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Revision as of 03:36, 2 November 2017
Parts
Parts for the DNA System
| Registry Number | Code used in Lab | Brief Description | Key Details | Biobricks used | Other sequence sources |
|---|---|---|---|---|---|
| BBa_K2450101 | C100 | mCherry - TEV protease (non-cleaving) | Doesn’t self-cleave | BBa_J06504; BBa_K1319004 | Linker is Nd2 from https://www.hindawi.com/journals/bmri/2009/591923/tab2/ |
| BBa_K2450201 | C200 | Modified TetR with TEV cleavage site - CFP | TEV cleavage site in linker between DNA binding and dimerisation domains | BBa_K106669 | TEV cleavage site from Merck; CFP from AddGene |
| BBa_K2450251 | C250 | TetR - CFP | Normal TetR Class B | BBa_K106669 | CFP from AddGene |
| BBa_K2450301 | C300 | Tet promoter - RBS - YFP | DNA part, contains promoter and RBS to give signal of YFP | BBa_R0040; BBa_B0030 | eYFP from AddGene |
Parts for the Protein-Based System
| Registry Number | Code used in Lab | Brief Description | Key Details | Biobricks used | Other sequence sources |
|---|---|---|---|---|---|
| BBa_K2450401 | V100 | SpyTag - OmpA - sfGFP | SpyTag is between leader sequence and OmpA. Used sfGFP to help it fold out of cytoplasm | n/a | Alves et al, 2016 AddGene |
| BBa_K2450451 | v150 | OmpA - SpyTag - sfGFP | SpyTag is after OmpA - will be compared to BBa_K2450401. Used sfGFP to help it fold out of cytoplasm | n/a | Alves et al, 2016 |
| BBa_K2450501 | v200 | TorA Leader Sequence - SpyCatcher - sfGFP - Quencher | TEV cleavage site between sfGFP and the quenching peptide | BBa_K1319014 | Alves et al, 2016 |
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