Difference between revisions of "Team:BOKU-Vienna/Notebookdata"

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     <li>Miniprep of 8 plasmids for Golden Gate Standard - according to <a href="https://2017.igem.org/Team:BOKU-Vienna/Protocol#miniprep" target="_blank">protocol</a></li>
 
     <li>Miniprep of 8 plasmids for Golden Gate Standard - according to <a href="https://2017.igem.org/Team:BOKU-Vienna/Protocol#miniprep" target="_blank">protocol</a></li>
 
       <ul><li>Magdalena (1), Melanie (2), Valentin (3), Rebeccca (4), Christina (5), Martin (6), Mina (7), Lisa (8)</li></ul>
 
       <ul><li>Magdalena (1), Melanie (2), Valentin (3), Rebeccca (4), Christina (5), Martin (6), Mina (7), Lisa (8)</li></ul>
−
       <ul><li>Dilution of plasmids: Lisa [LINK ZU DILUTION SHEET]</li></ul>
+
       <ul><li>Dilution of plasmids for Golden Gate: Lisa</li></ul>
 
   </ul>
 
   </ul>
 +
 +
<h5 id="dilution">Dilution sheet for Golden Gate Assembly</h5>
 +
<p></p>
 +
<table class="table">
 +
<thead>
 +
<tr>
 +
<th>Sample</th>
 +
                <th>Volume of AD - RO Water [µL]</th>
 +
<th>Volume of plasmid/fragment [µL]</th>
 +
</tr>
 +
</thead>
 +
<tbody>
 +
<tr>
 +
<td>BB1-C-23-Spy-Cas9(NLS)-49</td>
 +
<td>71,6</td>
 +
                <td>28,4</td>
 +
</tr>
 +
<tr>
 +
<td>BB1-T-34-sce-cyc-50</td>
 +
<td>83,0</td>
 +
                <td>17,0</td>
 +
</tr>
 +
        <tr>
 +
<td>BB1-P-12-syr-pNS-J23101-25</td>
 +
<td>85,1</td>
 +
                <td>14,9</td>
 +
</tr>
 +
        <tr>
 +
<td>BB1-P-12-sce-tdh3-45</td>
 +
<td>82,7</td>
 +
                <td>17,3</td>
 +
</tr>
 +
        <tr>
 +
<td>BB1-T-34-eco-B1001-29</td>
 +
<td>80,4</td>
 +
                <td>19,6</td>
 +
</tr>
 +
        <tr>
 +
<td>BB1-P-12-syn-pm-J23105-23</td>
 +
<td>83,9</td>
 +
                <td>16,1</td>
 +
</tr>
 +
        <tr>
 +
<td>BB1-C-23-syn-GFP-30</td>
 +
<td>82,9</td>
 +
                <td>17,1</td>
 +
</tr>
 +
        <tr>
 +
<td>BB1-P-12-sce-tef1-46</td>
 +
<td>78,0</td>
 +
                <td>22,0</td>
 +
</tr>
 +
</tbody>
 +
</table>
 
</div>
 
</div>
  

Revision as of 15:52, 10 July 2017

Monday, July 3rd.

  • Team discussion and overview:
    • Christina, Felicia, Josef, Lisa, Magdalena, Mathias, Melanie, Michael, Mina, Rebecca, Valentin, Julian
  • Planning of the procedures including primer design
  • Preparing the growth media and autoclaving
    • LB medium preparation (4L): Mina, Michael
    • Autoclaving: Mina, Rebecca

Tuesday, July 4th.

  • Planning and fundraising:
    • Christina, Felicia, Josef, Lisa, Martin, Mathias, Melanie, Michael, Mina, Rebecca, Valentin, Julian
  • Preparation of growth media:
    • 20 plates LB medium + chloramphenicol: Mina, Lisa, Mathias
    • 2 flasks LB agar: Mina, Lisa, Mathias

Wednesday, July 5th.

  • Planning and fundraising:
    • Christina, Felicia, Josef, Lisa, Magdalena, Martin, Mathias, Melanie, Michael, Mina, Rebecca, Julian
  • Aliquoting 1mL LB into Eppendorf tubes
    • Lisa, Josef
  • Transferring Backbones onto agar plates:
    • Julian
  • Interlab challenge - transformation: According to protocol:
      • Lisa, Mathias, Michael, Mina, Rebecca
    • 8 plasmids plated onto agar medium: Positive control (50µL, 200µL), Negative Control (50µL, 200µL), Transformant (20µL, 100µL, 200µL), Test device 1-6 (50µL, 200µL)
    • stored at 35°C

Thursday, July 6th.

  • Team discussion, planning and fundraising
  • Counting colonies of Transformants for the Interlab Challenge:
    • Colonies 200µL: 77, 20µL: 26, 100µL: 43
    Transformants on plate Transformants on plate Transformants on plate
  • Miniprep of 8 plasmids for Golden Gate Standard - according to protocol
    • Magdalena (1), Melanie (2), Valentin (3), Rebeccca (4), Christina (5), Martin (6), Mina (7), Lisa (8)
    • Dilution of plasmids for Golden Gate: Lisa
Dilution sheet for Golden Gate Assembly

Sample Volume of AD - RO Water [µL] Volume of plasmid/fragment [µL]
BB1-C-23-Spy-Cas9(NLS)-49 71,6 28,4
BB1-T-34-sce-cyc-50 83,0 17,0
BB1-P-12-syr-pNS-J23101-25 85,1 14,9
BB1-P-12-sce-tdh3-45 82,7 17,3
BB1-T-34-eco-B1001-29 80,4 19,6
BB1-P-12-syn-pm-J23105-23 83,9 16,1
BB1-C-23-syn-GFP-30 82,9 17,1
BB1-P-12-sce-tef1-46 78,0 22,0

Friday, July 7th.

  • Team discussion, Planning and Fundraising
  • Interlab Challenge: Fluorescein fluorescence standard curve
    • Christina