Team:Kyoto/Experiments

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Material&Methods

  1. Parts
  2. Primer list
  3. Materials
  4. Methods
  5. Parts

    Primer list

    Primer name Sequence Length Tm GC% Designer Manufacturer

    Materials

    Kit

    Name Supplier

    Restriction Enzyme

    Name Supplier

    Name Supplier

    DNA Ligase

    Name Supplier

    Marker

    Name Supplier

    Name Supplier

    Antibiotics

    Name Supplier

    Equipment

    Name Supplier

    Backbones

    Name Supplier

    Buffer

    Name Supplier

    SDSPAGE/Western blotting

    Name Supplier

    Methods

    Miniprep

    Gel Extraction

    Restriction Enzyme Digestion

    Ligation

    Transformation

    PCR

    Sequenicing

    RT-PCR

    Basic protcol of Western blotting

    Western blotting(Membrane fraction)