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<p>We modeled LacILOV’s protein structure using a software called ITASSER. Our model approached the crystal structure of LacI and the LOV domain but also exposed undesirable interactions between the linker region and the functional domains. We then iteratively mutated the linker computationally and devised a set of substitutions that would improve the sensitivity of LacILOV to light.</p>
 
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Revision as of 20:27, 23 November 2017

Dry Lab

Ordinary Differential Equations

Capturing the dynamics of construct developed in the wet lab to further characterize its behaviour with Ordinary Differential Equations (ODE’s)

Analysis

Using Mathworks Simulink package we ran multiple simulations to obtain a clear picture of how our system behaves. Then with R we leverage the data from wetlab to derive key parameters.

Modelling

We modeled LacILOV’s protein structure using a software called ITASSER. Our model approached the crystal structure of LacI and the LOV domain but also exposed undesirable interactions between the linker region and the functional domains. We then iteratively mutated the linker computationally and devised a set of substitutions that would improve the sensitivity of LacILOV to light.

General Pages

Wet Lab

Dry Lab

Human Practices

iGEM Toronto