end of last year’s competition, we had managed to insert the lipase gene into E.coli, but not the chlorogenate esterase gene. We confirmed that we had correctly inserted the lipase gene using colony PCR and gene sequencing, but we did not have the time to conduct additional assays, such as protein gels, to determine if the enzyme was being secreted from the bacteria. |
| − | <ul>
| + | This year, we plan to redesign the chlorogenate esterase and lipase genes so that they contain the proper tags that will allow them to be detected, and a secretion sequence. After we insert both genes into E. coli cells, we will test them to make sure they can secrete the plastic degrading enzymes and degrade PET plastic. |
| − | <li><a href="#intro">Intro</a></li>
| + | </p> |
| − | <li><a href="#work">Work</a></li>
| + | </div> |
| − | <li><a href="#about">About</a></li>
| + | |
| − | <li><a href="#contact">Contact</a></li>
| + | |
| − | <!--<li><a href="#elements">Elements</a></li>-->
| + | |
| − | </ul>
| + | |
| − | </nav>
| + | |
| − | </header>
| + | |
| | | | |
| − | <!-- Main -->
| |
| − | <div id="main">
| |
| | | | |
| − | <!-- Intro -->
| |
| − | <article id="intro">
| |
| − | <h2 class="major">Intro</h2>
| |
| − | <span class="image main"><img src="images/pic01.jpg" alt="" /></span>
| |
| − | <p>Aenean ornare velit lacus, ac varius enim ullamcorper eu. Proin aliquam facilisis ante interdum congue. Integer mollis, nisl amet convallis, porttitor magna ullamcorper, amet egestas mauris. Ut magna finibus nisi nec lacinia. Nam maximus erat id euismod egestas. By the way, check out my <a href="#work">awesome work</a>.</p>
| |
| − | <p>Lorem ipsum dolor sit amet, consectetur adipiscing elit. Duis dapibus rutrum facilisis. Class aptent taciti sociosqu ad litora torquent per conubia nostra, per inceptos himenaeos. Etiam tristique libero eu nibh porttitor fermentum. Nullam venenatis erat id vehicula viverra. Nunc ultrices eros ut ultricies condimentum. Mauris risus lacus, blandit sit amet venenatis non, bibendum vitae dolor. Nunc lorem mauris, fringilla in aliquam at, euismod in lectus. Pellentesque habitant morbi tristique senectus et netus et malesuada fames ac turpis egestas. In non lorem sit amet elit placerat maximus. Pellentesque aliquam maximus risus, vel sed vehicula.</p>
| |
| − | </article>
| |
| | | | |
| − | <!-- Work -->
| + | <!--START OF OLD CODE--> |
| − | <article id="work">
| + | <!-- |
| − | <h2 class="major">Work</h2>
| + | <div class="column full_size" > |
| − | <span class="image main"><img src=" images/ pic02. jpg" alt="" / ></ span> | + | <img src="http://placehold.it/2000x300/d3d3d3/f2f2f2"> |
| − | <p>Adipiscing magna sed dolor elit. Praesent eleifend dignissim arcu, at eleifend sapien imperdiet ac. Aliquam erat volutpat. Praesent urna nisi, fringila lorem et vehicula lacinia quam. Integer sollicitudin mauris nec lorem luctus ultrices.</p>
| + | </div> |
| − | <p>Nullam et orci eu lorem consequat tincidunt vivamus et sagittis libero. Mauris aliquet magna magna sed nunc rhoncus pharetra. Pellentesque condimentum sem. In efficitur ligula tate urna. Maecenas laoreet massa vel lacinia pellentesque lorem ipsum dolor. Nullam et orci eu lorem consequat tincidunt. Vivamus et sagittis libero. Mauris aliquet magna magna sed nunc rhoncus amet feugiat tempus.</p>
| + | |
| − | </article>
| + | |
| | | | |
| − | <!-- About -->
| |
| − | <article id="about">
| |
| − | <h2 class="major">About</h2>
| |
| − | <span class="image main"><img src="images/pic03.jpg" alt="" /></span>
| |
| − | <p>Lorem ipsum dolor sit amet, consectetur et adipiscing elit. Praesent eleifend dignissim arcu, at eleifend sapien imperdiet ac. Aliquam erat volutpat. Praesent urna nisi, fringila lorem et vehicula lacinia quam. Integer sollicitudin mauris nec lorem luctus ultrices. Aliquam libero et malesuada fames ac ante ipsum primis in faucibus. Cras viverra ligula sit amet ex mollis mattis lorem ipsum dolor sit amet.</p>
| |
| − | </article>
| |
| | | | |
| − | <!-- Contact -->
| |
| − | <article id="contact">
| |
| − | <h2 class="major">Contact</h2>
| |
| − | <form method="post" action="#">
| |
| − | <div class="field half first">
| |
| − | <label for="name">Name</label>
| |
| − | <input type="text" name="name" id="name" />
| |
| − | </div>
| |
| − | <div class="field half">
| |
| − | <label for="email">Email</label>
| |
| − | <input type="text" name="email" id="email" />
| |
| − | </div>
| |
| − | <div class="field">
| |
| − | <label for="message">Message</label>
| |
| − | <textarea name="message" id="message" rows="4"></textarea>
| |
| − | </div>
| |
| − | <ul class="actions">
| |
| − | <li><input type="submit" value="Send Message" class="special" /></li>
| |
| − | <li><input type="reset" value="Reset" /></li>
| |
| − | </ul>
| |
| − | </form>
| |
| − | <ul class="icons">
| |
| − | <li><a href="#" class="icon fa-twitter"><span class="label">Twitter</span></a></li>
| |
| − | <li><a href="#" class="icon fa-facebook"><span class="label">Facebook</span></a></li>
| |
| − | <li><a href="#" class="icon fa-instagram"><span class="label">Instagram</span></a></li>
| |
| − | <li><a href="#" class="icon fa-github"><span class="label">GitHub</span></a></li>
| |
| − | </ul>
| |
| − | </article>
| |
| | | | |
| − | <!-- Elements -->
| + | <div class="column full_size" > |
| − | <article id="elements">
| + | <h1> Welcome to iGEM 2017! </h1> |
| − | <h2 class="major">Elements</h2>
| + | <p>Your team has been approved and you are ready to start the iGEM season! </p> |
| | + | </div> |
| | | | |
| − | <section>
| + | <div class="clear"></div> |
| − | <h3 class="major">Text</h3>
| + | |
| − | <p>This is <b>bold</b> and this is <strong>strong</strong>. This is <i>italic</i> and this is <em>emphasized</em>.
| + | |
| − | This is <sup>superscript</sup> text and this is <sub>subscript</sub> text.
| + | |
| − | This is <u>underlined</u> and this is code: <code>for (;;) { ... }</code>. Finally, <a href="#">this is a link</a>.</p>
| + | |
| − | <hr />
| + | |
| − | <h2>Heading Level 2</h2>
| + | |
| − | <h3>Heading Level 3</h3>
| + | |
| − | <h4>Heading Level 4</h4>
| + | |
| − | <h5>Heading Level 5</h5>
| + | |
| − | <h6>Heading Level 6</h6>
| + | |
| − | <hr />
| + | |
| − | <h4>Blockquote</h4>
| + | |
| − | <blockquote>Fringilla nisl. Donec accumsan interdum nisi, quis tincidunt felis sagittis eget tempus euismod. Vestibulum ante ipsum primis in faucibus vestibulum. Blandit adipiscing eu felis iaculis volutpat ac adipiscing accumsan faucibus. Vestibulum ante ipsum primis in faucibus lorem ipsum dolor sit amet nullam adipiscing eu felis.</blockquote>
| + | |
| − | <h4>Preformatted</h4>
| + | |
| − | <pre><code>i = 0;
| + | |
| | | | |
| − | while (!deck.isInOrder()) {
| + | <div class="column half_size" > |
| − | print 'Iteration ' + i;
| + | <h5>Before you start: </h5> |
| − | deck.shuffle();
| + | <p> Please read the following pages:</p> |
| − | i++;
| + | <ul> |
| − | }
| + | <li> <a href="https://2017.igem.org/Competition">Competition Hub</a> </li> |
| | + | <li> <a href="https://2017.igem.org/Competition/Deliverables/Wiki">Wiki Requirements page</a></li> |
| | + | <li> <a href="https://2017.igem.org/Resources/Template_Documentation">Template documentation</a></li> |
| | + | </ul> |
| | + | </div> |
| | | | |
| − | print 'It took ' + i + ' iterations to sort the deck.';</code></pre>
| + | <div class="column half_size" > |
| − | </section>
| + | <div class="highlight"> |
| | + | <h5> Styling your wiki </h5> |
| | + | <p>You may style this page as you like or you can simply leave the style as it is. You can easily keep the styling and edit the content of these default wiki pages with your project information and completely fulfill the requirement to document your project.</p> |
| | + | <p>While you may not win Best Wiki with this styling, your team is still eligible for all other awards. This default wiki meets the requirements, it improves navigability and ease of use for visitors, and you should not feel it is necessary to style beyond what has been provided.</p> |
| | + | </div> |
| | + | </div> |
| | | | |
| − | <section>
| + | <div class="column full_size" > |
| − | <h3 class="major">Lists</h3>
| + | <h5> Wiki template information </h5> |
| | + | <p>We have created these wiki template pages to help you get started and to help you think about how your team will be evaluated. You can find a list of all the pages tied to awards here at the <a href="https://2017.igem.org/Judging/Pages_for_Awards">Pages for awards</a> link. You must edit these pages to be evaluated for medals and awards, but ultimately the design, layout, style and all other elements of your team wiki is up to you!</p> |
| | | | |
| − | <h4>Unordered</h4>
| + | </div> |
| − | <ul>
| + | |
| − | <li>Dolor pulvinar etiam.</li>
| + | |
| − | <li>Sagittis adipiscing.</li>
| + | |
| − | <li>Felis enim feugiat.</li>
| + | |
| − | </ul>
| + | |
| | | | |
| − | <h4>Alternate</h4>
| |
| − | <ul class="alt">
| |
| − | <li>Dolor pulvinar etiam.</li>
| |
| − | <li>Sagittis adipiscing.</li>
| |
| − | <li>Felis enim feugiat.</li>
| |
| − | </ul>
| |
| | | | |
| − | <h4>Ordered</h4>
| |
| − | <ol>
| |
| − | <li>Dolor pulvinar etiam.</li>
| |
| − | <li>Etiam vel felis viverra.</li>
| |
| − | <li>Felis enim feugiat.</li>
| |
| − | <li>Dolor pulvinar etiam.</li>
| |
| − | <li>Etiam vel felis lorem.</li>
| |
| − | <li>Felis enim et feugiat.</li>
| |
| − | </ol>
| |
| − | <h4>Icons</h4>
| |
| − | <ul class="icons">
| |
| − | <li><a href="#" class="icon fa-twitter"><span class="label">Twitter</span></a></li>
| |
| − | <li><a href="#" class="icon fa-facebook"><span class="label">Facebook</span></a></li>
| |
| − | <li><a href="#" class="icon fa-instagram"><span class="label">Instagram</span></a></li>
| |
| − | <li><a href="#" class="icon fa-github"><span class="label">Github</span></a></li>
| |
| − | </ul>
| |
| | | | |
| − | <h4>Actions</h4>
| |
| − | <ul class="actions">
| |
| − | <li><a href="#" class="button special">Default</a></li>
| |
| − | <li><a href="#" class="button">Default</a></li>
| |
| − | </ul>
| |
| − | <ul class="actions vertical">
| |
| − | <li><a href="#" class="button special">Default</a></li>
| |
| − | <li><a href="#" class="button">Default</a></li>
| |
| − | </ul>
| |
| − | </section>
| |
| | | | |
| − | <section>
| + | <div class="column half_size" > |
| − | <h3 class="major">Table</h3>
| + | <h5> Editing your wiki </h5> |
| − | <h4>Default</h4>
| + | <p>On this page you can document your project, introduce your team members, document your progress and share your iGEM experience with the rest of the world! </p> |
| − | <div class=" table-wrapper"> | + | <p> <a href="https://2017.igem.org/wiki/index.php?title=Team:Example&action=edit"> </a>Use WikiTools - Edit in the black menu bar to edit this page</p> |
| − | <table>
| + | |
| − | <thead>
| + | |
| − | <tr>
| + | |
| − | <th>Name</th>
| + | |
| − | <th>Description</th>
| + | |
| − | <th>Price</th>
| + | |
| − | </tr>
| + | |
| − | </thead>
| + | |
| − | <tbody>
| + | |
| − | <tr>
| + | |
| − | <td>Item One</td>
| + | |
| − | <td>Ante turpis integer aliquet porttitor.</td>
| + | |
| − | <td>29.99</td>
| + | |
| − | </tr>
| + | |
| − | <tr>
| + | |
| − | <td>Item Two</td>
| + | |
| − | <td>Vis ac commodo adipiscing arcu aliquet.</td>
| + | |
| − | <td>19.99</td>
| + | |
| − | </tr>
| + | |
| − | <tr>
| + | |
| − | <td>Item Three</td>
| + | |
| − | <td> Morbi faucibus arcu accumsan lorem.</td>
| + | |
| − | <td>29.99</td>
| + | |
| − | </tr>
| + | |
| − | <tr>
| + | |
| − | <td>Item Four</td>
| + | |
| − | <td>Vitae integer tempus condimentum.</td>
| + | |
| − | <td>19.99</td>
| + | |
| − | </tr>
| + | |
| − | <tr>
| + | |
| − | <td>Item Five</td>
| + | |
| − | <td>Ante turpis integer aliquet porttitor.</td>
| + | |
| − | <td>29.99</td>
| + | |
| − | </tr>
| + | |
| − | </tbody>
| + | |
| − | <tfoot>
| + | |
| − | <tr>
| + | |
| − | <td colspan="2"></td>
| + | |
| − | <td>100.00</td>
| + | |
| − | </tr>
| + | |
| − | </tfoot>
| + | |
| − | </table>
| + | |
| − | </div>
| + | |
| | | | |
| − | <h4>Alternate</h4>
| + | </div> |
| − | <div class="table-wrapper">
| + | |
| − | <table class="alt">
| + | |
| − | <thead>
| + | |
| − | <tr>
| + | |
| − | <th>Name</th>
| + | |
| − | <th>Description</th>
| + | |
| − | <th>Price</th>
| + | |
| − | </tr>
| + | |
| − | </thead>
| + | |
| − | <tbody>
| + | |
| − | <tr>
| + | |
| − | <td>Item One</td>
| + | |
| − | <td>Ante turpis integer aliquet porttitor.</td>
| + | |
| − | <td>29.99</td>
| + | |
| − | </tr>
| + | |
| − | <tr>
| + | |
| − | <td>Item Two</td>
| + | |
| − | <td>Vis ac commodo adipiscing arcu aliquet.</td>
| + | |
| − | <td>19.99</td>
| + | |
| − | </tr>
| + | |
| − | <tr>
| + | |
| − | <td>Item Three</td>
| + | |
| − | <td> Morbi faucibus arcu accumsan lorem.</td>
| + | |
| − | <td>29.99</td>
| + | |
| − | </tr>
| + | |
| − | <tr>
| + | |
| − | <td>Item Four</td>
| + | |
| − | <td>Vitae integer tempus condimentum.</td>
| + | |
| − | <td>19.99</td>
| + | |
| − | </tr>
| + | |
| − | <tr>
| + | |
| − | <td>Item Five</td>
| + | |
| − | <td>Ante turpis integer aliquet porttitor.</td>
| + | |
| − | <td>29.99</td>
| + | |
| − | </tr>
| + | |
| − | </tbody>
| + | |
| − | <tfoot>
| + | |
| − | <tr>
| + | |
| − | <td colspan="2"></td>
| + | |
| − | <td>100.00</td>
| + | |
| − | </tr>
| + | |
| − | </tfoot>
| + | |
| − | </table>
| + | |
| − | </div>
| + | |
| − | </section>
| + | |
| | | | |
| − | <section>
| |
| − | <h3 class="major">Buttons</h3>
| |
| − | <ul class="actions">
| |
| − | <li><a href="#" class="button special">Special</a></li>
| |
| − | <li><a href="#" class="button">Default</a></li>
| |
| − | </ul>
| |
| − | <ul class="actions">
| |
| − | <li><a href="#" class="button">Default</a></li>
| |
| − | <li><a href="#" class="button small">Small</a></li>
| |
| − | </ul>
| |
| − | <ul class="actions">
| |
| − | <li><a href="#" class="button special icon fa-download">Icon</a></li>
| |
| − | <li><a href="#" class="button icon fa-download">Icon</a></li>
| |
| − | </ul>
| |
| − | <ul class="actions">
| |
| − | <li><span class="button special disabled">Disabled</span></li>
| |
| − | <li><span class="button disabled">Disabled</span></li>
| |
| − | </ul>
| |
| − | </section>
| |
| | | | |
| − | <section>
| + | <div class="column half_size" > |
| − | <h3 class="major">Form</h3>
| + | <h5>Tips</h5> |
| − | <form method="post" action="#">
| + | <p>This wiki will be your team’s first interaction with the rest of the world, so here are a few tips to help you get started: </p> |
| − | <div class=" field half first"> | + | <ul> |
| − | <label for="demo-name">Name</label>
| + | <li>State your accomplishments! Tell people what you have achieved from the start. </li> |
| − | <input type="text" name="demo-name" id="demo-name" value="" placeholder="Jane Doe" />
| + | <li>Be clear about what you are doing and how you plan to do this.</li> |
| − | </div>
| + | <li>You have a global audience! Consider the different backgrounds that your users come from.</li> |
| − | <div class="field half">
| + | <li>Make sure information is easy to find; nothing should be more than 3 clicks away. </li> |
| − | <label for="demo-email">Email</label>
| + | <li>Avoid using very small fonts and low contrast colors; information should be easy to read. </li> |
| − | <input type="email" name="demo-email" id="demo-email" value="" placeholder="jane@untitled.tld" />
| + | < li> Start documenting your project as early as possible; don’t leave anything to the last minute before the Wiki Freeze. For a complete list of deadlines visit the <a href=" https:// 2017.igem.org/ Calendar"> iGEM 2017 calendar</ a> </li> |
| − | </div>
| + | <li>Have lots of fun! </li> |
| − | <div class="field">
| + | </ul> |
| − | <label for="demo-category">Category</label>
| + | </div> |
| − | <div class="select-wrapper">
| + | |
| − | <select name="demo-category" id="demo-category">
| + | |
| − | <option value="">-</option>
| + | |
| − | <option value="1">Manufacturing</option>
| + | |
| − | <option value="1">Shipping</option>
| + | |
| − | <option value="1">Administration</option>
| + | |
| − | <option value="1">Human Resources</option>
| + | |
| − | </select>
| + | |
| − | </div>
| + | |
| − | </div>
| + | |
| − | <div class="field half first">
| + | |
| − | <input type="radio" id="demo-priority-low" name="demo-priority" checked>
| + | |
| − | <label for="demo-priority-low">Low</label>
| + | |
| − | </div>
| + | |
| − | <div class="field half">
| + | |
| − | <input type="radio" id="demo-priority-high" name="demo-priority">
| + | |
| − | <label for="demo-priority-high">High</label>
| + | |
| − | </div>
| + | |
| − | <div class="field half first">
| + | |
| − | <input type="checkbox" id="demo-copy" name="demo-copy">
| + | |
| − | <label for="demo-copy">Email me a copy</label>
| + | |
| − | </div>
| + | |
| − | <div class="field half">
| + | |
| − | <input type="checkbox" id="demo-human" name="demo-human" checked>
| + | |
| − | <label for="demo-human">Not a robot</label>
| + | |
| − | </div>
| + | |
| − | <div class="field">
| + | |
| − | <label for="demo-message">Message</label>
| + | |
| − | <textarea name="demo-message" id="demo-message" placeholder="Enter your message" rows="6"></textarea>
| + | |
| − | </div>
| + | |
| − | <ul class="actions">
| + | |
| − | <li><input type="submit" value="Send Message" class="special" /></li>
| + | |
| − | <li><input type="reset" value="Reset" /></li>
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| − | </ul>
| + | |
| − | </form>
| + | |
| − | </section>
| + | |
| | | | |
| − | </article>
| |
| | | | |
| − | </div>
| + | <div class="column half_size" > |
| | + | <h5>Inspiration</h5> |
| | + | <p> You can also view other team wikis for inspiration! Here are some examples:</p> |
| | + | <ul> |
| | + | <li> <a href="https://2014.igem.org/Team:SDU-Denmark/"> 2014 SDU Denmark </a> </li> |
| | + | <li> <a href="https://2014.igem.org/Team:Aalto-Helsinki">2014 Aalto-Helsinki</a> </li> |
| | + | <li> <a href="https://2014.igem.org/Team:LMU-Munich">2014 LMU-Munich</a> </li> |
| | + | <li> <a href="https://2014.igem.org/Team:Michigan"> 2014 Michigan</a></li> |
| | + | <li> <a href="https://2014.igem.org/Team:ITESM-Guadalajara">2014 ITESM-Guadalajara </a></li> |
| | + | <li> <a href="https://2014.igem.org/Team:SCU-China"> 2014 SCU-China </a></li> |
| | + | </ul> |
| | + | </div> |
| | | | |
| − | <!-- Footer -->
| + | <div class="column half_size" > |
| − | <footer id="footer">
| + | <h5> Uploading pictures and files </h5> |
| − | <p class="copyright"> © Untitled. Design: < a href="https:// html5up.net"> HTML5 UP< /a>.</ p> | + | <p> You can upload your pictures and files to the iGEM 2017 server. Remember to keep all your pictures and files within your team's namespace or at least include your team's name in the file name. <br /> |
| − | </footer>
| + | When you upload, set the "Destination Filename" to <br><code>T--YourOfficialTeamName--NameOfFile.jpg</code>. (If you don't do this, someone else might upload a different file with the same "Destination Filename", and your file would be erased!)<br><br> |
| | | | |
| − | </div>
| + | <a href="https://2017.igem.org/Special:Upload"> |
| − | | + | UPLOAD FILES |
| − | <!-- BG -->
| + | </a> |
| − | <div id="bg"></div>
| + | </p> |
| − | | + | </div> |
| − | <!-- Scripts -->
| + | --> |
| − | <script src="assets/js/jquery.min.js"></script>
| + | |
| − | <script src="assets/js/skel.min.js"></script>
| + | |
| − | <script src="assets/js/util.js"></script>
| + | |
| − | <script src="assets/js/main.js"></script>
| + | |
| − | | + | |
| − | </body>
| + | |
| − | </html>
| + | |
Project Description
Our goal for this project is to genetically engineer E. coli bacteria that can break down plastic. These bacteria could have many different applications, such as: degrading plastic waste from labs that cannot be recycled, being used in a filter to catch and degrade micro plastic fibers from laundry, and breaking down plastic in a marine environment into harmless molecules. We made a lot of progress last year, and this year we plan to build on that progress.
While searching for solutions to the issue of plastic pollution in the Baltimore Inner Harbor, we found a paper by Yoshida et. al. describing a bacteria called Ideonella sakaiensis that was capable of degrading PET plastic into monomers. The bacteria used the enzyme PETase (chlorogenate esterase) to break down PET into MHET, and the enzyme MHETase (Lipase) to break down MHET into ethylene glycol and therephthalic acid. We decided to use the genes from this bacteria for our project.
To avoid the safety risks of working with a relatively undocumented bacteria, we decided to take the plastic degradation genes from I. sakaiensis and put them into K12 E. coli bacteria. We chose E. coli because they are safe to work with and commonly used in the lab. Using the genetic sequence found in the paper, we designed the two plastic degrading enzymes so that they could be expressed in E. coli bacteria. We then had them synthesized and worked on putting these genes into E. coli.
By the end of last year’s competition, we had managed to insert the lipase gene into E.coli, but not the chlorogenate esterase gene. We confirmed that we had correctly inserted the lipase gene using colony PCR and gene sequencing, but we did not have the time to conduct additional assays, such as protein gels, to determine if the enzyme was being secreted from the bacteria.
This year, we plan to redesign the chlorogenate esterase and lipase genes so that they contain the proper tags that will allow them to be detected, and a secretion sequence. After we insert both genes into E. coli cells, we will test them to make sure they can secrete the plastic degrading enzymes and degrade PET plastic.
