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+ | <p><a href=”https://static.igem.org/mediawiki/2017/6/69/T-NUDT_CHINA-EXPERIMENT1.pdf”>Protocol for Annealing</a></p> | ||
+ | <p><a href=”https://static.igem.org/mediawiki/2017/e/e5/T-NUDT_CHINA-EXPERIMENT3.pdf”>Protocol for Ligation</a></p> | ||
+ | <p><a href=”https://static.igem.org/mediawiki/2017/5/55/T-NUDT_CHINA-EXPERIMENT5.pdf”>Protocol for CCK8 (Cell Counting kit-8)</a></p> | ||
+ | <p><a href=”https://static.igem.org/mediawiki/2017/2/2e/T-NUDT_CHINA-EXPERIMENT6.pdf”>Protocol for Lipofectamine® 2000 Transfection</a></p> | ||
− | < | + | <p><a href=”https://static.igem.org/mediawiki/2017/3/3d/T-NUDT_CHINA-EXPERIMENT7.pdf”>Protocol for Transwell cell migration</a></p> |
− | <a href=”https://static.igem.org/mediawiki/2017/ | + | |
− | < | + | |
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− | <a href=”https://static.igem.org/mediawiki/2017/ | + | <p><a href=”https://static.igem.org/mediawiki/2017/c/c9/T-NUDT_CHINA-EXPERIMENT8.pdf”>Protocol for Western blotting</a></p> |
− | <a href=”https://static.igem.org/mediawiki/2017/ | + | <p><a href=”https://static.igem.org/mediawiki/2017/7/7e/T-NUDT_CHINA-EXPERIMENT9.pdf”>Protocol for cell culture and subculture</a></p> |
− | <a href=”https://static.igem.org/mediawiki/2017/ | + | <p><a href=”https://static.igem.org/mediawiki/2017/2/24/T-NUDT_CHINA-EXPERIMENT2.pdf”>Protocol for Asymmetrical PCR</a></p> |
− | <a href=”https://static.igem.org/mediawiki/2017/ | + | <p><a href=”https://static.igem.org/mediawiki/2017/9/91/T-NUDT_CHINA-EXPERIMENT19.PDF”>Protocol for PCR with PrimeStar HS DNA polymerase</a></p> |
− | <a href=”https://static.igem.org/mediawiki/2017/ | + | <p><a href=”https://static.igem.org/mediawiki/2017/d/df/T-NUDT_CHINA-EXPERIMENT13.pdf”>Protocol for Phosphorylation Modification</a></p> |
− | <a href=”https://static.igem.org/mediawiki/2017/ | + | <p><a href=”https://static.igem.org/mediawiki/2017/d/d3/T-NUDT_CHINA-EXPERIMENT20.PDF”>Protocol for colony PCR</a></p> |
− | <a href=”https://static.igem.org/mediawiki/2017/d/ | + | <p><a href=”https://static.igem.org/mediawiki/2017/d/d0/T-NUDT_CHINA-EXPERIMENT21.PDF”>Protocol for Agarose Gel Electrophoresis</a></p> |
− | <a href=”https://static.igem.org/mediawiki/2017/ | + | <p><a href=”https://static.igem.org/mediawiki/2017/0/09/T-NUDT_CHINA-EXPERIMENT22.PDF”>Protocol for double digestion</a></p> |
− | <a href=”https://static.igem.org/mediawiki/2017/ | + | <p><a href=”https://static.igem.org/mediawiki/2017/4/40/T-NUDT_CHINA-EXPERIMENT23.PDF”>Protocol for Gel Purification with OMEGA Gel Extraction Kit</a></p> |
− | <a href=”https://static.igem.org/mediawiki/2017/ | + | <p><a href=”https://static.igem.org/mediawiki/2017/3/3e/T-NUDT_CHINA-EXPERIMENT25.PDF”>Protocol for mini-prep with OMEGA E.Z.N.A.® Plasmid DNA Mini Kit I.</a></p> |
− | < | + | <p><a href=”https://static.igem.org/mediawiki/2017/2/2f/T-NUDT_CHINA-EXPERIMENT26.PDF”>Protocol for transformation with ligation reaction system with TIANGEN DH5a competent cell</a></p> |
− | + | ||
− | + | ||
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− | <a href=”https://static.igem.org/mediawiki/2017/2/2f/T-NUDT_CHINA-EXPERIMENT26.PDF”>Protocol for transformation with ligation reaction system with TIANGEN DH5a competent cell</a> | + | |
Revision as of 00:35, 2 November 2017
Experiment
Protocol for CCK8 (Cell Counting kit-8)
Protocol for Lipofectamine® 2000 Transfection
Protocol for Transwell cell migration
Protocol for cell culture and subculture
Protocol for PCR with PrimeStar HS DNA polymerase
Protocol for Phosphorylation Modification
Protocol for Agarose Gel Electrophoresis
Protocol for Gel Purification with OMEGA Gel Extraction Kit
Protocol for mini-prep with OMEGA E.Z.N.A.® Plasmid DNA Mini Kit I.
Protocol for transformation with ligation reaction system with TIANGEN DH5a competent cell