Team:Aix-Marseille/Bacteriophages
Engineering bacteriophages
M13 phages
M13 is a filamentous phage that infects E. coli that carry the F-episome. Active infection with M13 does not kill the host cell.
Phages-like particules design
Bacteriophages are capable of expressing their genomes, and generating new copies of themselves. We choose to limit the phage ability to reproduce itself in order to contain it. As it is possible to produce recombinant viruses that express foreign proteins, it is possible to restrain their capacity to reproduce themself. [1]
Virus-like particles (VLPs) are multiprotein structures that mimic the organization and conformation of authentic native viruses but lack the viral genome. They have been applied not only as prophylactic and therapeutic vaccines but also as vehicles in drug and gene delivery and, more recently, as tools in nanobiotechnology. [1]
In this project, we want to creat a M13-like phage like particle, in order to inject toxin in the bacterium.
References
