Difference between revisions of "Team:WHU-China/InterLab"

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<h3>★  ALERT! </h3>
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<p>This page is used by the judges to evaluate your team for the <a href="https://2017.igem.org/Judging/Medals">medal criterion</a> or <a href="https://2017.igem.org/Judging/Awards"> award listed above</a>. </p>
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<p> Delete this box in order to be evaluated for this medal criterion and/or award. See more information at <a href="https://2017.igem.org/Judging/Pages_for_Awards"> Instructions for Pages for awards</a>.</p>
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          <ul class="nv">
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            <li id="nv0" style="width:56px"><a href="#">logo</a></li>
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            <li id="nv1">
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              <a>Project</a>
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              <ul>
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                <li id="nv11"><a href="#">Description</a></li>
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                <li id="nv12"><a href="#">Design</a></li>
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                <li id="nv13"><a href="#">Experiments</a></li>
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                <li id="nv14"><a href="#">Results</a></li>
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              </ul>
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              <a>Parts</a>
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                <li id="nv21"><a href="#">Basic Part</a></li>
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                <li id="nv22"><a href="#">Composite Part</a></li>
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              </ul>
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            </li>
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            <li id="nv3"><a href="#">Interlab</a></li>
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            <li id="nv4"><a href="#">Models</a></li>
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              <a>Human Practices</a>
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                <li id="nv51"><a href="#">Activities</a></li>
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                <li id="nv52"><a href="#">Silver</a></li>
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                <li id="nv53"><a href="#">Gold</a></li>
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              </ul>
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            <li id="nv6"><a href="#">Safety</a></li>
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              <a>Team</a>
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              <ul>
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                <li id="nv71"><a href="#">Team Members</a></li>
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                <li id="nv72"><a href="#">Collaborations</a></li>
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              </ul>
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<h6>Description</h6>
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<ul id="cont">
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  <li><a href="#Brief">1 Brief introduction in RdhANP</a></li>
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  <li><a href="#Description">2 Description of our operating bacteria</a></li>
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  <li><a href="#Modularization">3 Modularization: Overview</a></li>
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  <li><a href="#protocol">4 Our protocol</a></li>
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  <li><a href="#Appendix">5 Appendix</a></li>
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<h1>InterLab</h1>
 
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<h3>Bronze Medal Criterion #4</h3>
 
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<p><b>Standard Tracks:</b> Participate in the Interlab Measurement Study and/or improve the characterization of an existing BioBrick Part or Device and enter this information on that part's Main Page in the Registry. The part that you are characterizing must NOT be from a 2017 part number range.
 
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For teams participating in the <a href="https://2017.igem.org/Competition/InterLab_Study">InterLab study</a>, all work must be shown on this page.
 
  
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          <h1 id="Brief">Brief introduction in RdhANP</h1>
  
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          <p>&nbsp;&nbsp;&nbsp;&nbsp;RdhANP is a native protein of Nitratireducror pacificus pht-3B. It is codon-optimized for operated and expression in Bacillus magaterium. RdhANP can catalyze the reduction of ortho-halogenated phenolic compound, which endows it with biological dehalogenation function. Different from other reductive proteins that are membrane-associated and oxygen-sensitive in their family, RdhANP is a soluble cytoplasmic one and oxygen-tolerant, which is fairly good for heterologous expression in other chassis. However, this enzyme displays a strict requirement for o-halogenated phenolic substrates and B12 cofactor; it also should under reductive condition using either reduced methyl viologen or NADPH. </p>
  
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Revision as of 12:45, 24 August 2017

Brief introduction in RdhANP

    RdhANP is a native protein of Nitratireducror pacificus pht-3B. It is codon-optimized for operated and expression in Bacillus magaterium. RdhANP can catalyze the reduction of ortho-halogenated phenolic compound, which endows it with biological dehalogenation function. Different from other reductive proteins that are membrane-associated and oxygen-sensitive in their family, RdhANP is a soluble cytoplasmic one and oxygen-tolerant, which is fairly good for heterologous expression in other chassis. However, this enzyme displays a strict requirement for o-halogenated phenolic substrates and B12 cofactor; it also should under reductive condition using either reduced methyl viologen or NADPH.