Difference between revisions of "Team:CSU Fort Collins/InterLab"

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<p>The Interlab Study protocols for transformation and plate reader use that were followed came directly from the iGEM website. </p>  
 
<p>The Interlab Study protocols for transformation and plate reader use that were followed came directly from the iGEM website. </p>  
 
<h2>Results</h2>
 
<h2>Results</h2>
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<p>All of the test devices, including the negative and positive control plates with growing colonies
+
<p>All of the test devices, including the negative and positive control plates with growing colonies were given to us by the University of Colorado Boulder iGEM team. We were able to successfully grow up all of the cultures and take samples at the following time points: 0, 2, 4, and 6. After collecting all of these samples, we used a plate reader to measure the optical density of our cultures. Our plate reader was not able to read the fluorescence in the format the iGEM required. As a result of having equipment malfunction, we were not allowed to submit our data collectively with other iGEM teams that participated in the Interlab study. However, we followed every procedure that iGEM had for how to do the study and we did have results, they just were no accepted by iGEM.
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were given to us by the University of Colorado Boulder team. We were unable to successfully
+
 
−
transform competent E. coli cells with all of the plasmids. After following the protocols to grow
+
−
the cultures, samples were taken from each test device at hour 0, 2, 4, and 6. Unfortunately,
+
−
the fluorescence of the colonies were not measured correctly so we were not able to
+
−
contribute to the interlab study data.
+
  
 
</p>
 
</p>

Revision as of 00:15, 2 November 2017

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Experimental Approach

The Interlab Study protocols for transformation and plate reader use that were followed came directly from the iGEM website.

Results

All of the test devices, including the negative and positive control plates with growing colonies were given to us by the University of Colorado Boulder iGEM team. We were able to successfully grow up all of the cultures and take samples at the following time points: 0, 2, 4, and 6. After collecting all of these samples, we used a plate reader to measure the optical density of our cultures. Our plate reader was not able to read the fluorescence in the format the iGEM required. As a result of having equipment malfunction, we were not allowed to submit our data collectively with other iGEM teams that participated in the Interlab study. However, we followed every procedure that iGEM had for how to do the study and we did have results, they just were no accepted by iGEM.