Difference between revisions of "Team:UNC-Asheville/CompositeParts"
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<p>dhlB2 is a haloalkanoic acid dehalogenase isolated from the Xantobacter autotrophicus strain GJ10. It is active on a number of halogenated alkanes including chloroacetic acid and dichloroacetic acid. This basic part was characterized by the SydneyUni_2013 iGEM team but the part sequenced poorly and was never made a biobrick. The UNCA_2017 iGEM team had this part synthesized by Genscript after including a double promoter region (Anderson weak + lac, BBa_J23114 BBa_R0011) and a double terminator region (B0010, B0012) </p></blockquote> | <p>dhlB2 is a haloalkanoic acid dehalogenase isolated from the Xantobacter autotrophicus strain GJ10. It is active on a number of halogenated alkanes including chloroacetic acid and dichloroacetic acid. This basic part was characterized by the SydneyUni_2013 iGEM team but the part sequenced poorly and was never made a biobrick. The UNCA_2017 iGEM team had this part synthesized by Genscript after including a double promoter region (Anderson weak + lac, BBa_J23114 BBa_R0011) and a double terminator region (B0010, B0012) </p></blockquote> | ||
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| + | <div class="container"> | ||
| + | <div class="row center header"> | ||
| + | <h1 class ="title">soluble Methane Monooxygenase<br><small>sMMO</small></h1> | ||
| + | </div> | ||
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| + | <div class="container-fluid"> | ||
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| + | <div class="col-sm-offset-1 col-sm-5"> | ||
| + | <blockquote> | ||
| + | <br> | ||
| + | <h1>sMMO_1</h1> | ||
| + | <p>soluble methane monooxygenase is a strong oxidizing protein that catalyzes methane to methanol in methanophile bacteria. The biobrick BBa_K1390019 was a complete composite part of sMMO that included all 6 subunits downstream of weak RBS (B0032). The part was lac inducible via BBa_R0011 and included a double terminator region with B0010 and B0012. | ||
| + | Unfortunately, this biobrick had a bad read and was never confirmed as a part. Our team attempted to create this biobrick once and for all. sMMO1 is the first half and includes the promoter region, plus the coding regions of mmoX and mmoY. | ||
| + | </p></blockquote> | ||
| + | </div> | ||
| + | <div class="col-sm-offset-1 col-sm-4"><img src="https://static.igem.org/mediawiki/2017/8/8e/UNCA-GroGel.png" class="img-fluid well" alt="Responsive image"></div> | ||
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Revision as of 02:23, 2 November 2017
Composite Parts
Standing on the shoulders of giants.
dhlB
dhlB2_Lac_Inducible
Description
dhlB2 is a haloalkanoic acid dehalogenase isolated from the Xantobacter autotrophicus strain GJ10. It is active on a number of halogenated alkanes including chloroacetic acid and dichloroacetic acid. This basic part was characterized by the SydneyUni_2013 iGEM team but the part sequenced poorly and was never made a biobrick. The UNCA_2017 iGEM team had this part synthesized by Genscript after including a double promoter region (Anderson weak + lac, BBa_J23114 BBa_R0011) and a double terminator region (B0010, B0012)
soluble Methane Monooxygenase
sMMO
sMMO_1
soluble methane monooxygenase is a strong oxidizing protein that catalyzes methane to methanol in methanophile bacteria. The biobrick BBa_K1390019 was a complete composite part of sMMO that included all 6 subunits downstream of weak RBS (B0032). The part was lac inducible via BBa_R0011 and included a double terminator region with B0010 and B0012. Unfortunately, this biobrick had a bad read and was never confirmed as a part. Our team attempted to create this biobrick once and for all. sMMO1 is the first half and includes the promoter region, plus the coding regions of mmoX and mmoY.
