Difference between revisions of "Team:BOKU-Vienna/Notebookdata"

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         <li>Christina, Felicia, Josef, Lisa, Martin, Mathias, Melanie, Michael, Mina, Rebecca, Valentin, Julian</li>
 
         <li>Christina, Felicia, Josef, Lisa, Martin, Mathias, Melanie, Michael, Mina, Rebecca, Valentin, Julian</li>
 
       </ul>
 
       </ul>
−
     <li>Preparation of growth media: [LINK ZUM PROTOKOLL]</li>
+
     <li>Preparation of growth media:</li>
 
       <ul><li>20 plates <a href="https://2017.igem.org/Team:BOKU-Vienna/Protocol#lbmedium1" target="_blank">LB medium</a> + chloramphenicol: Mina, Lisa, Mathias</li></ul>
 
       <ul><li>20 plates <a href="https://2017.igem.org/Team:BOKU-Vienna/Protocol#lbmedium1" target="_blank">LB medium</a> + chloramphenicol: Mina, Lisa, Mathias</li></ul>
 
       <ul><li>2 flasks <a href="https://2017.igem.org/Team:BOKU-Vienna/Protocol#lbagar" target="_blank">LB agar</a>: Mina, Lisa, Mathias</li></ul>
 
       <ul><li>2 flasks <a href="https://2017.igem.org/Team:BOKU-Vienna/Protocol#lbagar" target="_blank">LB agar</a>: Mina, Lisa, Mathias</li></ul>

Revision as of 15:19, 10 July 2017

Monday, July 3rd.

  • Team discussion and overview:
    • Christina, Felicia, Josef, Lisa, Magdalena, Mathias, Melanie, Michael, Mina, Rebecca, Valentin, Julian
  • Planning of the procedures including primer design
  • Preparing the growth media and autoclaving
    • LB medium preparation (4L): Mina, Michael
    • Autoclaving: Mina, Rebecca

Tuesday, July 4th.

  • Planning and fundraising:
    • Christina, Felicia, Josef, Lisa, Martin, Mathias, Melanie, Michael, Mina, Rebecca, Valentin, Julian
  • Preparation of growth media:
    • 20 plates LB medium + chloramphenicol: Mina, Lisa, Mathias
    • 2 flasks LB agar: Mina, Lisa, Mathias

Wednesday, July 5th.

  • Planning and fundraising:
    • Christina, Felicia, Josef, Lisa, Magdalena, Martin, Mathias, Melanie, Michael, Mina, Rebecca, Julian
  • Aliquoting 1mL LB into Eppendorf tubes
    • Lisa, Josef
  • Transferring Backbones onto agar plates:
    • Julian
  • Interlab challenge - transformation: According to protocol:
      • Lisa, Mathias, Michael, Mina, Rebecca
    • 8 plasmids plated onto agar medium: Positive control (50µL, 200µL), Negative Control (50µL, 200µL), Transformant (20µL, 100µL, 200µL), Test device 1-6 (50µL, 200µL)
    • stored at 35°C

Thursday, July 6th.

  • Team discussion, planning and fundraising
  • Counting colonies of Transformants for the Interlab Challenge: [HIER LINK ZU ERGEBNISSEN]
    • Colonies 200µL: 77, 20µL: 26, 100µL: 43
  • Miniprep of 8 plasmids for Golden Gate Standard - according to protocol
    • Magdalena (1), Melanie (2), Valentin (3), Rebeccca (4), Christina (5), Martin (6), Mina (7), Lisa (8)
    • Dilution of plasmids: Lisa [LINK ZU DILUTION SHEET]

Friday, July 7th.

  • Team discussion, Planning and Fundraising
  • Interlab Challenge: Fluorescein fluorescence standard curve
    • Christina