Team:Amazonas Brazil
CRISPeasy:
Building a standard BioBrick vector for bacterial genome editing easy as A, B, C… CRISPR
The building block of Synthetic Biology is to turn biological system easier to engineer. Biological part standardization is a fundamental key for that goal. CRISPR system highlighted a new scientific revolution. sgRNA specificity associated to the Cas9 programmable activity pave the way for precisely manipulation the genome sequences. However, the synthetic biology community have major obstacles: CRISPR/Cas9 protocol is not standardized and requires laborious time of wet lab work due to the multi-plasmid system. In 2017, our proposal is to develop a novel solution for this core problem, we aim to build a framework for a simple bacterial genome editing based on one standard BioBrick vector. Our perspective is to provide a bacterial genome editing machinery based on BioBrick parts assembly easy to engineer as A, B, C… CRISPR.
