Difference between revisions of "Team:OUC-China"

Line 1: Line 1:
 
{{OUC-China}}
 
{{OUC-China}}
 
<html>
 
<html>
 +
<head>
 +
<meta charset="utf-8">
 +
<!-- TemplateBeginEditable name="doctitle" -->
 +
<title>无标题文档</title>
 +
<!-- TemplateEndEditable -->
 +
<!-- TemplateBeginEditable name="head" -->
 +
<!-- TemplateEndEditable -->
 +
<style type="text/css">
 +
        .igem_2017_menu_wrapper{display:none}
 +
.igem_2017_content_wrapper{width:100%;margin:0;float:none;font-size:17px;}
 +
        .igem_2017_content_wrapper h1{display:none;}
 +
.contentt{width: 100%;background: #0B486B;text-align: center;position: fixed;top: 0;margin: 0;padding: 0;}
 +
.contentt ul{display: inline-block;padding:0;list-style-type:none;}
 +
.contentt li{float: left;list-style-type:none;}
 +
.contentt a{display: block;text-decoration: none;color: white;width: 120px;line-height: 80px;background-color: #0B486B;font-size:18px;}
 +
.contentt a:hover{background-color:#266D96;color:white; }
 +
.top{position: fixed;bottom: 0;right: 0;}
 +
.top:hover{animation: upp 0.6s ;}
 +
@keyframes upp {
 +
0% {margin-bottom: 0;}
 +
50% {margin-bottom: 80px;}
 +
100% {margin-bottom: 0;}
 +
}
 +
#wrap{margin: 0 auto;width: 1200px;padding: 0;}
 +
.ab1{width: 100%;height: 450px;margin-bottom: 20px;margin-top: 80px;background-image:url(https://static.igem.org/mediawiki/2017/9/9a/T--OUC-China--ab1.jpeg);}
 +
.ab1 h2{padding-left: 420px;color:#79BD9A;font-size: 180%;padding-bottom:20px;}
 +
.ab1 p{width:800px;float: right;margin-bottom: 10px;font-size:20px;}
 +
.ab1 a{margin-left: 400px;text-decoration: none;color: #79BD9A;}
 +
.ab1 a img{vertical-align: middle;opacity: 0;}
 +
.ab1 a:hover img{opacity: 1;transition: 0.5s;}
 +
.ab1 .vidio {width: 800px;height: 250px;background-color: gray;color: white;text-align: center;line-height: 250px;float: right;margin-top: 10px;}
 +
.ab2{width: 100%;height: 450px;background-image:url(https://static.igem.org/mediawiki/2017/e/ef/T--OUC-China--ab2.jpeg);margin-bottom: 20px;}
 +
.ab2 h2{margin-left: 150px;padding-top: 30px;color:#79BD9A;font-size: 180%;}
 +
.ab2 p{width: 980px; margin-left: 150px;padding-top: 10px;font-size:18px;}
 +
.ab2 a{margin-left: 150px;text-decoration: none;color: #79BD9A;}
 +
.ab2 a img{vertical-align: middle;opacity: 0;}
 +
.ab2 a:hover img{opacity: 1;transition: 0.5s;}
 +
.ab3{width: 100%;height: 450px;background-image: url(https://static.igem.org/mediawiki/2017/7/76/T--OUC-China--ab3.jpeg);margin-bottom: 20px;}
 +
.ab3 h2{margin-left: 80px;padding-top: 30px;color:#79BD9A;font-size: 180%;}
 +
.ab3 p{width:1000px;padding-top: 20px;margin-left: 80px;padding-bottom: 20px;font-size:18px;}
 +
.ab3 a{margin-left: 220px;text-decoration: none;color: #79BD9A;}
 +
.ab3 a img{vertical-align: middle;opacity: 0;}
 +
.ab3 a:hover img{opacity: 1;transition: 0.5s;}
 +
.ab4{width: 100%;height: 450px;background-image: url(https://static.igem.org/mediawiki/2017/c/c4/T--OUC-China--ab4.jpeg);}
 +
.ab4 h2{margin-left: 245px;padding-top: 30px;color:#79BD9A;font-size: 180%;}
 +
.ab4 p{width: 700px;margin-left: 245px;padding-top: 20px;padding-bottom: 20px;font-size:20px;}
 +
.ab4 a{margin-left: 245px;text-decoration: none;color: #79BD9A;}
 +
.ab4 a img{vertical-align: middle;opacity: 0;}
 +
.ab4 a:hover img{opacity: 1;transition: 0.5s;}
 +
.ab5{width: 100%;height: 400px;background-image: url(https://static.igem.org/mediawiki/2017/c/c4/T--OUC-China--ab5.jpeg);}
 +
.ab5 h2{padding-top: 50px;color:#79BD9A;font-size: 180%;}
 +
.ab5 .wine{padding-top: 0;text-align: center;font-size: 150%;color:#266D96; }
 +
.ab5 p{width: 800px;padding-top: 20px;padding-bottom: 20px;font-size:20px;}
 +
.ab5 a{text-decoration: none;color: #79BD9A;}
 +
.ab5 a img{vertical-align: middle;opacity: 0;}
 +
.ab5 a:hover img{opacity: 1;transition: 0.5s;}
  
 +
#zanzhu{width:100%;background-color: #0B486B;color: white;text-align: center;padding: 0;}
 +
#zanzhu span{font-weight: bold;font-size: 150%;}
 +
        #zanzhu .zanzhushang{width:1200px;margin:0,auto;display:inline-block;}
 +
#zanzhu img{vertical-align: middle;margin:5px;}
 +
#zanzhu #qvbianyuan{margin:0;}
 +
   
 +
 +
   
 +
 +
</style>
 +
</head>
  
−
<div class="column full_size" >
+
<body>
−
<img src="http://placehold.it/2000x300/d3d3d3/f2f2f2">
+
<div class="contentt">  
 +
  <ul>
 +
<li><a href="#" style="height: 80px;width: 110px;"><img src="https://static.igem.org/mediawiki/2017/8/8e/T--OUC-China--guide.jpeg" width="110" height="80"/></a></li>
 +
<li><a href="#">Team</a></li>
 +
<li><a href="#" style="font-size: 17px;">Achievement</a></li>
 +
<li><a href="#">Project</a></li>
 +
<li><a href="#">Modeling</a></li>
 +
<li><a href="#">Result</a></li>
 +
<li><a href="#" style="font-size: 14px;">Human Practice</a></li>
 +
<li><a href="#">Parts</a></li>
 +
<li><a href="#">Notebook</a></li>
 +
<li><a href="#">Safety</a></li>
 +
  </ul>
 
</div>
 
</div>
−
 
+
−
<div class="column full_size" >
+
<a href="#top"><img class="top" src="https://static.igem.org/mediawiki/2017/b/b7/T--OUC-China--top.jpeg" width="150" height="116" /></a>
−
<h1> Welcome to iGEM 2017! </h1>
+
<div id="wrap">
−
<p>Your team has been approved and you are ready to start the iGEM season! </p>
+
<div class="ab1">
 +
     
 +
      <h2>We are OUC_China,we come from Qingdao!</h2>
 +
      <p>Qingdao is a beautiful city.However there are some annoying problems here.And that's what our team wants to solve this year.</p>
 +
      <a href="#"><img src="https://static.igem.org/mediawiki/2017/a/aa/T--OUC-China--jiantou.jpeg" width="30" height="30"/>&nbsp;More at our Human Practice page!</a>
 +
          <div class="vidio">此处是vidio</div>
 +
    </div>
 +
  <div class="ab2">
 +
        <h2>Step 1</h2>
 +
    <p>Now that we have plenty of Enteromorpha to deal with, we focus on the cellulose and hemicellulose which don’t have much added value in themselves thus wasn’t made good use of. And what we have done is fermentating them into ethyl alcohol.
 +
    Co-culture system is one biological system which has a great potential, especially in the aspect of its cooperative fermentation. Fiber corpuscle, one of the scaffold protein, is an existing efficient system in the nature. But in yeast, its expression may make the host metabolic burden too heavy. So we think of using E. coli to replace fibre corpuscle, which plays a similar effect to increase the efficiency of the enzyme.
 +
</p>
 +
            <a href="#"><img src="https://static.igem.org/mediawiki/2017/a/aa/T--OUC-China--jiantou.jpeg" width="30" height="30"/>&nbsp;More at our part1!</a>
 +
    </div>
 +
    <div class="ab3">
 +
        <h2>Step 2</h2>
 +
      <p>
 +
After pretreatment, we successfully transformed the cellulose and hemicellulose into cellobiose and xylose that are easier to use.
 +
The cellobiose is then transformed into ethyl alcohol through the intrinsic pathway of saccharomyces cerevisiae .
 +
As for the xylose,we transform it into xylulose and ultimately the ethyl alcohol.
 +
Thus we created two kinds of functional yeast which can live on special carbon source ---when we feed them with cellobiose and xylose, they will produce ethyl alcohol in return. What’s more, we can adjust the ratio between two yeasts to accomplish the best output corresponding to the original composition of Enteromorpha. Amazing!
 +
</p>
 +
    <a href="#"><img src="https://static.igem.org/mediawiki/2017/a/aa/T--OUC-China--jiantou.jpeg" width="30" height="30"/>&nbsp;More at our part2!</a>
 +
    </div>
 +
    <div class="ab4">
 +
        <h2>Step 3</h2>
 +
      <p>In addition to Enteromorpha contains numerous cellulose and hemicellulose, there are a large number of antioxidant polysaccharide substances. These polysaccharide substances can be used as materials for health care products. At the same time, we use the yeast to express resveratrol, an antioxidant substances either, to improve our antioxidant health care products.
 +
</p>
 +
    <a href="#"><img src="https://static.igem.org/mediawiki/2017/a/aa/T--OUC-China--jiantou.jpeg" width="30" height="30"/>&nbsp;More at our part3!</a>
 +
    </div>
 +
  <div class="ab5"> 
 +
    <h1 class="wine">At this point,we have created Enteromorpha health care wine!</h1>
 +
    <h2>Step extra</h2>
 +
    <p>When we compare the expression of eukaryotic and prokaryotic genes，eukaryotic gene expression vectors are larger because of single cistron，lengthy promoters and terminators.Therefore，we try to build a short and strong expression combination（mini-system）.It contains a small enough promoter and terminator.In addition, we tested to ensure optimal combination of promoter and terminator.
 +
</p>
 +
        <a href="#"><img src="https://static.igem.org/mediawiki/2017/a/aa/T--OUC-China--jiantou.jpeg" width="30" height="30"/>&nbsp;More at our part4!</a>
 +
    </div>
 
</div>
 
</div>
  
−
 
+
<div id="zanzhu">
−
<div class="clear"></div>
+
  <img id="qvbianyuan" src="https://static.igem.org/mediawiki/2017/6/6f/T--OUC-China--zanzhu.jpeg" width=100% />
−
 
+
  <p class="zanzhushang"><span>Thanks</span><br/>
−
<div class="column half_size" >
+
    <img src="https://static.igem.org/mediawiki/2017/e/eb/T--OUC-China--QNS.jpg"/>
−
<h5>Before you start: </h5>
+
    <img src="https://static.igem.org/mediawiki/2017/2/2a/T--OUC-China--ML.png" height="100"/>
−
<p> Please read the following pages:</p>
+
    <img src="https://static.igem.org/mediawiki/2017/5/51/T--OUC-China--NSG.png" height="100"/></p>
−
<ul>
+
  <p><span>Contact us:&nbsp;</span>oucigem@163.com<img src="https://static.igem.org/mediawiki/2017/b/b4/T--OUC-China--foot1.jpeg" width="50" height="50"/><img src="https://static.igem.org/mediawiki/2017/6/62/T--OUC-China--foot2.jpeg" width="50" height="50"></p>
−
<li>  <a href="https://2017.igem.org/Competition">Competition Hub</a> </li>
+
−
<li> <a href="https://2017.igem.org/Competition/Deliverables/Wiki">Wiki Requirements page</a></li>
+
−
<li> <a href="https://2017.igem.org/Resources/Template_Documentation">Template documentation</a></li>
+
−
</ul>
+
 
</div>
 
</div>
−
 
+
</body>
−
<div class="column half_size" >
+
−
<div class="highlight">
+
−
<h5> Styling your wiki </h5>
+
−
<p>You may style this page as you like or you can simply leave the style as it is. You can easily keep the styling and edit the content of these default wiki pages with your project information and completely fulfill the requirement to document your project.</p>
+
−
<p>While you may not win Best Wiki with this styling, your team is still eligible for all other awards. This default wiki meets the requirements, it improves navigability and ease of use for visitors, and you should not feel it is necessary to style beyond what has been provided.</p>
+
−
</div>
+
−
</div>
+
−
 
+
−
<div class="column full_size" >
+
−
<h5> Wiki template information </h5>
+
−
<p>We have created these wiki template pages to help you get started and to help you think about how your team will be evaluated. You can find a list of all the pages tied to awards here at the <a href="https://2017.igem.org/Judging/Pages_for_Awards">Pages for awards</a> link. You must edit these pages to be evaluated for medals and awards, but ultimately the design, layout, style and all other elements of your team wiki is up to you!</p>
+
−
 
+
−
</div>
+
−
 
+
−
 
+
−
 
+
−
 
+
−
<div class="column half_size" >
+
−
<h5> Editing your wiki </h5>
+
−
<p>On this page you can document your project, introduce your team members, document your progress and share your iGEM experience with the rest of the world! </p>
+
−
<p> <a href="https://2017.igem.org/wiki/index.php?title=Team:Example&action=edit"> </a>Use WikiTools - Edit in the black menu bar to edit this page</p>
+
−
 
+
−
</div>
+
−
 
+
−
 
+
−
<div class="column half_size" >
+
−
<h5>Tips</h5>
+
−
<p>This wiki will be your team’s first interaction with the rest of the world, so here are a few tips to help you get started: </p>
+
−
<ul>
+
−
<li>State your accomplishments! Tell people what you have achieved from the start. </li>
+
−
<li>Be clear about what you are doing and how you plan to do this.</li>
+
−
<li>You have a global audience! Consider the different backgrounds that your users come from.</li>
+
−
<li>Make sure information is easy to find; nothing should be more than 3 clicks away.  </li>
+
−
<li>Avoid using very small fonts and low contrast colors; information should be easy to read.  </li>
+
−
<li>Start documenting your project as early as possible; don’t leave anything to the last minute before the Wiki Freeze. For a complete list of deadlines visit the <a href="https://2017.igem.org/Calendar">iGEM 2017 calendar</a> </li>
+
−
<li>Have lots of fun! </li>
+
−
</ul>
+
−
</div>
+
−
 
+
−
 
+
−
<div class="column half_size" >
+
−
<h5>Inspiration</h5>
+
−
<p> You can also view other team wikis for inspiration! Here are some examples:</p>
+
−
<ul>
+
−
<li> <a href="https://2014.igem.org/Team:SDU-Denmark/"> 2014 SDU Denmark </a> </li>
+
−
<li> <a href="https://2014.igem.org/Team:Aalto-Helsinki">2014 Aalto-Helsinki</a> </li>
+
−
<li> <a href="https://2014.igem.org/Team:LMU-Munich">2014 LMU-Munich</a> </li>
+
−
<li> <a href="https://2014.igem.org/Team:Michigan"> 2014 Michigan</a></li>
+
−
<li> <a href="https://2014.igem.org/Team:ITESM-Guadalajara">2014 ITESM-Guadalajara </a></li>
+
−
<li> <a href="https://2014.igem.org/Team:SCU-China"> 2014 SCU-China </a></li>
+
−
</ul>
+
−
</div>
+
−
 
+
−
<div class="column half_size" >
+
−
<h5> Uploading pictures and files </h5>
+
−
<p> You can upload your pictures and files to the iGEM 2017 server. Remember to keep all your pictures and files within your team's namespace or at least include your team's name in the file name. <br />
+
−
When you upload, set the "Destination Filename" to <br><code>T--YourOfficialTeamName--NameOfFile.jpg</code>. (If you don't do this, someone else might upload a different file with the same "Destination Filename", and your file would be erased!)<br><br>
+
−
 
+
−
<a href="https://2017.igem.org/Special:Upload">
+
−
UPLOAD FILES
+
−
</a>
+
−
</p>
+
−
</div>
+
−
 
+
−
 
+
−
 
+
−
 
+
−
 
+
−
 
+
−
 
+
 
</html>
 
</html>

Revision as of 12:21, 28 July 2017

无标题文档

We are OUC_China,we come from Qingdao!

Qingdao is a beautiful city.However there are some annoying problems here.And that's what our team wants to solve this year.

 More at our Human Practice page!
此处是vidio

Step 1

Now that we have plenty of Enteromorpha to deal with, we focus on the cellulose and hemicellulose which don’t have much added value in themselves thus wasn’t made good use of. And what we have done is fermentating them into ethyl alcohol. Co-culture system is one biological system which has a great potential, especially in the aspect of its cooperative fermentation. Fiber corpuscle, one of the scaffold protein, is an existing efficient system in the nature. But in yeast, its expression may make the host metabolic burden too heavy. So we think of using E. coli to replace fibre corpuscle, which plays a similar effect to increase the efficiency of the enzyme.

 More at our part1!

Step 2

After pretreatment, we successfully transformed the cellulose and hemicellulose into cellobiose and xylose that are easier to use. The cellobiose is then transformed into ethyl alcohol through the intrinsic pathway of saccharomyces cerevisiae . As for the xylose,we transform it into xylulose and ultimately the ethyl alcohol. Thus we created two kinds of functional yeast which can live on special carbon source ---when we feed them with cellobiose and xylose, they will produce ethyl alcohol in return. What’s more, we can adjust the ratio between two yeasts to accomplish the best output corresponding to the original composition of Enteromorpha. Amazing!

 More at our part2!

Step 3

In addition to Enteromorpha contains numerous cellulose and hemicellulose, there are a large number of antioxidant polysaccharide substances. These polysaccharide substances can be used as materials for health care products. At the same time, we use the yeast to express resveratrol, an antioxidant substances either, to improve our antioxidant health care products.

 More at our part3!

At this point,we have created Enteromorpha health care wine!

Step extra

When we compare the expression of eukaryotic and prokaryotic genes，eukaryotic gene expression vectors are larger because of single cistron，lengthy promoters and terminators.Therefore，we try to build a short and strong expression combination（mini-system）.It contains a small enough promoter and terminator.In addition, we tested to ensure optimal combination of promoter and terminator.

 More at our part4!

Thanks

Contact us: oucigem@163.com