Difference between revisions of "Team:BOKU-Vienna/Notebookdata"
| Line 13: | Line 13: | ||
</ul> | </ul> | ||
<li>Planning of the procedures including primer design</li> | <li>Planning of the procedures including primer design</li> | ||
| − | <li>Preparing the growth media and autoclaving | + | <li><a href="https://2017.igem.org/Team:BOKU-Vienna/Protocol#lbmedium1" target="_blank">Preparing the growth media and autoclaving</a></li> |
<ul><li>LB medium preparation (4L): Mina, Michael</li></ul> | <ul><li>LB medium preparation (4L): Mina, Michael</li></ul> | ||
<ul><li>Autoclaving: Mina, Rebecca</li></ul> | <ul><li>Autoclaving: Mina, Rebecca</li></ul> | ||
| Line 27: | Line 27: | ||
</ul> | </ul> | ||
<li>Preparation of growth media: [LINK ZUM PROTOKOLL]</li> | <li>Preparation of growth media: [LINK ZUM PROTOKOLL]</li> | ||
| − | <ul><li>20 plates LB medium + chloramphenicol: Mina, Lisa, Mathias</li></ul> | + | <ul><li>20 plates <a href="https://2017.igem.org/Team:BOKU-Vienna/Protocol#lbmedium1" target="_blank">LB medium</a> + chloramphenicol: Mina, Lisa, Mathias</li></ul> |
| − | <ul><li>2 flasks LB agar: Mina, Lisa, Mathias</li></ul> | + | <ul><li>2 flasks <a href="https://2017.igem.org/Team:BOKU-Vienna/Protocol#lbagar" target="_blank">LB agar</a>: Mina, Lisa, Mathias</li></ul> |
</ul> | </ul> | ||
</div> | </div> | ||
| Line 43: | Line 43: | ||
<li>Transferring Backbones onto agar plates:</li> | <li>Transferring Backbones onto agar plates:</li> | ||
<ul><li>Julian</li></ul> | <ul><li>Julian</li></ul> | ||
| − | <li>Interlab challenge - transformation: According to | + | <li>Interlab challenge - transformation: According to <a href="https://2017.igem.org/Team:BOKU-Vienna/Protocol#transformation" target="_blank">protocol</a>:</li> |
<ul>Lisa, Mathias, Michael, Mina, Rebecca</ul> | <ul>Lisa, Mathias, Michael, Mina, Rebecca</ul> | ||
| − | <ul><li>8 transformations - from Kit Plate 6 InterLab | + | <ul><li>8 transformations - from <a href="https://2017.igem.org/Team:BOKU-Vienna/Protocol#interlab" target="_blank">Kit Plate 6 InterLab</a></li></ul> |
<ul><li>8 plasmids plated onto agar medium: Positive control (50µL, 200µL), Negative Control (50µL, 200µL), Transformant (20µL, 100µL, 200µL), Test device 1-6 (50µL, 200µL)</li></ul> | <ul><li>8 plasmids plated onto agar medium: Positive control (50µL, 200µL), Negative Control (50µL, 200µL), Transformant (20µL, 100µL, 200µL), Test device 1-6 (50µL, 200µL)</li></ul> | ||
<ul><li>stored at 35°C</li></ul> | <ul><li>stored at 35°C</li></ul> | ||
| Line 57: | Line 57: | ||
<li>Counting colonies of Transformants for the Interlab Challenge: [HIER LINK ZU ERGEBNISSEN]</li> | <li>Counting colonies of Transformants for the Interlab Challenge: [HIER LINK ZU ERGEBNISSEN]</li> | ||
<ul><li>Colonies 200µL: 77, 20µL: 26, 100µL: 43</li></ul> | <ul><li>Colonies 200µL: 77, 20µL: 26, 100µL: 43</li></ul> | ||
| − | <li>Miniprep of 8 plasmids for Golden Gate Standard - according to protocol | + | <li>Miniprep of 8 plasmids for Golden Gate Standard - according to <a href="https://2017.igem.org/Team:BOKU-Vienna/Protocol#miniprep" target="_blank">protocol</a></li> |
<ul><li>Magdalena (1), Melanie (2), Valentin (3), Rebeccca (4), Christina (5), Martin (6), Mina (7), Lisa (8)</li></ul> | <ul><li>Magdalena (1), Melanie (2), Valentin (3), Rebeccca (4), Christina (5), Martin (6), Mina (7), Lisa (8)</li></ul> | ||
<ul><li>Dilution of plasmids: Lisa [LINK ZU DILUTION SHEET]</li></ul> | <ul><li>Dilution of plasmids: Lisa [LINK ZU DILUTION SHEET]</li></ul> | ||
| Line 67: | Line 67: | ||
<ul> | <ul> | ||
<li>Team discussion, Planning and Fundraising</li> | <li>Team discussion, Planning and Fundraising</li> | ||
| − | <li>Interlab Challenge: Fluorescein fluorescence standard curve</li> | + | <li><a href="https://2017.igem.org/Team:BOKU-Vienna/Protocol#interlab" target="_blank">Interlab Challenge</a>: Fluorescein fluorescence standard curve</li> |
<ul><li>Christina</li></ul> | <ul><li>Christina</li></ul> | ||
</ul> | </ul> | ||
Revision as of 15:18, 10 July 2017
Monday, July 3rd.
- Team discussion and overview:
- Christina, Felicia, Josef, Lisa, Magdalena, Mathias, Melanie, Michael, Mina, Rebecca, Valentin, Julian
- Planning of the procedures including primer design
- Preparing the growth media and autoclaving
- LB medium preparation (4L): Mina, Michael
- Autoclaving: Mina, Rebecca
Tuesday, July 4th.
Wednesday, July 5th.
- Planning and fundraising:
- Christina, Felicia, Josef, Lisa, Magdalena, Martin, Mathias, Melanie, Michael, Mina, Rebecca, Julian
- Aliquoting 1mL LB into Eppendorf tubes
- Lisa, Josef
- Transferring Backbones onto agar plates:
- Julian
- Interlab challenge - transformation: According to protocol:
- 8 transformations - from Kit Plate 6 InterLab
- 8 plasmids plated onto agar medium: Positive control (50µL, 200µL), Negative Control (50µL, 200µL), Transformant (20µL, 100µL, 200µL), Test device 1-6 (50µL, 200µL)
- stored at 35°C
- Lisa, Mathias, Michael, Mina, Rebecca
Thursday, July 6th.
- Team discussion, planning and fundraising
- Counting colonies of Transformants for the Interlab Challenge: [HIER LINK ZU ERGEBNISSEN]
- Colonies 200µL: 77, 20µL: 26, 100µL: 43
- Miniprep of 8 plasmids for Golden Gate Standard - according to protocol
- Magdalena (1), Melanie (2), Valentin (3), Rebeccca (4), Christina (5), Martin (6), Mina (7), Lisa (8)
- Dilution of plasmids: Lisa [LINK ZU DILUTION SHEET]
Friday, July 7th.
- Team discussion, Planning and Fundraising
- Interlab Challenge: Fluorescein fluorescence standard curve
- Christina
