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April 2016 | April 2016 | ||
− | < | + | <ul> |
− | + | <li style="margin-left: 24.0pt; text-indent: -24.0pt;">Settlement of lab duties and position.</li> | |
− | < | + | <li style="margin-left: 24.0pt; text-indent: -24.0pt;">Lab safety Training.</li> |
− | + | <li style="margin-left: 24.0pt; text-indent: -24.0pt;">Started regular meetings every week.</li> | |
− | + | </ul> | |
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May 2016 | May 2016 | ||
− | + | <ul> | |
− | + | <li style="margin-left: 24.0pt; text-indent: -24.0pt;">Recruitment of ideas and prepare preliminary data for selection.</li> | |
− | <ul>Recruitment of ideas and prepare preliminary data for selection. Project related to pancreatic cancer diagnosis by Toehold Switch is selected.</ul> | + | <li style="margin-left: 24.0pt; text-indent: -24.0pt;">Project related to pancreatic cancer diagnosis by Toehold Switch is selected</li> |
− | + | </ul> | |
− | </ | + | <p>August 2016</p> |
− | + | <ul> | |
− | < | + | <li style="margin-left: 24.0pt; text-indent: -24.0pt;">Cloning of switches and itscorresponding triggers into pSB1A2 and pSB1 with significant “ON/OFF”ratio in vivo.</li> |
− | + | </ul> | |
− | + | <p> </p> | |
− | + | <p>September 2016</p> | |
− | + | <ul> | |
− | + | <li style="margin-left: 24.0pt; text-indent: -24.0pt;">Identified problems to design Toehold Switch manually and outline programme functions for semi-automatic toehold switch design.</li> | |
− | + | <li style="margin-left: 24.0pt; text-indent: -24.0pt;">Modified the switch by using new chromoprotein (amajLime and amilCP) by traditional cloning method to produce intuitive readout.</li> | |
− | + | </ul> | |
− | + | <p> </p> | |
− | + | <p>October 2016</p> | |
− | + | <ul> | |
− | + | <li style="margin-left: 24.0pt; text-indent: -24.0pt;">Reviewing the impact and necessity of technology in nowadays society, we decided to use Toehold Switch to subtype Influenza A.</li> | |
− | + | </ul> | |
− | + | <p>November 2016</p> | |
− | + | <ul> | |
− | + | <li style="margin-left: 24.0pt; text-indent: -24.0pt;">Brainstorming and literature reviewing on how to maximize the“ON/OFF” ratio of Toehold switch.</li> | |
− | + | <li style="margin-left: 24.0pt; text-indent: -24.0pt;">Constructed programs which embedded with Influenza A sequence database and Vienna RNA package.</li> | |
+ | </ul> | ||
+ | <p>December 2016</p> | ||
+ | <ul> | ||
+ | <li style="margin-left: 24.0pt; text-indent: -24.0pt;">Failed to test the system in cell free system due to insufficient materials and resources.</li> | ||
+ | <li style="margin-left: 24.0pt; text-indent: -24.0pt;">T7-rna-polymerase amplification system is cloning to yield higher “ON/OFF”</li> | ||
+ | <li style="margin-left: 24.0pt; text-indent: -24.0pt;">Requested for sponsorship.</li> | ||
+ | <li style="margin-left: 24.0pt; text-indent: -24.0pt;">Skyped the Pakistan iGEM team to explore the potential of collaboration.</li> | ||
+ | <li style="margin-left: 24.0pt; text-indent: -24.0pt;">Continued fundraising.</li> | ||
+ | </ul> | ||
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Revision as of 13:56, 10 August 2017