Difference between revisions of "Team:SJTU-BioX-Shanghai/Signal Pathway-cn"

Revision as of 00:14, 16 December 2017

信号通路

信号通路模型

我们的模型帮助描述了多因子可视化检测系统的细胞内反应。我们也有它来分析和评估我们的合成生物学系统。模型在Simbiology，MATLAB的工具箱下完成。

从图表中可以看出，我们找到了几个典型的变量变化的模式。实验结果与我们的模型吻合的很好。

Pathway Illustration

Figure1: Signal Pathway constructInducers enters the cell, activates receptor and binding with repressor protein, leading to antisense producing. Then antisense binds with STAR. Finally sfGFP expression starts.

Reaction

Simulation Results

Figure2: sfGFP degradation over time.

Figure3: antiSTAR1 variation over time.

Figure4: STAR & STAR_complex variation over time.

Figure5: sfGFP& STAR_complex variation over time.

From these figures, we find several typical patterns of species variation. And the Results of our experiments also fits these patterns well.

Parameters

Parameters	Reaction	Value	Reaction Type
maxVproduceAsRprotein	AsrR + RNA_polymerase -> AsrR + RNA_polymerase + AsrR_protein	0.6	MassAction
RNApolyConstant	All Reaction with Polymerase	1
kr_AsR_complex	As + AsrR_protein <-> AsR_complex	0.8	MassAction
kf_AsR_complex	As + AsrR_protein <-> AsR_complex	0.05	MassAction
maxVproduceanti	RNA_polymerase + Promoter_STAR1 -> RNA_polymerase + Promoter_STAR1 + antiSTAR1	60	Competitive-Inhibition
AsrRproteinRepressConstant	RNA_polymerase + Promoter_STAR1 -> RNA_polymerase + Promoter_STAR1 + antiSTAR1	10	Competitive-Inhibition
kf_STAR1complex	antiSTAR1 + STAR1 <-> STAR1_complex	50	MassAction
kr_STAR1complex	antiSTAR1 + STAR1 <-> STAR1_complex	0.8	MassAction
maxVproducesfGFP	Promoter_target + RNA_polymerase -> Promoter_target + RNA_polymerase + sfGFP	100	Competitive-Inhibition
antiRepressConstant	Promoter_target + RNA_polymerase -> Promoter_target + RNA_polymerase + sfGFP	10	Competitive-Inhibition
kd_AsrR	AsrR -> null	0.007	MassAction
kd_RNA_polymerase	RNA_polymerase -> null	0.007	MassAction
kd_AsrR_protein	AsrR_protein -> null	0.007	MassAction
kd_antiSTAR	antiSTAR1 -> null	0.007	MassAction
kd_STAR	STAR1 -> null	0.007	MassAction
kd_STARcomplex	STAR1_complex -> null	0.007	MassAction
kd_sfGFP	sfGFP -> null	0.007	MassAction

Table1

Species

Species	Description	Initial Value
AsrR	As repressor sequence	100
RNA_polymerase	RNA polymerase	2000
antiSTAR	antiSTAR sequence which binds with STAR to promote sfGFP	0
STAR	Small Transcription Activated RNA	200
STAR_complex	Complex of STAR&antiSTAR	100
sfGFP	Fluorescence Protein which displays green color	541.5
AsrR_protein	As repressor protein	200
As	As as an inducer	1000
AsR_complex	Complex of AsrR_protein&Promoter_STAR	0
Promoter_STAR	Promoter which generates antiSTAR sequence	60
Promoter_target	Promoter which is repressed by STAR	80

Table2

@@ Line 102: / Line 102: @@
                                  <li class="nav-item">
-                                     <a class="nav-link" href="#section1">Signal Pathway Model</a>
+                                     <a class="nav-link" href="#section1">信号通路模型</a>
                                  </li>
                                  <li class="nav-item">
-                                     <a class="nav-link" href="#section2">Pathway Illustration</a>
+                                     <a class="nav-link" href="#section2">通路图解</a>
                                  </li>
                                  <li class="nav-item">
-                                     <a class="nav-link" href="#section3">Reaction</a>
+                                     <a class="nav-link" href="#section3">反应</a>
                                  </li>
                                  <li class="nav-item">
-                                     <a class="nav-link" href="#section4">Simulation Results</a>
+                                     <a class="nav-link" href="#section4">模拟结果</a>
                                  </li>
                                  <li class="nav-item">
-                                     <a class="nav-link" href="#section5">Parameters</a>
+                                     <a class="nav-link" href="#section5">参数</a>
                                  </li>
                                  <li class="nav-item">
-                                     <a class="nav-link" href="#section6">Species</a>
+                                     <a class="nav-link" href="#section6">变量</a>
                                  </li>
                              </nav>
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                      <div class="col-12 col-lg-9">
-                         <div class="my-title h5-my-responsive" id="section1">Signal Pathway Model</div>
+                         <div class="my-title h5-my-responsive" id="section1">信号通路模型</div>
-                         <p>Our model helps describe intracellular interactions of our multifactorial visualized detection system. We also use it to analyze and evaluate our synthetic biology system. The model was built using Simbiology, a MATLAB toolbox.</p>
+                         <p>我们的模型帮助描述了多因子可视化检测系统的细胞内反应。我们也有它来分析和评估我们的合成生物学系统。模型在Simbiology，MATLAB的工具箱下完成。</p>
-                         <p>From these figures, we find several typical patterns of species variation. And the <a class="lin" target="_blank" href="https://2017.igem.org/Team:SJTU-BioX-Shanghai/Results">result</a> of our experiments also fits these patterns well.</p>
+                         <p>从图表中可以看出，我们找到了几个典型的变量变化的模式。<a class="lin" target="_blank" href="https://2017.igem.org/Team:SJTU-BioX-Shanghai/Results">实验结果</a>与我们的模型吻合的很好。 </p>
                          <div class="my-title h5-my-responsive" id="section2">Pathway Illustration</div>
                          <div class="figure-intro">