Difference between revisions of "Team:UNBC-Canada/Demonstrate"

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<h1>Demonstrate</h1>
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<h1>Demonstrate - Fluorescence Polarization</h1>
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<h3>Gold Medal Criterion #4</h3>
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Teams that can show their system working under real world conditions are usually good at impressing the judges in iGEM. To achieve gold medal criterion #4, convince the judges that your project works. There are many ways in which your project working could be demonstrated, so there is more than one way to meet this requirement. This gold medal criterion was introduced in 2016, so check our what 2016 teams did to achieve a their gold medals!
 
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Please see the <a href="https://2017.igem.org/Judging/Medals">2017 Medals Page</a> for more information.
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We set out to determine whether or not protein Hfq is required for sRNA-mRNA hybridization in Staphylococcus aureus. Our FP assay results suggest that Hfq does not bind to the sRNA binding region we synthesized based on results from the literature, and therefore does not effect sRNA-mRNA hybridization in Staphylococcus aureus. When the protein concentration was then increased 100 fold and tested with only the AUAUAUA oligo reported in the literature to bind strongly to Hfq, with no significant difference between wells without protein. Therefore, we concluded that sRNA mediated gene silencing is possible in Staphylococcus species without the inclusion of Hfq on the vector delivered to target cells.
 
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<h4> What should we do for our demonstration?</h4>
 
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<h5> Standard teams </h5>
 
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If you have built a proof of concept system, you can demonstrate it working under real world conditions. If you have built a biological device that is intended to be a sensor, can you show it detecting whatever it is intended to sense. If it is intended to work in the field, you can show how this might work using a simulated version in the lab, or a simulation of your device in the field.<strong> Please note biological materials must not be taken out of the lab</strong>.
 
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<h5> Special track teams </h5>
 
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Special track teams can achieve this medal criterion by bringing their work to the Jamboree and showcasing it in the track event. Art & Design, Measurement, Hardware and Software tracks will all have showcase events at the Giant Jamboree.<strong> Please note biological materials must not be taken out of the lab</strong>.
 
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Revision as of 02:35, 2 November 2017

Demonstrate - Fluorescence Polarization

We set out to determine whether or not protein Hfq is required for sRNA-mRNA hybridization in Staphylococcus aureus. Our FP assay results suggest that Hfq does not bind to the sRNA binding region we synthesized based on results from the literature, and therefore does not effect sRNA-mRNA hybridization in Staphylococcus aureus. When the protein concentration was then increased 100 fold and tested with only the AUAUAUA oligo reported in the literature to bind strongly to Hfq, with no significant difference between wells without protein. Therefore, we concluded that sRNA mediated gene silencing is possible in Staphylococcus species without the inclusion of Hfq on the vector delivered to target cells.