Difference between revisions of "Team:CMUQ/Biofilm Formation Lab R"

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<p style="font-size: 20px;margin-right:40px; margin-left:40px; ">Minimal formation of Biofilm of SRB8 and DH5 at lower NaCl concentration range (0.05-1.6 M) in artificial sea water and 2X LB </p>
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<table style="width:50%">
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<tr>
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<th></th>
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<th colspan="2" >SRB8</th>
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<th colspan="2" >DH5</th>
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</tr>
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    <th>NaCl Concentration (M)</th>
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    <th>Average Absorbance in Sea water</th>
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    <th>Average Absorbance in 2X LB</th>
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    <th>Average Absorbance in Sea water </th>
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    <th>Average Absorbance in 2X LB </th>
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  <tr>
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    <td>0</td>
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    <td>0.767666667</td>
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    <td>1.462666667</td>
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    <td>0.950333333 </td>
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    <td>3.703333333</td>
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  </tr>
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  <tr>
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    <td>0.5</td>
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    <td>1.739</td>
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    <td>1.418</td>
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    <td>1.035333333</td>
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    <td>4.210666667</td>
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  </tr>
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  <tr>
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    <td>0.1</td>
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    <td>1.1</td>
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    <td>0.936</td>
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    <td>1.585666667</td>
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    <td>4.278333333</td>
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  </tr>
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    <td>0.2</td>
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    <td>1.153666667 </td>
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    <td>0.850333333</td>
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    <td>1.793666667</td>
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    <td>4.133666667</td>
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  </tr>
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  <tr>
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    <td>0.4</td>
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    <td>1.075333333 </td>
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    <td>0.717666667</td>
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    <td>1.187333333</td>
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    <td>2.028666667</td>
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  </tr>
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  <tr>
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    <td>0.8</td>
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    <td>1.042333333 </td>
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    <td>1.658666667</td>
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    <td>1.142666667</td>
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    <td>2.156666667</td>
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  </tr>
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  <tr>
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  <td>1.6</td>
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  <td>0.843666667</td>
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  <td>0.658666667</td>
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  <td>1.926333333</td>
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  <td>1.350333333</td>
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  </tr>
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</table>
 
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Revision as of 00:21, 2 November 2017

Biofilm Lab Results

Biofilm formation was tested under conditions similar to that of seawater conditions using artificial seawater prepared in lab, which contains main salts and concentrations found in seawater. This test was done as a control to observe biofilm formation under standard conditions of artificial seawater.

Results indicated that biofilm was formed since absorbance is directly related to biofilm formation, and any increase in the absorbance is directly related to biofilm formation. We have found also that DH5 is capable of forming biofilm.

Different samples Average Absorbance Standard Deviation
DH5 0.2615 0.07797649
SRB5A 1.1185 0.16140529
SRB7 1.3985 0.13931619
SRB8 1.44375 0.25075137

Minimal formation of Biofilm of SRB8 and DH5 at lower NaCl concentration range (0.05-1.6 M) in artificial sea water and 2X LB

SRB8 DH5
NaCl Concentration (M) Average Absorbance in Sea water Average Absorbance in 2X LB Average Absorbance in Sea water Average Absorbance in 2X LB
0 0.767666667 1.462666667 0.950333333 3.703333333
0.5 1.739 1.418 1.035333333 4.210666667
0.1 1.1 0.936 1.585666667 4.278333333
0.2 1.153666667 0.850333333 1.793666667 4.133666667
0.4 1.075333333 0.717666667 1.187333333 2.028666667
0.8 1.042333333 1.658666667 1.142666667 2.156666667
1.6 0.843666667 0.658666667 1.926333333 1.350333333