Difference between revisions of "Team:Hong Kong-CUHK/Results"

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   <h1>Charaterization</h1>
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   <p>Fluorescent protein is an important marker for qualitative analysis in validation test of toehold switch which detects the presence of trigger (viral) RNA.The presence of trigger RNA is reported by fold change of fluorescent protein expression compared with switch only by fluorescent assays and rely on the ability of fluorescent protein. More often, body fluid of different pH from vary organs (Figure 1) extracted as sample may disturb the expression of fluorescent protein&nbsp; due to pH-dependent protein folding and conformtaion. </p>
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   <p style="line-height: 1.2; margin-top: 0pt; margin-bottom: 0pt;"><span style="font-size: 14pt;"><strong><span style="color: #000000; font-family: Calibri; font-variant: normal; text-decoration: underline; vertical-align: baseline; background-color: transparent;">Overview</span></strong></span></p>
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<p style="line-height: 1.2; margin-top: 0pt; margin-bottom: 0pt;"><span style="color: #000000; font-family: Calibri; font-size: 14pt; font-variant: normal; font-weight: 400; text-decoration: none; vertical-align: baseline; background-color: transparent;">Fluorescent protein is an important marker for qualitative analysis in validation test of Toehold switch which detects the presence of trigger (viral) RNA.The presence of trigger RNA is reported by fold change of fluorescent protein expression compared with switch only by fluorescent assays and rely on the ability of fluorescent protein expression. More often, body fluid of different pH from vary organs extracted as sample may disturb the fluorescent level due to pH-dependent folding and conformation of proteins. </span></p>
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<p style="line-height: 1.2; margin-top: 0pt; margin-bottom: 0pt;"><span style="color: #000000; font-family: Calibri; font-size: 14pt; font-variant: normal; font-weight: 400; text-decoration: none; vertical-align: baseline; background-color: transparent;">To determine the effective range and optimize the protein expression level for clinical diagnosis, we have demonstrated their pH stability under different pH condition.</span></p>
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<p style="line-height: 1.2; margin-top: 0pt; margin-bottom: 0pt;"><span style="font-size: 14pt;"><strong><span style="color: #000000; font-family: Calibri; font-variant: normal; text-decoration: underline; vertical-align: baseline; background-color: transparent;"><br>Key Achievements</span></strong></span></p>
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<li style="list-style-type: disc; font-size: 12pt; font-family: Calibri; color: #000000; background-color: transparent; font-weight: 400; font-variant: normal; text-decoration: none; vertical-align: baseline;"><span style="color: #000000; font-family: Calibri; font-size: 14pt; font-variant: normal; font-weight: 400; text-decoration: none; vertical-align: baseline; background-color: transparent;">Characterised two fluorescent proteins for the registry to functionalize Toehold switch for clinical diagnosis. </span></li>
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<li style="list-style-type: disc; font-size: 12pt; font-family: Calibri; color: #000000; background-color: transparent; font-weight: 400; font-variant: normal; text-decoration: none; vertical-align: baseline;"><span style="color: #000000; font-family: Calibri; font-size: 14pt; font-variant: normal; font-weight: 400; text-decoration: none; vertical-align: baseline; background-color: transparent;">Proved the pH dependent stability of fluorescent proteins.</span></li>
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<p>Key achievement:</p>
 
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<li>Charaterized pH stability of fluorescnet protein in iGEM registry.</li>
 
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Revision as of 01:11, 3 August 2017





Results

Toehold Switch

This is overview about Toehold Switch.

Interlab

This is overview about Interlab.

Characterization

Overview

Fluorescent protein is an important marker for qualitative analysis in validation test of Toehold switch which detects the presence of trigger (viral) RNA.The presence of trigger RNA is reported by fold change of fluorescent protein expression compared with switch only by fluorescent assays and rely on the ability of fluorescent protein expression. More often, body fluid of different pH from vary organs extracted as sample may disturb the fluorescent level due to pH-dependent folding and conformation of proteins.

To determine the effective range and optimize the protein expression level for clinical diagnosis, we have demonstrated their pH stability under different pH condition.


Key Achievements

  • Characterised two fluorescent proteins for the registry to functionalize Toehold switch for clinical diagnosis.
  • Proved the pH dependent stability of fluorescent proteins.

Program

This is overview about Program.

Inspiration

See how other teams presented their results.