Difference between revisions of "Team:MSU-Michigan/Experiments"

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<h1>Experiments</h1>
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                <h1>Experiments</h1>
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<p>Describe the research, experiments, and protocols you used in your iGEM project. These should be detailed enough for another team to repeat your experiments.</p>
+
                <p>Describe the research, experiments, and protocols you used in your iGEM project. These should be detailed enough for another team to repeat your experiments.</p>
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<p>Please remember to put all characterization and measurement data for your parts on the corresponding Registry part pages.</p>
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                <p>Please remember to put all characterization and measurement data for your parts on the corresponding Registry part pages.</p>
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</div>
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            </div>
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<h5>What should this page contain?</h5>
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            <h5>What should this page contain?</h5>
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<ul>
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            <ul>
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<li>Protocols</li>
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                <li>Protocols</li>
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<li>Experiments</li>
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                <li>Experiments</li>
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<li>Documentation of the development of your project</li>
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                <li>Documentation of the development of your project</li>
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</ul>
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            </ul>
  
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<div class="column half_size">
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            <div class="column half_size">
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<h5>Inspiration</h5>
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                <h5>Inspiration</h5>
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<ul>
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                <ul>
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<li><a href="https://2014.igem.org/Team:Colombia/Protocols">2014 Colombia </a></li>
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                    <li><a href="https://2014.igem.org/Team:Colombia/Protocols">2014 Colombia </a></li>
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<li><a href="https://2014.igem.org/Team:Imperial/Protocols">2014 Imperial </a></li>
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                    <li><a href="https://2014.igem.org/Team:Imperial/Protocols">2014 Imperial </a></li>
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<li><a href="https://2014.igem.org/Team:Caltech/Project/Experiments">2014 Caltech </a></li>
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                    <li><a href="https://2014.igem.org/Team:Caltech/Project/Experiments">2014 Caltech </a></li>
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</ul>
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                </ul>
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            <body>
  
  
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<div class="w3-container">
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                <div class="w3-container">
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<h2>Testing the Strains</h2>
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                    <h2>Testing the Strains</h2>
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<button onclick="myFunction('openclose')" class="w3-btn w3-block w3-black w3-left-align">End Point Fluorescence</button>
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                    <button onclick="myFunction('openclose')" class="w3-btn w3-block w3-black w3-left-align">End Point Fluorescence</button>
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<div id="openclose" class="w3-container w3-hide">
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                    <div id="openclose" class="w3-container w3-hide">
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  <h4>Title</h4>
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                        <h4>Title</h4>
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  <p>This procedure is to test the fluorescence of modified <em>Shewanella oneidensis MR-1</em>.</p>
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                        <p>This procedure is to test the fluorescence of modified <em>Shewanella oneidensis MR-1</em>.</p>
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</div>
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                    </div>
  
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<button onclick="myFunction('openclose2')" class="w3-btn w3-block w3-black w3-left-align">Growth Fluorescence</button>
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                    <button onclick="myFunction('openclose2')" class="w3-btn w3-block w3-black w3-left-align">Growth Fluorescence</button>
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<div id="openclose2" class="w3-container w3-hide">
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                    <div id="openclose2" class="w3-container w3-hide">
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  <h4>Title</h4>
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                        <h4>Title</h4>
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  <p>This procedure is to test the fluorescence of modified <em>Shewanella oneidensis MR-1</em>.</p>
+
                        <p>This procedure is to test the fluorescence of modified <em>Shewanella oneidensis MR-1</em>.</p>
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</div>
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                    </div>
  
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<button onclick="myFunction('openclose3')" class="w3-btn w3-block w3-black w3-left-align">Bioreactors Current Measurement</button>
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                    <button onclick="myFunction('openclose3')" class="w3-btn w3-block w3-black w3-left-align">Bioreactors Current Measurement</button>
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<div id="openclose3" class="w3-container w3-hide">
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                    <div id="openclose3" class="w3-container w3-hide">
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  <h4>Title</h4>
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                        <h4>Title</h4>
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  <p>This procedure is to test the current output of modified <em>Shewanella oneidensis MR-1</em>.</p>
+
                        <p>This procedure is to test the current output of modified <em>Shewanella oneidensis MR-1</em>.</p>
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</div>
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                    </div>
  
  
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<h2>Building Measurement Devices</h2>
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                    <h2>Building Measurement Devices</h2>
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<button onclick="myFunction('openclose4')" class="w3-btn w3-block w3-black w3-left-align">Building Bioreactors</button>
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                    <button onclick="myFunction('openclose4')" class="w3-btn w3-block w3-black w3-left-align">Building Bioreactors</button>
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<div id="openclose4" class="w3-container w3-hide">
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                    <div id="openclose4" class="w3-container w3-hide">
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<h5>Purpose: </h5>
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                        <h5>Purpose: </h5>
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To create a large-scale liquid biosensor that uses a single chamber to conduct current when inoculated with modified strains of <em>Shewanella Oneidensis</em> and induced by IPTG
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                        To create a large-scale liquid biosensor that uses a single chamber to conduct current when inoculated with modified strains of <em>Shewanella Oneidensis</em> and induced by IPTG
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<h5>Materials:</h5>
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                        <h5>Materials:</h5>
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<h6> For each bioreactor:</h6>
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                        <h6> For each bioreactor:</h6>
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<ul>
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                        <ul>
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<li>250 mL mason jars </li>
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                            <li>250 mL mason jars </li>
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<li>Rubber stoppers (2.5 cm tapering to 2 1/8 cm)</li>
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                            <li>Rubber stoppers (2.5 cm tapering to 2 1/8 cm)</li>
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<li>Titanium wire (~15cm per unit)</li>
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                            <li>Titanium wire (~15cm per unit)</li>
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<li>Carbon felt</li>
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                            <li>Carbon felt</li>
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<li>Glass reference housing</li>
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                            <li>Glass reference housing</li>
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<li>Oxidized nickel wire + Small ~3 mm rubber stoppers</li>
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                            <li>Oxidized nickel wire + Small ~3 mm rubber stoppers</li>
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<li>Large metal needles for sampling  </li>
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                            <li>Large metal needles for sampling  </li>
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<li>3 mL plastic syringe (to be cut to act as housing for a counter-electrode)</li>
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                            <li>3 mL plastic syringe (to be cut to act as housing for a counter-electrode)</li>
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<li>Small magnetic stir bars </li>
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                            <li>Small magnetic stir bars </li>
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<li>Needles and syringes of various size (sterile) </li>
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                            <li>Needles and syringes of various size (sterile) </li>
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</ul>
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                        </ul>
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<h6>Chemicals:</h6>
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                        <h6>Chemicals:</h6>
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<ul>
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                        <ul>
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<li>Carbon paste suspended in Xylene </li>
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                            <li>Carbon paste suspended in Xylene </li>
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<li>M5 Minimal Media (100 Mm Hepes) </li>
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                            <li>M5 Minimal Media (100 Mm Hepes) </li>
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<li>KCl, crystal</li>
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                            <li>KCl, crystal</li>
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<li>dH2O </li>
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                            <li>dH2O </li>
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<li>Bacto Agar </li>
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                            <li>Bacto Agar </li>
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<li>Vitamins/Minerals</li>
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                            <li>Vitamins/Minerals</li>
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<li>200 mM Lactate</li>
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                            <li>200 mM Lactate</li>
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<li>Spectinomycin Antibiotic </li>
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                            <li>Spectinomycin Antibiotic </li>
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<li>IPTG Inducer Stock</li>
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                            <li>IPTG Inducer Stock</li>
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</ul>
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                        </ul>
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Bacterial strains:  
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                        Bacterial strains:  
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<ul>
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                        <ul>
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<li>Shewanella Oneidensis Δmtrb_GFP_mtrb (spec resistance) </li>
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                            <li>Shewanella Oneidensis Δmtrb_GFP_mtrb (spec resistance) </li>
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<li>Shewanella Oneidensis Δmtrb_GFP (spec resistance) </li>
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                            <li>Shewanella Oneidensis Δmtrb_GFP (spec resistance) </li>
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<li>Shewanella Oneidensis Δmtrb </li>
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                            <li>Shewanella Oneidensis Δmtrb </li>
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</ul>
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                        </ul>
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Other equipment:  
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                        Other equipment:  
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<ul>
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                        <ul>
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<li>Hot/stir plate</li>
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                            <li>Hot/stir plate</li>
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<li>Multiple stir plate </li>
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                            <li>Multiple stir plate </li>
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<li>Potentiostat</li>  
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                            <li>Potentiostat</li>  
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<li>Autoclave</li>
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                            <li>Autoclave</li>
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</ul>
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                        </ul>
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</div>
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                    </div>
  
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</div>
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                    <button onclick="myFunction('openclose5')" class="w3-btn w3-block w3-black w3-left-align">Paper Microbial Fuel Cells</button>
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                    <div id="openclose5" class="w3-container w3-hide">
 +
                        <h4>Creating Paper Microbial Fuel Cells</h4>
 +
                        <p>This procedure is to test the current output of modified <em>Shewanella oneidensis MR-1</em> in a paper cell.</p>
 +
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Revision as of 20:35, 3 August 2017

Experiments

Describe the research, experiments, and protocols you used in your iGEM project. These should be detailed enough for another team to repeat your experiments.

Please remember to put all characterization and measurement data for your parts on the corresponding Registry part pages.

What should this page contain?
  • Protocols
  • Experiments
  • Documentation of the development of your project
Inspiration

Testing the Strains

Title

This procedure is to test the fluorescence of modified Shewanella oneidensis MR-1.

Title

This procedure is to test the fluorescence of modified Shewanella oneidensis MR-1.

Title

This procedure is to test the current output of modified Shewanella oneidensis MR-1.

Building Measurement Devices

Purpose:
To create a large-scale liquid biosensor that uses a single chamber to conduct current when inoculated with modified strains of Shewanella Oneidensis and induced by IPTG
Materials:
For each bioreactor:
  • 250 mL mason jars
  • Rubber stoppers (2.5 cm tapering to 2 1/8 cm)
  • Titanium wire (~15cm per unit)
  • Carbon felt
  • Glass reference housing
  • Oxidized nickel wire + Small ~3 mm rubber stoppers
  • Large metal needles for sampling
  • 3 mL plastic syringe (to be cut to act as housing for a counter-electrode)
  • Small magnetic stir bars
  • Needles and syringes of various size (sterile)
Chemicals:
  • Carbon paste suspended in Xylene
  • M5 Minimal Media (100 Mm Hepes)
  • KCl, crystal
  • dH2O
  • Bacto Agar
  • Vitamins/Minerals
  • 200 mM Lactate
  • Spectinomycin Antibiotic
  • IPTG Inducer Stock
Bacterial strains:
  • Shewanella Oneidensis Δmtrb_GFP_mtrb (spec resistance)
  • Shewanella Oneidensis Δmtrb_GFP (spec resistance)
  • Shewanella Oneidensis Δmtrb
Other equipment:
  • Hot/stir plate
  • Multiple stir plate
  • Potentiostat
  • Autoclave

Creating Paper Microbial Fuel Cells

This procedure is to test the current output of modified Shewanella oneidensis MR-1 in a paper cell.