Difference between revisions of "Team:BGIC-Union/InterLab"

 
Line 1: Line 1:
  
 
<html>
 
<html>
 +
  <head>
 +
  <html>
 
   <head>
 
   <head>
 
     <meta charset="utf-8">
 
     <meta charset="utf-8">
Line 11: Line 13:
 
   <head>
 
   <head>
 
    
 
    
−
  <!-- main -->
+
 
 +
  <!-- Bootstrap core CSS -->
 +
    <link href="https://2017.igem.org/Template:BGIC-Union/bootstrap-css?action=raw&amp;ctype=text/css" rel="stylesheet">
 +
<!-- animate -->
 +
<link href="https://2017.igem.org/Template:BGIC-Union/animate-css?action=raw&amp;ctype=text/css" rel="stylesheet">
 +
  <!-- carousel -->
 +
  <link href="https://2017.igem.org/Template:BGIC-Union/owlcarousel-css?action=raw&amp;ctype=text/css" rel="stylesheet">
 +
  <link href=" https://2017.igem.org/Template:BGIC-Union/fontawesomemin2-css?action=raw&amp;ctype=text/css" rel="stylesheet">
 +
  <!-- light -->
 +
<style type="text/css">
 +
/* fixed line holder */
 +
.timelineLight .timeline_line {c
 +
margin-top:50px;
 +
margin-bottom:10px;
 +
width:900px;
 +
}
 +
.timelineLight
 +
{color: #fff;
 +
background: #ffe4e1;}
 +
/* full (including months that are not shown) line holder */
 +
.timelineLight .t_line_holder {
 +
height:80px;
 +
background:url('https://static.igem.org/mediawiki/2017/3/35/Line.jpg') repeat-x 0px 39px;
 +
}
 +
 
 +
/* 2 months are stored in one view */
 +
.timelineLight .t_line_view {
 +
height:20px;
 +
width:900px;
 +
}
 +
 
 +
/* holder for year number */
 +
.timelineLight h3.t_line_year {
 +
margin:0;
 +
color:#545454;
 +
}
 +
 
 +
/* holder for 1 month (constist of nodes and month caption) - we use borders to separate months thats why it has width 2px less then 50% */
 +
.timelineLight .t_line_m {
 +
margin-top:35px;
 +
height:10px;
 +
border-left:1px solid #545454;
 +
border-right:1px solid #545454;
 +
width:448px;
 +
}
 +
 
 +
/* month on the right side - has left set at 459 so border would overlap border from first element (to evade duplicated borders) */
 +
.timelineLight .t_line_m.right {
 +
left:449px;
 +
width:449px;
 +
}
 +
 
 +
/* month caption */
 +
.timelineLight h4.t_line_month {
 +
margin:-30px 0 0;
 +
color:#545454;
 +
}
 +
 
 +
/* used in responsive layout when only one month is shown (it is span containing year) */
 +
.t_line_month_year {
 +
display:none;
 +
}
 +
 
 +
/* node on the timeline */
 +
.timelineLight a.t_line_node {
 +
text-decoration:none;
 +
padding:38px 0 4px;
 +
height:10px;
 +
font-size:12px;
 +
top:-25px;
 +
background:url('https://static.igem.org/mediawiki/2017/2/2a/Dot.png') no-repeat center 24px;
 +
color:#141817;
 +
}
 +
.timelineLight a.t_line_node:hover {
 +
background:url('https://static.igem.org/mediawiki/2017/6/65/Dot-rollover.png') no-repeat center 24px;
 +
}
 +
.timelineLight a.t_line_node.active {
 +
background:url('https://static.igem.org/mediawiki/2017/d/d7/Dot-selected.png') no-repeat center 24px;
 +
}
 +
 
 +
/* node description */
 +
.timelineLight .t_node_desc {
 +
background: rgba(26,134,172,0.9);
 +
top:0;
 +
color:#fff;
 +
padding:1px 5px;
 +
}
 +
 
 +
/* descriptions on right side go from right to left */
 +
.timelineLight .t_node_desc.pos_right {
 +
right:0;
 +
}
 +
 
 +
/* line arrow left */
 +
.timelineLight #t_line_left {
 +
cursor:pointer;
 +
left:-30px;
 +
top:30px;
 +
width:14px;
 +
height:19px;
 +
background:url('https://static.igem.org/mediawiki/2017/9/95/Bgic-arrow.png') no-repeat left top;
 +
}
 +
 
 +
.timelineLight #t_line_left:hover {
 +
background:url('https://static.igem.org/mediawiki/2017/9/95/Bgic-arrow.png') no-repeat left bottom;
 +
}
 +
 
 +
/* line arrow right */
 +
.timelineLight #t_line_right {
 +
cursor:pointer;
 +
right:-30px;
 +
top:30px;
 +
width:14px;
 +
height:19px;
 +
background:url('https://static.igem.org/mediawiki/2017/9/95/Bgic-arrow.png') no-repeat right top;
 +
}
 +
 
 +
.timelineLight #t_line_right:hover {
 +
background:url('https://static.igem.org/mediawiki/2017/9/95/Bgic-arrow.png') no-repeat right bottom;
 +
}
 +
.timelineLight {
 +
display:none;
 +
}
 +
 
 +
/* items container */
 +
.timelineLight .timeline_items {
 +
padding:10px 0;
 +
}
 +
 
 +
/* single item (margines set from javascript) */
 +
.timelineLight .item {
 +
height:380px;
 +
text-align:center;
 +
 +
color: #fff;
 +
background: #f7dae7;
 +
 +
-moz-box-shadow: -3px 1px 6px rgba(0,0,0,0.4);
 +
-webkit-box-shadow: -3px 1px 6px rgba(0,0,0,0.4);
 +
box-shadow: -3px 1px 6px rgba(0,0,0,0.4);
 +
width:200px;
 +
}
 +
 
 +
/* ----- content - non-plugin elements ----- */
 +
.timelineLight .item img class="center"{
 +
margin-top:0;
 +
width:200px;
 +
}
 +
 
 +
.timelineLight .item span{
 +
display:block;
 +
margin:0px 20px 10px;
 +
height:150px;
 +
}
 +
.timelineLight .item .read_more {
 +
padding:2px 8px 2px 10px;
 +
font-family:Comic Sans MS;
 +
font-size:20px;
 +
float:right;
 +
color:#ffffff;
 +
background: rgba(0,0,0,0.35);
 +
cursor:pointer;
 +
}
 +
.timelineLight .item .read_more:hover {
 +
background:rgb(26,134,172);
 +
}
 +
/* ----------------------------------------- */
 +
 
 +
/* item details (margines set from javascript) */
 +
.timelineLight .item_open {
 +
height:380px;
 +
background:url('../images/timeline/light/background.jpg') repeat;
 +
position:relative;
 +
color:#545454;
 +
z-index:2;
 +
-moz-box-shadow: 0px 0px 6px rgba(0,0,0,0.4);
 +
-webkit-box-shadow: 0px 0px 6px rgba(0,0,0,0.4);
 +
box-shadow: 0px 0px 6px rgba(0,0,0,0.4);
 +
width:490px;
 +
 +
}
 +
 
 +
/* item details content wrapper (used for animation - shuld have same width as .item_open) */
 +
.timelineLight .item_open_cwrapper {
 +
width:490px;
 +
}
 +
 
 +
/* ----- content - non-plugin elements ----- */
 +
.timelineLight .timeline_open_content {
 +
padding:20px;
 +
}
 +
 
 +
.timelineLight .item_open h2 {
 +
margin-top:10px;
 +
padding-top:0;
 +
font-size:28px;
 +
}
 +
.timelineLight .item_open .t_close {
 +
position:absolute;
 +
top:10px;
 +
right:10px;
 +
padding:2px 8px 2px 10px;
 +
font-family: Comic Sans MS;
 +
font-size:20px;
 +
color:#ffffff;
 +
background: rgba(0,0,0,0.25);
 +
cursor:pointer;
 +
}
 +
.timelineLight .item_open .t_close:hover {
 +
background:rgb(26,134,172);
 +
}
 +
/* ----------------------------------------- */
 +
 
 +
/* left/right controles */
 +
.timelineLight .t_controles {
 +
margin:10px auto;
 +
text-align:center;
 +
}
 +
.timelineLight .t_left,
 +
.timelineLight .t_right {
 +
display:inline-block;
 +
height:50px;
 +
width:29px;
 +
margin:10px;
 +
cursor:pointer;
 +
}
 +
.timelineLight .t_left,  
 +
.timelineLight .t_left:hover:active {
 +
background: url('https://static.igem.org/mediawiki/2017/b/ba/Big-arrow.png') no-repeat left top;
 +
}
 +
.timelineLight .t_left:hover {
 +
background: url('https://static.igem.org/mediawiki/2017/b/ba/Big-arrow.png') no-repeat left bottom;
 +
}
 +
 
 +
.timelineLight .t_right,
 +
.timelineLight .t_right:hover:active{
 +
background: url('https://static.igem.org/mediawiki/2017/b/ba/Big-arrow.png') no-repeat right top;
 +
}
 +
 
 +
.timelineLight .t_right:hover {
 +
background: url('https://static.igem.org/mediawiki/2017/b/ba/Big-arrow.png') no-repeat right bottom;
 +
}
 +
 
 +
/* -----------------------------------------------------------------------*/
 +
/* ------------------------------ RESPONSIVE -----------------------------*/
 +
/* -----------------------------------------------------------------------*/
 +
 
 +
 
 +
 
 +
/* --- 768px --- */
 +
@media screen and (max-width:980px) {
 +
 +
.timelineLight .timeline_line {
 +
width:680px;
 +
}
 +
 +
.timelineLight .t_line_view {
 +
width:680px;
 +
}
 +
 +
.timelineLight .t_line_m {
 +
width: 338px;
 +
}
 +
.timelineLight .t_line_m.right {
 +
left: 339px;
 +
width: 339px;
 +
}
 +
}
 +
 
 +
 
 +
/* --- 610px --- */
 +
@media screen and (max-width:767px) {
 +
 +
.timelineLight .timeline_line {
 +
width:530px;
 +
}
 +
 +
.timelineLight .t_line_view {
 +
width:1060px;
 +
}
 +
 +
.timelineLight .t_line_m {
 +
width: 528px;
 +
}
 +
.timelineLight .t_line_m.right {
 +
left: 530px;
 +
width: 528px;
 +
}
 +
.timelineLight .t_line_year {
 +
opacity:0;
 +
filter:alpha(opacity=0)
 +
}
 +
.timelineLight .t_line_month_year {
 +
display:inline;
 +
}
 +
 +
.timelineLight .t_line_node span {
 +
 +
}
 +
.timelineLight .t_node_desc {
 +
font-size:8px;
 +
}
 +
.timelineLight .t_node_desc.pos_right {
 +
right:auto;
 +
left:0;
 +
}
 +
}
 +
 
 +
 
 +
/* --- 300px --- */
 +
@media screen and (max-width:
 +
 
 +
) {
 +
.timelineLight .timeline_line {
 +
width:240px;
 +
}
 +
 +
.timelineLight .t_line_view {
 +
width:480px;
 +
}
 +
 +
.timelineLight .t_line_m {
 +
width: 238px;
 +
}
 +
.timelineLight .t_line_m.right {
 +
left: 240px;
 +
width: 238px;
 +
}
 +
 +
.timelineLight .item_open {
 +
width:280px;
 +
height:390px;
 +
}
 +
.timelineLight .item_open_cwrapper {
 +
width:280px;
 +
}
 +
}
 +
 
 +
 
 +
 
 +
 
 +
</style>
 +
 
 +
  <!-- main -->
 
   <style type="text/css">
 
   <style type="text/css">
 
    
 
    
−
@import url(http://fonts.googleapis.com/css?family=Montserrat:400,700);
+
 
 
#top_title,#sideMenu { display: none !important; }
 
#top_title,#sideMenu { display: none !important; }
 
         #content {
 
         #content {
Line 23: Line 368:
 
         }
 
         }
  
 +
#bodyContent
 +
{
 +
margin-top：0px !important;
 +
}
  
−
#footer{
 
−
padding-botton:-60px;
 
−
}
 
  
 
#content{
 
#content{
Line 33: Line 379:
  
  
−
+
.mainstyle
 +
{
 +
font-family: Comic Sans MS;
 +
}
 
html, body {
 
html, body {
−
     margin: 0 !important;
+
     margin: 0 ;
−
     padding: 0 !important;
+
     padding: 0 ;
−
 
+
 
}
 
}
 
body {
 
body {
−
   background-color: #FFe4e1;
+
   background-color: #ffa07a;
−
   font-family: 'Montserrat', sans-serif;
+
  
 
     font-weight: 400;
 
     font-weight: 400;
−
line-height: 45px;
+
     font-size: 14px;
−
     font-size: 20px;
+
 
     color: #555;
 
     color: #555;
−
 
+
     ；
−
      
+
 
     -webkit-font-smoothing: antialiased;
 
     -webkit-font-smoothing: antialiased;
 
     -webkit-overflow-scrolling: touch;
 
     -webkit-overflow-scrolling: touch;
Line 53: Line 399:
  
 
/* Titles */
 
/* Titles */
−
h1, h2, h3, h4, h5, h6,p {
+
h1, h2, h3, h4, h5, h6 {
−
font-family: 'Montserrat', sans-serif;
+
font-family: Comic Sans MS;
−
     font-weight: 600;
+
     font-weight: 700;
 
     color: #333;
 
     color: #333;
−
line-height:30pt;
 
 
}
 
}
  
Line 63: Line 408:
 
font-size: 35px;
 
font-size: 35px;
 
margin-top: 30px;
 
margin-top: 30px;
−
margin-bottom: 50px;
+
margin-bottom: 30px;
 
}
 
}
  
  
  
−
/* Paragraph  Typographic */
+
 
 
p {
 
p {
−
     line-height: 50px;
+
     line-height: 28px;
−
     margin-bottom: 35px;
+
     margin-bottom: 25px;
 
     font-size: 16px;
 
     font-size: 16px;
 +
font-family: Comic Sans MS;
 
}
 
}
  
Line 116: Line 462:
 
}
 
}
  
 +
.copyrights{
 +
text-indent:-9999px;
 +
height:0;
 +
line-height:0;
 +
font-size:0;
 +
overflow:hidden;
 +
}
  
 
.navbar {
 
.navbar {
Line 122: Line 475:
 
}
 
}
  
−
top1 {
 
−
padding-top: -20px;
 
−
]
 
 
.navbar-inverse {
 
.navbar-inverse {
−
padding-bottom: 70px;
+
padding-bottom: 30px;
−
padding-top: 70px;
+
padding-top: 0;
 
}
 
}
  
Line 137: Line 487:
 
.navbar-inverse .navbar-nav > li > a {
 
.navbar-inverse .navbar-nav > li > a {
 
color: white;
 
color: white;
 +
 
}
 
}
 +
  
 
.navbar-inverse .navbar-nav > li > a:hover {
 
.navbar-inverse .navbar-nav > li > a:hover {
Line 145: Line 497:
 
.navbar-brand {
 
.navbar-brand {
 
font-weight: 700;
 
font-weight: 700;
−
font-size: 20px;
+
font-size: 40px;
 
letter-spacing: 2px;
 
letter-spacing: 2px;
 +
backgroud: white;
 
}
 
}
  
 
.navbar-inverse .navbar-brand {
 
.navbar-inverse .navbar-brand {
 
color: white;
 
color: white;
 +
background-color: #FFA07A;
 +
 
}
 
}
  
 
.navbar-inverse .navbar-toggle {
 
.navbar-inverse .navbar-toggle {
 
border-color: transparent;
 
border-color: transparent;
 +
font-size: 12px;
 
}
 
}
  
Line 172: Line 528:
 
padding-top: 50px;
 
padding-top: 50px;
 
padding-bottom: 50px;
 
padding-bottom: 50px;
−
}
 
−
.lr {
 
−
        PADDING-LEFT: 100PX;
 
−
        padding-right: 100px;
 
 
}
 
}
 +
  
 
/* +++++ WRAP SECTIONS +++++ */
 
/* +++++ WRAP SECTIONS +++++ */
Line 195: Line 548:
 
#footer p {
 
#footer p {
 
color: white;
 
color: white;
 +
font-family: Comic Sans MS;
 
}
 
}
  
Line 280: Line 634:
 
}
 
}
 
   </style>
 
   </style>
−
 
 
    
 
    
−
  <!-- Bootstrap core CSS -->
 
−
    <link href="https://2017.igem.org/Template:BGIC-Union/bootstrap-css?action=raw&amp;ctype=text/css" rel="stylesheet">
 
−
<!-- animate -->
 
−
<link href="https://2017.igem.org/Template:BGIC-Union/animate-css?action=raw&amp;ctype=text/css" rel="stylesheet">
 
−
  <!-- carousel -->
 
−
  <link href="https://2017.igem.org/Template:BGIC-Union/owlcarousel-css?action=raw&amp;ctype=text/css" rel="stylesheet">
 
−
  <link href=" https://2017.igem.org/Template:BGIC-Union/fontawesomemin2-css?action=raw&amp;ctype=text/css" rel="stylesheet">
 
−
 
−
  <!-- main -->
 
−
    <link href="https://2017.igem.org/Team:BGIC-Union/finalcss/main-css?action=raw&amp;ctype=text/css" rel="stylesheet">
 
−
  <!-- slider -->
 
−
  <style type="text/css">
 
−
 
−
  /* Section Slider
 
−
========================================================= */
 
−
 
−
/* Carousel Slider
 
−
============================================== */
 
−
#slider_part {
 
−
    height: 900px;
 
−
    width: 100%;
 
−
    overflow: hidden;
 
−
background-color: #f2f2f2;
 
−
}
 
−
 
−
.carousel-caption {
 
−
    top: 30%;
 
−
    margin-top: -70px;
 
−
    text-shadow: none!important;
 
−
    left: 9%;
 
−
    right: 10%;
 
−
    -webkit-transition: all.3s ease 0s;
 
−
    -o-transition: all.3s ease 0s;
 
−
    transition: all.3s ease 0s;
 
−
    text-align: center;
 
−
}
 
−
 
−
.carousel-caption h3 {
 
−
    text-shadow: none;
 
−
    font-size: 70px;
 
−
    line-height: 92px;
 
−
    font-weight: 300;
 
−
    margin: 0 0px;
 
−
    transition-delay: .3s;
 
−
    -webkit-transition-delay: .3s;
 
−
    -o-transition-delay: .3s;
 
−
    text-transform: capitalize!important;
 
−
}
 
−
 
−
.carousel-caption p {
 
−
    font-size: 46px;
 
−
    line-height: 62px;
 
−
    font-weight: 500;
 
−
    color: #fff;
 
−
    transition-delay: .3s;
 
−
    -moz-transition-delay: .3s;
 
−
    -o-transition-delay: .3s;
 
−
    margin: 40px 0px;
 
−
    text-transform: uppercase;
 
−
font-family: Comic Sans MS;
 
−
}
 
−
 
−
.carousel-caption img {
 
−
    border: 2px solid#fff;
 
−
    padding: 10px 10px 10px 20px;
 
−
}
 
−
 
−
.line {
 
−
    width: 100px;
 
−
    height: 5px;
 
−
    color: #222;
 
−
    margin: 0px auto;
 
−
}
 
−
 
−
.carousel-caption p i {
 
−
    font-size: 7px;
 
−
    margin: 0px 3px;
 
−
    position: relative;
 
−
    top: -3px;
 
−
}
 
−
 
−
.carousel .item {
 
−
    -webkit-transition: opacity .3s;
 
−
    -moz-transition: opacity .3s;
 
−
    -ms-transition: opacity .3s;
 
−
    -o-transition: opacity .3s;
 
−
    transition: opacity .3s;
 
−
}
 
−
 
−
.btn-featured {
 
−
    text-decoration: none;
 
−
    position: relative;
 
−
    -webkit-transition: all .3s ease-in-out;
 
−
    -o-transition: all .3s ease-in-out;
 
−
    transition: all .3s ease-in-out;
 
−
    background: transparent;
 
−
    border-radius: 2px;
 
−
    z-index: 1;
 
−
    font-weight: 500;
 
−
    font-size: 18px;
 
−
    line-height: 20px;
 
−
    padding: 15px 40px !important;
 
−
    text-transform: uppercase;
 
−
    margin-top: 20px;
 
−
    margin-right: 10px;
 
−
}
 
−
 
−
.carousel-control.left,.carousel-control.right {
 
−
    background-image: none !important;
 
−
    background-repeat: no-repeat !important;
 
−
}
 
−
 
−
.carousel-control {
 
−
    width: 4% !important;
 
−
}
 
−
 
−
.carousel-caption img {
 
−
    height: auto;
 
−
}
 
−
 
−
.carousel-indicators {
 
−
    bottom: 100px;
 
−
    width: 10%;
 
−
    left: 45%;
 
−
    margin: 0 auto;
 
−
    bottom: 30%;
 
−
}
 
−
 
−
.carousel-indicators li {
 
−
    width: 20px !important;
 
−
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<li><a href="https://2017.igem.org/Team:BGIC-Union/Design" >design</a></li>
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<li><a href="https://2017.igem.org/Team:BGIC-Union/Demonstrate">demonstrate</a></li>
 
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<h4>BGIC-COLLEGE 2017</h4>
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Latest revision as of 03:27, 2 November 2017

Team：BGIC-Union




We are excited to have a chance to participate in the Fourth International Interlaboratory Study as a team in IGEM, 2017 and contribute our data as part of the GFP measurement. Interlab study requires us to transform eight plasmids into DH5α E.coli cell in order to measure OD 600 and Fluorescence of them as the time passes by.

Calibration:


1.Mea sure the Abs600 of both the LUDOX-S40 and water to obtain the correction factor.
2.Measure the fluorescence of a series of diluted fluorescein to draw the fluorescein standard curve.

Cell Measurement Procedure:



1. At first, we transform eight plasmids into DH5α E. Coli cell which include Positive control, Negative Control, D1-D6. After 12 hours, we pick one colony from each plate and inoculate each into a 50ml Falcon tube.

2. After a 12 hours incubation, we measure the primary OD600 and dilute bacteria solution into an OD600 of 0.02.

3. Finally, we measure the OD600 and Fluorescein every 2 hours and record the data into table.

Observations



1. It takes us much time to dilute the bacteria solution to the OD600 of 0.02. So the exact value of the concentration of bacteria solution may be incorrect.
2. As a team composed of high school students, sometimes we feel confused in understanding the procedures and the theory behind the instructions.
3. Since our data is very different compared to other teams’, maybe there are some improper operations or other mistakes in our study.


Finally, we sincerely appreciate BGI for permitting us to use the plate reader.