Team:Heidelberg/internal tools
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From 2014.igem.org
Internal Tools
Number of mutations and mutated sequences
Expected number of mutations in a single sequence: $$\frac{N_{mutations}}{L_{Sequence}} = N_{generations} * r_{mutation} = t_{total} * \frac{1}{\Phi} * r_{mutation}$$
The expected share of sequences that shows at least one mutation in \(L_{Sequence}\) bp is the probability that \(L_{sequence}\) basepairs stay unchanged when \(\frac{N_{mutations}}{L_{Sequence}}\) mutations are expected: $$\frac{N_{mutated}}{N_{Sequences}} = p(N_{mutations}=0) = (1-\frac{N_{mutations}}{L_{Sequence}}) * L_{Sequence} = L_{Sequence} - N_{mutations} $$
Set \(\Phi\) to zero to use the number of generations for the calculation.
Consider \(L_{Sequence}\) as the number of basepairs that is expected to be mutated. If half of the sequence you are interested in, is highly conserved choose a lower \(L_{Sequence}\).
\(\frac{N_{mutations}}{L_{Sequence}} =\) %(bp/bp).
\(N_{mutations} =\) bp.
The share of sequences that shows at least one mutation in \(L_{Sequence}\) bp, \(\frac{N_{mutated}}{N_{Sequences}} = \) % of sequences
