Difference between revisions of "Team:iTesla-SoundBio/Parts"

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<p><h1>BBa_K2363000</h1>
 
<p><h1>BBa_K2363000</h1>
 
<p>-contains coding site for PCBA5 gene
 
<p>-contains coding site for PCBA5 gene
−
<p>-extracted from anaerobic organism d.mycarti
+
<p>-extracted from anaerobic organism d.mccartyi
 
<p>-resultant enzyme is a reductive dechlorinase
 
<p>-resultant enzyme is a reductive dechlorinase
 
<p>-NCBI Accession #: CP006951.1
 
<p>-NCBI Accession #: CP006951.1
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<h1>BBa_K2363001</h1>
 
<h1>BBa_K2363001</h1>
 
<p>-upstream region of PCBA4/5 gene
 
<p>-upstream region of PCBA4/5 gene
−
<p>-extracted from d.mycarti
+
<p>-extracted from d.mccartyi
 
<p>-in the future plan to characterize further
 
<p>-in the future plan to characterize further
 
<p>-160 base pairs long
 
<p>-160 base pairs long

Revision as of 01:03, 2 November 2017

BBa_K2363000

-contains coding site for PCBA5 gene

-extracted from anaerobic organism d.mccartyi

-resultant enzyme is a reductive dechlorinase

-NCBI Accession #: CP006951.1

-1442 base pairs

-no promoter or RBS

-start and stop codon added

-structural homology with pceA, same interaction with cofactor norpseudo B12

(in-depth description and sequence accesible via link below)

BBa_K2363001

-upstream region of PCBA4/5 gene

-extracted from d.mccartyi

-in the future plan to characterize further

-160 base pairs long

-used Neural Network Promoter Prediction software on BDGP to predict upstream promoter region

(in-depth description and sequence accesible via link below)

<groupparts>iGEM17 iTesla-SoundBio</groupparts>