Basic Parts
Our team used several basic parts to create our gene constructs. Here is an overview of all the individual parts that our team attempted to work with, each with a description of the part, its function, and what we did with them.
| Registry link |
Part |
Plasmid |
Summary |
Approximate Length |
| BBa_K2404000 |
TSH |
pSB1C3 |
A gene that produces a protein which competitively inhibits TSH and auto-immune antibodies in Graves' disease. This variant does not have His-tags for purification |
780bp |
| BBa_K2404001 |
TSHH |
pSB1C3 |
A gene that produces produces a protein which competitively inhibits TSH and auto-immune antibodies in Graves' disease. This variant does have His-tags for purification |
832bp |
| BBa_K2404002 |
PDF1.2 |
pSB1C3 |
An inducible promoter that responds to the presence of jasmonic acid in the cell |
522bp |
| BBa_K2404003 |
PR2 |
pSB1C3 |
An inducible promoter that responds to the presence of salicylic acid in the cell |
519bp |
| BBa_K2404004 |
GST6 |
pSB1C3 |
An inducible promoter that responds to the presence of salicylic acid in the cell |
519bp |
| BBa_K2404005 |
WRKY30 |
pSB1C3 |
An inducible promoter that responds to the presence of cellulose-derived oligomers |
507bp |
| BBa_K2404006 |
Luc+ |
pSB1C3 |
A protein coding sequence of a gene that produces an enzyme which creates light when in the presence of its substrate,
luciferin, which can be used to quantify gene expression |
1653bp |
| BBa_K2404007 |
PR2-PDF1.2 |
pSB1C3 |
A composite promoter of both the salicylic acid responsive PR2 at the most 5' end, and of the jasmonic acid responsive PDF1.2 at the most 3' end |
~length |
| BBa_K2404008 |
PR2-GST6 |
pSB1C3 |
A composite promoter of both the salicylic acid responsive PR2 at the most 5' end, and of the salicylic acid responsive GST6 at the most 3' end |
~length |
| BBa_K2404009 |
PDF1.2-GST6 |
pSB1C3 |
A composite promoter of both the jasmonic acid responsive PDF1.2 at the most 5' end, and of the salicylic acid responsive GST6 at the most 3' end |
~length |
| BBa_P10401 |
NosT |
pSB1C3 |
A terminator from the nopaline synthase gene which is native to the Agrobacterium tumor-inducing plasmid pTiT37 |
285bp |
| BBa_P10000 |
LexA |
pSB1C3 |
An estradiol inducible promoter from the LexA gene which is native to Escherichia coli |
362bp |
| BBa_P10003 |
35S-OTMV |
pSB1C3 |
A constitutive promoter, commonly used in genetic modification of organisms, which is native to the Cauliflower Mosaic Virus |
1446bp |
