Difference between revisions of "Team:Aix-Marseille/SN"

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We wanted to test the activity of [http://parts.igem.org/Part:BBa_K1491018 Supernova] on the colonies, so we made a protocol where we illuminated petri dish with a 16,5 Candela 595nm Diode (lumex ssl-lx5093syc/g) for 2 hours.
 
We wanted to test the activity of [http://parts.igem.org/Part:BBa_K1491018 Supernova] on the colonies, so we made a protocol where we illuminated petri dish with a 16,5 Candela 595nm Diode (lumex ssl-lx5093syc/g) for 2 hours.
  
−
With the following protocol :  
+
Protocol :  
  
 
*Transform 100µL TG1 with Eprc - SN (psb1C3 plasmid, 5µL)
 
*Transform 100µL TG1 with Eprc - SN (psb1C3 plasmid, 5µL)
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|3545
 
|3545
 
|3638
 
|3638
−
|}
+
|}|Aaa
  
 
The with a RFP plasmid as a control we show there's no decrease.
 
The with a RFP plasmid as a control we show there's no decrease.
  
 
With this experiment, we can conclude that supernova allows us to divide the number of colonies by 2,45.
 
With this experiment, we can conclude that supernova allows us to divide the number of colonies by 2,45.

Revision as of 00:58, 2 November 2017

SuperNova activity


We wanted to test the activity of [http://parts.igem.org/Part:BBa_K1491018 Supernova] on the colonies, so we made a protocol where we illuminated petri dish with a 16,5 Candela 595nm Diode (lumex ssl-lx5093syc/g) for 2 hours.

Protocol :

  • Transform 100µL TG1 with Eprc - SN (psb1C3 plasmid, 5µL)
  • Into 2mL LB add 2µL of 2 DO TG1 (to get an adequate number of colonies)
  • Pipet up and down
  • From the first dilution, take 8µL and dilute it into 1mL LB
  • Spin at 5000rpm for 10min
  • Remove 850µL supernatant
  • Resuspend pellet (even if you can't see it)
  • Spread remaining 150µL on petri dish
  • Immediatly put on 590nm light / put control in dark
  • After two hours, incubate overnight
  • Count colonies
Exposed Not exposed
Supernova 1496 3672
RFP 3545 3638
|Aaa

The with a RFP plasmid as a control we show there's no decrease.

With this experiment, we can conclude that supernova allows us to divide the number of colonies by 2,45.