Difference between revisions of "Team:Munich/Testing"

Revision as of 22:42, 14 December 2017


Abstract
The ongoing crisis of increasing antibiotic resistance demands innovative preventive strategies. Recently, the RNA-targeting protein CRISPR-Cas13a has been used for highly sensitive DNA and RNA detection, promising diverse applications in point-of-care diagnostics. We integrated Cas13a in the detection unit of CascAID, our GMO-free diagnostic platform. CascAID combines an automated microfluidic device for rapid lysis and extraction of nucleic acids with a paper-based readout system. We demonstrated the performance of our device by targeting the 16S rRNA from E. coli. We improved the detection limit of our platform, using simulations to optimize our amplification scheme and the final readout. Conceived as a distributable platform for rapid point-of-care diagnostics, CascAID can be used to distinguish between bacterial and viral infections, thus minimizing the widespread use of antibiotics. Furthermore, Cas13a allows the fast design of target sequences, making our system adaptive to the emergence of new viral outbreaks or fast mutating pathogens.
Awards & Achievements

First Runner-up			Best Diagnostics Project
Best Model			Best Applied Design
Best Hardware			Best Software
Nominated for Best Wiki		Nominated for Best Presentation		Nominated for Best Poster
Nominated for Best Integrated Human Practices		Nominated for Best Best Education & Public Engagement		Nominated for Best Measurement
	Nominated for Best Composite Part		Nominated for Best Part Collection
Gallery

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