var Json = {
"March":{
"D27":"1.The production of competent cells by Kit",
"D28":"1.SDS-PAGE
2.T-vector transformation",
"D29":"1.GDNA was extracted by CTAB
2.T1 plasmid was inoculated
3.Stock"
},
"April":{
"D5":"1.Plasmid DNA extraction
2.T2 generation plasmid inoculation",
"D6":"1.Pave board
2.Inoculate to LB liquid medium",
"D7":"1.extract gDNA",
"D10":"1.GDNA was extracted by CTAB",
"D11":"1.Extraction of yeast gDNA by CTAB",
"D12":"1.Agarose gel electrophoresis",
"D13":"1.Cloning experiments of Control DNA fragments",
"D17":"1.Extraction of yeast gDNA by CTAB",
"D18":"1.Agarose gel electrophoresis",
"D19":"1.Extraction plasmid
2.Agarose gel electrophoresis
3.AMP conversion plate validation",
"D20":"1.Cloning experiments of Control DNA fragments
2.Extraction of yeast gDNA by CTAB",
"D21":"1.Agarose gel electrophoresis",
"D24":"1.Cloning experiments of Control DNA fragments
2.Extraction plasmid
3.Agarose gel electrophoresis
4.PCR",
"D25":"1.Purification of GDNA
2.Inoculated taste bacteria fluid
3.Agarose gel electrophoresis
4.PCR",
"D26":"1.inoculation
2.Transformation of preparing competent cells and competent
3.Agarose gel electrophoresis",
"D27":"1.Transformed plate observation
2.Enzyme digestion was performed with a kit
3.Agarose gel electrophoresis
4.PCR",
"D28":"1.Extraction plasmid
2.Agarose gel electrophoresis"
},
"May":{
"D1":"1.Transformed light off plasmid
2.Stock",
"D2":"1.Light off transformed plates were observed
2.Stock",
"D3":"1.To configure Streptomycin
2.Plasmid connection
3.Plasmid purification",
"D4":"1.Extraction plasmid
2.Inoculate to LB liquid medium",
"D5":"1.Streptomycin gradient plate observation
2.Lightoff plasmid was extracted
3.Agarose gel electrophoresis",
"D7":"1.Streptomycin test
2.Do light on system validation",
"D9":"1.Extraction plasmid
2.Agarose gel electrophoresis
3.Stock
4.inoculation
5.light on T1 generation transformation",
"D10":"1.Extraction plasmid
2.Agarose gel electrophoresis
3.Rubber cutting
4.Agarose gel electrophoresis",
"D11":"1.Agarose gel electrophoresis plastic recycling
2.Agarose gel electrophoresis",
"D15":"1.Stock
2.Agarose gel electrophoresis plastic recycling
3.Agarose gel electrophoresis",
"D16":"1.Agarose gel electrophoresis plastic recycling
2.Agarose gel electrophoresis
3.To configure Streptomycin
4.light on T1 generation transformation",
"D17":"1.light on T1 generation transformation
2.Stock"
},
"June":{
"D26":"1.Learning HPLC operations
2.Enzyme digestion",
"D27":"1.Rubber cutting
2.Agarose gel electrophoresis",
"D28":"1.Agarose gel electrophoresis plastic recycling
2.Agarose gel electrophoresis",
"D29":"1.Enzyme digestion
2.Agarose gel electrophoresis",
"D30":"1.Agarose gel electrophoresis plastic recycling
2.Agarose gel electrophoresis"
},
"July":{
"D3":"1.Enzyme digestion
2.inoculation",
"D4":"1.Extraction plasmid
2.Agarose gel electrophoresis",
"D5":"1.Purified plasmid
2.Agarose gel electrophoresis
3.Enzyme digestion",
"D6":"1.Agarose gel electrophoresis
2.Agarose gel electrophoresis plastic recycling"
}
};