Difference between revisions of "Team:BOKU-Vienna/Protocol"
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Revision as of 13:13, 10 July 2017
Protocol
Protocols from A-Z.
Here you will find our protocols in the future.
Materials
LB medium - E. Coli
| Name | Amount |
|---|---|
| Hy Soy Peptone | 10.0g |
| Yeast Extract | 5.0g |
| NaCl | 5.0g |
| RO-water | 1000 mL |
LB Agar - E. Coli
| Name | Amount |
|---|---|
| Hy soy Peptone | 10.0g |
| Yeast Extract | 5.0g |
| NaCl | 5.0g |
| RO-water | 980 mL |
| Agar-Agar per 500mL | 10g |
Methods
Miniprep
Minipreps were performed using Hi Yield®Plasmid Mini DNA Isolationkit according to the manufacturer's protocols.
