Difference between revisions of "Team:SiCAU-China"
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| − | <h1>A Positive Feedback System to Detection</h1> | + | <h1 align="center" class="STYLE1"><img src="https://static.igem.org/mediawiki/2017/5/5c/T-SICAU-eye.jpg" width="100%" height="100%"></h1> |
| − | <p | + | <h1 align="center" class="STYLE6">A Positive Feedback System to Detection</h1> |
| − | Antibiotic is one of the most popular drug in the world, it can be used in medical treatment, agriculture, food processing, research and so on. But overusing antibiotics may bring antibiotics residue to environment, which will cause potential damage to human and impact the ecotope. Nowadays there are some physical and chemical methods to detect antibiotics residue, but they often need high cost and complicated process. So this year our team focus on Biosensor, said to be a easier way to detect than traditional methods at a lower price, to realize the trace detection.< | + | <p>Antibiotic is one of the most popular drug in the world, it can be used in medical treatment, agriculture, food processing, research and so on. But overusing antibiotics may bring antibiotics residue to environment, which will cause potential damage to human and impact the ecotope. Nowadays there are some physical and chemical methods to detect antibiotics residue, but they often need high cost and complicated process. So this year our team focus on Biosensor, said to be a easier way to detect than traditional methods at a lower price, to realize the trace detection. </p> |
| − | The positive feedback loop has been widely applied in synthetic biology, such as logic gates, oscillators, bistable circuit. Besides, the positive feedback loop is also an ultra-sensitive circuit, just like Polymerase Chain Reaction (PCR), even be used in quantitative expression detected. So we engineered a biosensor, which will be established a positive feedback loop using luxI/luxR system in E.coli, to achieve trace detection. Similar to the detect theory of real-time quantitative PCR, there will be a relation between the content of input signal and the time to open the loop. We will use this system to do some antibiotics leftover detections. | + | <p>The positive feedback loop has been widely applied in synthetic biology, such as logic gates, oscillators, bistable circuit. Besides, the positive feedback loop is also an ultra-sensitive circuit, just like Polymerase Chain Reaction (PCR), even be used in quantitative expression detected. So we engineered a biosensor, which will be established a positive feedback loop using luxI/luxR system in E.coli, to achieve trace detection. Similar to the detect theory of real-time quantitative PCR, there will be a relation between the content of input signal and the time to open the loop. We will use this system to do some antibiotics leftover detections.</p> |
| − | </p> | + | <p class="STYLE4"> <span class="STYLE5">more coming soon</span> ......</p> |
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Revision as of 09:50, 14 August 2017
A Positive Feedback System to Detection
Antibiotic is one of the most popular drug in the world, it can be used in medical treatment, agriculture, food processing, research and so on. But overusing antibiotics may bring antibiotics residue to environment, which will cause potential damage to human and impact the ecotope. Nowadays there are some physical and chemical methods to detect antibiotics residue, but they often need high cost and complicated process. So this year our team focus on Biosensor, said to be a easier way to detect than traditional methods at a lower price, to realize the trace detection.
The positive feedback loop has been widely applied in synthetic biology, such as logic gates, oscillators, bistable circuit. Besides, the positive feedback loop is also an ultra-sensitive circuit, just like Polymerase Chain Reaction (PCR), even be used in quantitative expression detected. So we engineered a biosensor, which will be established a positive feedback loop using luxI/luxR system in E.coli, to achieve trace detection. Similar to the detect theory of real-time quantitative PCR, there will be a relation between the content of input signal and the time to open the loop. We will use this system to do some antibiotics leftover detections.
more coming soon ......
