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<section id="works"> | <section id="works"> | ||
− | <div class="container-fluid" style="text-align: center"><div class="container"><img src="https://static.igem.org/mediawiki/2017/c/c0/BIT_figure_TrackTitle.jpeg" alt=""></div></div> | + | <div class="container-fluid" style="text-align: center"> |
− | <div class="container" style="text-align: center; padding-top: 20px"> | + | <div class="container"><img src="https://static.igem.org/mediawiki/2017/c/c0/BIT_figure_TrackTitle.jpeg" alt=""></div> |
− | + | </div> | |
− | + | <div class="container" style="text-align: center; padding-top: 20px"> | |
+ | <div style="text-align: center"> <a class="largetitle"> Stuff here are Solid #dataspeaks </a> </div> | ||
+ | <div class="container"> | ||
<div class="container" style="text-align: left; padding-top: 15px"> <a class="medtitle"> Biosensing Group </a> </div> | <div class="container" style="text-align: left; padding-top: 15px"> <a class="medtitle"> Biosensing Group </a> </div> | ||
− | <div class="container" style="text-align: left;"> <a class="bodyparagraph" style="padding-left: 30px">1. Overview</a> </div> | + | <div class="container" style="text-align: left;"> <a href="#BiosensorOverview" class="bodyparagraph" style="padding-left: 30px">1. Overview</a> </div> |
<div class="container" style="text-align: left;"> <a class="bodyparagraph" style="padding-left: 30px">2. The specific binding of AFP to AP-273</a> </div> | <div class="container" style="text-align: left;"> <a class="bodyparagraph" style="padding-left: 30px">2. The specific binding of AFP to AP-273</a> </div> | ||
<div class="container" style="text-align: left;"> <a class="bodyparagraph" style="padding-left: 30px">3. EDC Plus Sulfo-NHS</a> </div> | <div class="container" style="text-align: left;"> <a class="bodyparagraph" style="padding-left: 30px">3. EDC Plus Sulfo-NHS</a> </div> | ||
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<div class="container" style="text-align: left;"> <a class="bodyparagraph" style="padding-left: 30px">6. Enzyme Linked: To link the target gene to the vector.</a> </div> | <div class="container" style="text-align: left;"> <a class="bodyparagraph" style="padding-left: 30px">6. Enzyme Linked: To link the target gene to the vector.</a> </div> | ||
<div class="container" style="text-align: left;"> <a class="bodyparagraph" style="padding-left: 30px">7. Electrophoresis.</a> </div> | <div class="container" style="text-align: left;"> <a class="bodyparagraph" style="padding-left: 30px">7. Electrophoresis.</a> </div> | ||
− | + | <div class="container" style="text-align: left; padding-top: 15px"> <a class="medtitle"> Enzyme Digestion and Processing </a> </div> | |
<div class="container" style="text-align: left;"> <a class="bodyparagraph" style="padding-left: 30px">1. Electroporation</a> </div> | <div class="container" style="text-align: left;"> <a class="bodyparagraph" style="padding-left: 30px">1. Electroporation</a> </div> | ||
<div class="container" style="text-align: left;"> <a class="bodyparagraph" style="padding-left: 30px">2. Knock-Out of LysA Gene in E.coli BW25113 by λRed Recombination System</a> </div> | <div class="container" style="text-align: left;"> <a class="bodyparagraph" style="padding-left: 30px">2. Knock-Out of LysA Gene in E.coli BW25113 by λRed Recombination System</a> </div> | ||
− | <div class="container" style="text-align: left;"> <a class="bodyparagraph" style="padding-left: 30px">3. The Preparation of Targeting Vectorm</a> </div> | + | <div class="container" style="text-align: left;"> <a class="bodyparagraph" style="padding-left: 30px">3. The Preparation of Targeting Vectorm</a> </div> |
− | + | <div class="container" style="text-align: left;"> <a class="bodyparagraph" style="padding-left: 30px">4. The Transformation of Plasmid pKD46</a> </div> | |
− | + | <div class="container" style="text-align: left;"> <a class="bodyparagraph" style="padding-left: 30px">5. The Verification of Strain</a> </div> | |
− | + | <div class="container" style="text-align: left;"> <a class="bodyparagraph" style="padding-left: 60px">A. Antibiotics Preparation</a> </div> | |
− | + | <div class="container" style="text-align: left;"> <a class="bodyparagraph" style="padding-left: 60px">B. Competent Cell Preparation</a> </div> | |
− | + | <div class="container" style="text-align: left;"> <a class="bodyparagraph" style="padding-left: 60px">C. Submission DNA Preparation</a> </div> | |
− | + | <div class="container" style="text-align: left;"> <a class="bodyparagraph" style="padding-left: 60px">D. TIANamp Bacteria DNA Kit</a> </div> | |
+ | </div> | ||
+ | </div> | ||
+ | <div class="container" style="text-align: center; padding-top: 50px"> | ||
+ | <a name="BiosensorOverview"></a> | ||
+ | <embed src="https://static.igem.org/mediawiki/2017/b/b6/IGEM_BIT_Protocol_lay_17.pdf" width="600" height="800" type="application/pdf"> | ||
</div> | </div> | ||
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</section> | </section> | ||
<!-- portfolio section --> | <!-- portfolio section --> |
Revision as of 01:40, 2 November 2017