Team:Bristol/Experiments

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PCR for Nap and Nrf fragments

We split the Nap and Nrf operons into 4 fragments for each and had these synthesised. We then used PCR to amplify each fragment, so that Gibson assembly could then be used to assemble the fragments into whole operons. A gel showing the purified PCR products is shown below:


Gel showing the purified PCR products


NB: Nap1a contains a 3' overlap, to allow subsequent joining to the Nrf4 fragment using Gibson assembly.
Nap1b instead contains the BioBrick prefix, and has the double terminator (found before the Nap genes) removed. Assembly using this fragment will allow Nap to be submitted as an individual part without the double terminator, which is unnecessary when Nrf is not present.
Please see our Parts page for more information.