Difference between revisions of "Team:MSU-Michigan/Experiments"

Revision as of 20:09, 3 August 2017

Experiments

Describe the research, experiments, and protocols you used in your iGEM project. These should be detailed enough for another team to repeat your experiments.

Please remember to put all characterization and measurement data for your parts on the corresponding Registry part pages.

What should this page contain?

Protocols
Experiments
Documentation of the development of your project

Inspiration

Section 1

Some text..

Testing Fluorescence

End Point Fluorescence

Growth Fluorescence

Constructing Bioreactors

Purpose:

To create a large-scale biosensor that uses a single chamber to conduct current when inoculated with modified strains of Shewanella Oneidensis and induced by IPTG

Materials:

For each bioreactor:

250 mL mason jars
Rubber stoppers (2.5 cm tapering to 2 1/8 cm)
Titanium wire (~15cm per unit)
Carbon felt
Glass reference housing
Oxidized nickel wire + Small ~3 mm rubber stoppers
Large metal needles for sampling
3 mL plastic syringe (to be cut to act as housing for a counter-electrode)
Small magnetic stir bars
Needles and syringes of various size (sterile)

Chemicals:

Carbon paste suspended in Xylene
M5 Minimal Media (100 Mm Hepes)
KCl, crystal
dH2O
Bacto Agar
Vitamins/Minerals
200 mM Lactate
Spectinomycin Antibiotic
IPTG Inducer Stock

Bacterial strains:

Shewanella Oneidensis Δmtrb_GFP_mtrb (spec resistance)
Shewanella Oneidensis Δmtrb_GFP (spec resistance)
Shewanella Oneidensis Δmtrb

Other equipment:

Hot/stir plate
Multiple stir plate
Potentiostat
Autoclave

@@ Line 51: / Line 51: @@
 <body>
 <div class="w3-container">
-<button onclick="myFunction('openclose')" class="w3-btn w3-block w3-black w3-left-align">Open Section 1</button>
+<button onclick="myFunction('openclose')" class="w3-btn w3-block w3-black w3-left-align">sssss</button>
 <div id="openclose" class="w3-container w3-hide">
    <h4>Section 1</h4>